Medicine and Health Sciences Commons^™

Metformin Alleviates Hepatic Iron Overload And Ferroptosis Through Ampk-Ferroportin Pathway In Hfd-Induced Nafld, Fangzhi Yue, Ying Shi, Shanyu Wu, Lin Xing, Dan He, Lin Wei, Anqi Qiu, Ryan D. Russell, Dongmei Zhang

Health & Human Performance Faculty Publications and Presentations

---

Highlights

• Metformin alleviates HIO and ferroptosis in HFD-induced NAFLD

• FPN is involved in the molecular mechanism of metformin on HIO in HFD-induced NAFLD

• Metformin upregulates FPN expression by reducing lysosomal ubiquitination degradation

Summary

Metformin prevents progression of non-alcoholic fatty liver disease (NAFLD). However, the potential mechanism is not entirely understood. Ferroptosis, a recently recognized nonapoptotic form of regulated cell death, has been reported to be involved in the pathogenesis of NAFLD. Here, we investigated the effects of metformin on ferroptosis and its potential mechanism in NAFLD. We found that metformin prevented the progression of NAFLD, and alleviated hepatic iron …

---

Chronic Jet Lag Alters Gut Microbiome And Mycobiome And Promotes The Progression Of Mafld In Hfhfd-Fed Mice, Ruoyi Zheng, Xingwei Xiang, Ying Shi, Anqi Qiu, Xin Luo, Junyan Xie, Ryan D. Russell, Dongmei Zhang

Health & Human Performance Faculty Publications and Presentations

---

Metabolic dysfunction-associated fatty liver disease (MAFLD) is the most common chronic liver disease worldwide. Circadian disruptors, such as chronic jet lag (CJ), may be new risk factors for MAFLD development. However, the roles of CJ on MAFLD are insufficiently understood, with mechanisms remaining elusive. Studies suggest a link between gut microbiome dysbiosis and MAFLD, but most of the studies are mainly focused on gut bacteria, ignoring other components of gut microbes, such as gut fungi (mycobiome), and few studies have addressed the rhythm of the gut fungi. This study explored the effects of CJ on MAFLD and its related microbiotic …

---

The Decline Of Physical Activity With Age In School-Aged Children With Cerebral Palsy: A Single-Center Cross-Sectional Observational Study, Jinuk Lee, Min-Hwa Suk, Soojin Yoo, Jeong-Yi Kwon

Health & Human Performance Faculty Publications and Presentations

---

Maintaining physical activity is important for children with cerebral palsy (CP). This study examined whether age predicted habitual physical activity (HPA) or cardiorespiratory fitness (CRF) in school-aged children with CP and clarified the relationship between HPA and CRF. We utilized cross-sectional data from 39 children with CP (18 girls and 21 boys; mean age 7.44 years; mean body weight 24.76 kg; mean body mass index 15.97 kg/m2; hemiplegic or diplegic CP). The participants wore an accelerometer (ActiGraph) for five days to measure HPA, physical activity energy expenditure (kcal/kg/d), sedentary physical activity (%SPA), light physical activity, moderate-to-vigorous physical activity (%MVPA), and …

---

Circulating Microrna Responses To Acute Whole-Body Vibration And Resistance Exercise In Postmenopausal Women, Samuel R. Buchanan, Ryan M. Miller, Michelle Nguyen, Christopher D. Black, J. Mikhail Kellawan, Michael G. Bemben, Debra A. Bemben

Health & Human Performance Faculty Publications and Presentations

---

Evaluating alterations in circulating microRNA (c-miRNA) expression may provide deeper insight into the role of exercise in the attenuation of the negative effects of aging on musculoskeletal health. Currently, there are sparse data on c-miRNA responses to acute exercise in postmenopausal women. The purpose of this study was to characterize the effects of acute bouts of resistance exercise and whole-body vibration on expression of selected c-miRNAs in postmenopausal women aged 65-76 years (n=10). We also examined relationships between c-miRNAs and muscle strength and bone characteristics. This randomized crossover design study compared c-miRNA responses to a bout of resistance exercise (RE) …

---

Chronic Constant Light Exposure Aggravates High Fat Diet-Induced Renal Injury In Rats, Lin Xing, Shanyu Wu, Ying Shi, Fangzhi Yue, Lin Wei, Ryan D. Russell, Dongmei Zhang

