Medicine and Health Sciences Commons^™

Determination Of Tramadol In Human Plasma By Hplc With Fluorescence Detection, Daniela Luiza Baconi, Miriana Stan, Zainab Abdul Jalil Ebrahim, Cristian Tuchila, Cristian Balalau

Journal of Mind and Medical Sciences

Tramadol is a centrally acting analgesic, atypical opioid, and although it is generally considered as a medicinal drug with a low potential for dependence, there is growing evidence of tramadol abuse in some countries. The ultraviolet detection is not suitable for analysis of tramadol in plasma, due to the lack of sensitivity and selectivity. However, it was shown that tramadol has a weak fluorescence, and the latest techniques for determination of tramadol in plasma include liquid chromatographic methods with fluorescence detection (FL). The objective of the paper was to develop a HPLC-FL method applicable for quantification of tramadol in human …