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Full-Text Articles in Medicine and Health Sciences
Nectin-2-Mediated Entry Of A Syncytial Strain Of Herpes Simplex Virus Via Ph-Independent Fusion With The Plasma Membrane Of Chinese Hamster Ovary Cells, Mark G. Delboy, Jennifer L. Patterson, Aimee M. Hollander, Anthony V. Nicola
Nectin-2-Mediated Entry Of A Syncytial Strain Of Herpes Simplex Virus Via Ph-Independent Fusion With The Plasma Membrane Of Chinese Hamster Ovary Cells, Mark G. Delboy, Jennifer L. Patterson, Aimee M. Hollander, Anthony V. Nicola
Microbiology and Immunology Publications
Background
Herpes simplex virus (HSV) can utilize multiple pathways to enter host cells. The factors that determine which route is taken are not clear. Chinese hamster ovary (CHO) cells that express glycoprotein D (gD)-binding receptors are model cells that support a pH-dependent, endocytic entry pathway for all HSV strains tested to date. Fusion-from-without (FFWO) is the induction of target cell fusion by addition of intact virions to cell monolayers in the absence of viral protein expression. The receptor requirements for HSV-induced FFWO are not known. We used the syncytial HSV-1 strain ANG path as a tool to evaluate the complex …