Health & Human Performance Faculty Publications and Presentations

---

Obesity-related kidney disease is now recognized as a global health issue, with a substantial number of patients developing progressive renal failure and end-stage renal disease. Interestingly, recent studies indicate light pollution is a novel environmental risk factor for chronic kidney disease. However, the impact of light pollution on obesity-related kidney disease remains largely unknown, with its underlying mechanism insufficiently explained. Renal hypoxia induced factor 1α (HIF1α) is critical in the development of glomerulosclerosis and renal fibrosis. The present study explored effects of constant light exposure on high fat diet (HFD) -induced renal injury and its association with HIF1α signal pathway. …

---

Bone-Regulating Micrornas And Resistance Exercise: A Mini-Review, Debra A. Bemben, Zhaojing Chen, Samuel R. Buchanan

Health & Human Performance Faculty Publications and Presentations

---

MicroRNAs (miRNA) are a class of short noncoding RNA that play important roles in controlling gene expression. Many miRNAs have been identified as being important regulators of bone cell function, thus affecting the bone remodeling processes. In addition to being expressed in specific tissues and exerting intracellular effects, miRNAs can enter the blood where they can be taken up by other tissues. These circulating miRNAs (c-miRNA) also have clinical significance as biomarkers of musculoskeletal diseases as they are tissue-specific, are stable and easily detectable, and require minimally invasive procedures. This mini-review discusses miRNAs with regulatory roles in bone metabolism and …

---

The Evolution Of Blood Flow Restricted Exercise, Eduardo D. S. Freitas, Murat Karabulut, Michael G. Bemben

Health & Human Performance Faculty Publications and Presentations

---

The use of blood flow restricted (BFR) exercise has become an accepted alternative approach to improve skeletal muscle mass and function and improve cardiovascular function in individuals that are not able to or do not wish to use traditional exercise protocols that rely on heavy loads and high training volumes. BFR exercise involves the reduction of blood flow to working skeletal muscle by applying a flexible cuff to the most proximal portions of a person's arms or legs that results in decreased arterial flow to the exercising muscle and occluded venous return back to the central circulation. Safety concerns, especially …

---

Impaired Postprandial Skeletal Muscle Vascular Responses To A Mixed Meal Challenge In Normoglycaemic People With A Parent With Type 2 Diabetes, Ryan D. Russell, Katherine M. Roberts-Thomson, Donghua Hu, Timothy Greenaway, Andrew C. Betik, Lewan Parker, James E. Sharman, Stephen M. Richards, Stephen Rattigan, Dino Premilovac, Glenn D. Wadley, Michelle A. Keske

Health & Human Performance Faculty Publications and Presentations

---

Aims/hypothesis: Microvascular blood flow (MBF) increases in skeletal muscle postprandially to aid in glucose delivery and uptake in muscle. This vascular action is impaired in individuals who are obese or have type 2 diabetes. Whether MBF is impaired in normoglycaemic people at risk of type 2 diabetes is unknown. We aimed to determine whether apparently healthy people at risk of type 2 diabetes display impaired skeletal muscle microvascular responses to a mixed-nutrient meal.

Methods: In this cross-sectional study, participants with no family history of type 2 diabetes (FH-) for two generations (n = 18), participants with a positive family history …

---

The Association Between Motor Capacity And Motor Performance In School-Aged Children With Cerebral Palsy: An Observational Study, Min-Hwa Suk, In-Kyeong Park, Soojin Yoo, Jeong-Yi Kwon

Health & Human Performance Faculty Publications and Presentations

---

Background

This study aimed to investigate the association between motor capacity and motor performance in children with cerebral palsy (CP) aged 6–12 years with Gross Motor Function Classification System (GMFCS) levels I to III.

Methods

Forty-six children with CP (24 boys and 22 girls) classified as GMFCS levels Ⅰ, Ⅱ, or Ⅲ were included. Motor capacity was measured by the Gross motor function measure (GMFM), Pediatric balance scale (PBS), Timed up and go (TUG), and 6-min walk test (6MWT). Motor performance was measured by triaxial accelerometers. Estimations of physical activity energy expenditure (PAEE) (kcal/kg/day), percentage of time spent on …

---

Muscle Performance Changes With Age In Active Women, Ryan M. Miller, Eduardo D. S. Freitas, Aaron D. Heishman, Keldon M. Peak, Samuel R. Buchanan, J. Mikhail Kellawan, Hugo M. Pereira, Debra A. Bemben, Michael G. Bemben

Health & Human Performance Faculty Publications and Presentations

---

The purpose of this study was to examine age-related differences in muscle performance in women divided into young (YW, 20–39 years, n = 29) middle-aged (MAW, 40–59 years, n = 33), and older (OW, ≥60 years, n = 40) age groups. Methods: Hand grip strength, vertical jump performance, and knee extensor (KE) strength (0 deg/s, 60 deg/s, and 240 deg/s), speed of movement (SoM; at 1 Nm, 20%, 40%, and 60% isometric strength), and endurance (30-repetition test at 60 degs/s and 240 deg/s) were assessed. Computed tomography-acquired muscle cross-sectional area (mCSA) was measured and included to determine specific strength (KE …

---

Hemodynamic Response And Pulse Wave Analysis After Upper- And Lower-Body Resistance Exercise With And Without Blood Flow Restriction, Yu Lun Tai, Erica M. Marshall, Jason C. Parks, J. Derek Kingsley

Health & Human Performance Faculty Publications and Presentations

---

Resistance exercise (RE) has been shown to elevate hemodynamics and pulse wave reflection. However, the effects of acute RE with blood flow restriction (BFR) on hemodynamics and pulse wave reflection are unclear. The purpose of this study was to evaluate the differences between upper- and lower-body RE with and without BFR on hemodynamics and pulse wave reflection. Twenty-three young resistance-trained individuals volunteered for the study. Hemodynamics and pulse wave reflection were assessed at rest, 10, 25, 40, and 55 minutes after either upper- or lower-body with or without BFR. The upper-body RE (URE) consisted of the latissimus dorsi pulldown and …

---

Constant Light Exposure Alters Gut Microbiota And Promotes The Progression Of Steatohepatitis In High Fat Diet Rats, Lin Wei, Fangzhi Yue, Lin Xing, Shanyu Wu, Ying Shi, Jinchen Li, Xingwei Xiang, Sin Man Lam, Guanghou Shui, Ryan D. Russell, Dongmei Zhang

Health & Human Performance Faculty Publications and Presentations

---

Background: Non-alcoholic fatty liver disease (NAFLD) poses a significant health concern worldwide. With the progression of urbanization, light pollution may be a previously unrecognized risk factor for NAFLD/NASH development. However, the role of light pollution on NAFLD is insufficiently understood, and the underlying mechanism remains unclear. Interestingly, recent studies indicate the gut microbiota affects NAFLD/NASH development. Therefore, the present study explored effects of constant light exposure on NAFLD and its related microbiotic mechanisms.

Material and method: Twenty-eight SD male rats were divided into four groups (n=7 each): rats fed a normal chow diet, and exposed to standard light-dark cycle (ND-LD); …

---

Muscle-Bone Interactions In Chinese Men And Women Aged 18–35 Years, Meihua Su, Zhaojing Chen, Breanne Baker, Samuel R. Buchanan, Debra A. Bemben, Michael G. Bemben

Health & Human Performance Faculty Publications and Presentations

---

To characterize bone mineral density (BMD), bone strength, muscle and fat mass, and muscle strength and power in Chinese women (n = 25) and men (n = 28) classified as in the bone accrual phase (18–25 years) or in the peak bone mass phase (26–35 years). Calcium intakes, physical activity levels, and serum vitamin D were measured. Dual-energy X-ray absorptiometry (DXA) assessed body composition, lumbar spine, and hip areal BMD (aBMD) variables and peripheral quantitative computed tomography (pQCT) assessed cortical and trabecular volumetric BMD (vBMD) and bone strength. Muscle strength and power were assessed by grip strength, leg …

---

Weight Loss And Weight Gain Among Participants In A Community-Based Weight Loss Challenge, Merrill D. Funk, Minjae Lee, Michelle L. Vidoni, Belinda M. Reininger

Health & Human Performance Faculty Publications and Presentations

---

Background

To describe the characteristics of participants who registered for multiple annual offerings of a community-based weight loss program called The Challenge, and to determine participant characteristics associated with weight change over multiple offerings of The Challenge occurring during the years 2010–2016.

Methods

Multivariable linear mixed effects analyses were conducted to describe percent weight change within and between offerings of The Challenge by participant characteristics.

Results

There were 669 and 575 participants included in the within and between analyses, respectively, for offerings of The Challenge. Among the 434 participants who lost weight in their first attempt at The Challenge and …

---

Oral Glucose Challenge Impairs Skeletal Muscle Microvascular Blood Flow In Healthy People, Ryan D. Russell, Donghua Hu, Timothy Greenaway, James E. Sharman, Stephen Rattigan, Stephen M. Richards, Michelle A. Keske

Health & Human Performance Faculty Publications and Presentations

---

Skeletal muscle microvascular (capillary) blood flow increases in the postprandial state or during insulin infusion due to dilation of precapillary arterioles to augment glucose disposal. This effect occurs independently of changes in large artery function. However, acute hyperglycemia impairs vascular function, causes insulin to vasoconstrict precapillary arterioles, and causes muscle insulin resistance in vivo. We hypothesized that acute hyperglycemia impairs postprandial muscle microvascular perfusion, without disrupting normal large artery hemodynamics, in healthy humans. Fifteen healthy people (5 F/10 M) underwent an oral glucose challenge (OGC, 50 g glucose) and a mixed-meal challenge (MMC) on two separate occasions (randomized, crossover design). …

---

Validity Of Wrist-Worn Consumer Products To Measure Heart Rate And Energy Expenditure, R. S. Thiebaud, Merrill D. Funk, Jacelyn C. Patton, Brook L. Massey, Terri E. Shay, Martin G. Schmidt, Nicolas Giovannitti

Health & Human Performance Faculty Publications and Presentations

---

Introduction: The ability to monitor physical activity throughout the day and during various activities continues to improve with the development of wrist-worn monitors. However, the accuracy of wrist-worn monitors to measure both heart rate and energy expenditure during physical activity is still unclear. The purpose of this study was to determine the accuracy of several popular wrist-worn monitors at measuring heart rate and energy expenditure.

Methods: Participants wore the TomTom Cardio, Microsoft Band and Fitbit Surge on randomly assigned locations on each wrist. The maximum number of monitors per wrist was two. The criteria used for heart rate and energy …

---

Assessing Overweight/Obesity, Dietary Habits, And Physical Activity In Hispanic College Students, Ulku S. Karabulut, Zasha Romero, Phillip Conatser, Murat Karabulut

Health & Human Performance Faculty Publications and Presentations

---

Objectives

This study examined the overweight, obesity, dietary habits, and physical activity among Hispanic college students.

Methods

Eighty seven (n=87, age= 24.03 ± 5.69) Hispanic college students participated in the study. Descriptive and anthropometric measurements including resting heart rate (RHR), resting blood pressure (RBP), height, weight, body mass index (BMI), circumference measurements [waist at narrowest point (Xiphoid), and hip at widest point (Hip), body composition (BC) were collected. Subjects completed the Dietary Screener Questionnaire (DSQ). PA was estimated via Godin’s (2011) Leisure-Time Exercise Questionnaire.

Results

The mean BMI was 27.29±6.20 kg/m², in the overweight range. The mean WC …

---

Mobility Limitations And Fear Of Falling In Non-English Speaking Older Mexican-Americans, Eric G. James, Phillip Conatser, Murat Karabulut, Suzanne G. Leveille, Jeffrey M. Hausdorff, Sarah Cote, Katherine L. Tucker, Bruce Barton, Jonathan F. Bean, Soham Al Snih, Kyriakos S. Markides

Health & Human Performance Faculty Publications and Presentations

---

Objective—To determine whether older Mexican-Americans who cannot speak and/or understand spoken English have higher rates of mobility limitations or fear of falling than their English-speaking counterparts.

Design—We conducted a cross-sectional analysis of 1169 community-dwelling MexicanAmericans aged 72–96 years from the 2000–2001 wave of the Hispanic Established Population for the Epidemiological Study of the Elderly. Mobility limitations were defined as having a Short Physical Performance Battery score ≤9, and fear of falling by participant report of being somewhat, fairly, or very afraid of falling. We determined the rates and odds ratios, for having mobility limitations and fear of falling …

---

Mobility Limitations And Fear Of Falling In Non-English Speaking Older Mexican-Americans, Eric G. James, Phillip Conatser, Murat Karabulut, Suzanne G. Leveille, Jeffrey M. Hausdorff, Sarah Cote, Katherine L. Tucker, Bruce Barton, Jonathan F. Bean, Soham Al Snih, Kyriakos S. Markides

Health & Human Performance Faculty Publications and Presentations

---

Objective

To determine whether older Mexican-Americans who cannot speak and/or understand spoken English have higher rates of mobility limitations or fear of falling than their English-speaking counterparts.

Design

We conducted a cross-sectional analysis of 1169 community-dwelling Mexican-Americans aged 72–96 years from the 2000–2001 wave of the Hispanic Established Population for the Epidemiological Study of the Elderly. Mobility limitations were defined as having a Short Physical Performance Battery score ≤9, and fear of falling by participant report of being somewhat, fairly, or very afraid of falling. We determined the rates and odds ratios, for having mobility limitations and fear of falling …

---