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Full-Text Articles in Medicine and Health Sciences
The Characterization Of Hematopoiesis In Murine Fetal Bone Marrow, Trent Daniel Hall
The Characterization Of Hematopoiesis In Murine Fetal Bone Marrow, Trent Daniel Hall
Theses and Dissertations (ETD)
Hematopoietic stem cells (HSCs) and their downstream progenitors are a heterogeneous population of cells that are indispensable for lifelong hematopoiesis and are often utilized in the clinic for the treatment of hematologic maladies via hematopoietic stem cell transplantation (HSCT). Over several decades, it has been discovered that HSCs arise in the dorsal aorta of the developing embryo, migrate to the fetal liver (FL), and undergo a large expansion before reaching their final resting place in the bone marrow (BM). Many resources have been invested in understanding the roles of the different niches HSCs encounter along their journey. A greater understanding …
Arid1a Haploinsufficiency Initiates Neural Crest Transformation In A Mouse Model Of Mycn-Driven Neuroblastoma, Kirby A. Wallace
Arid1a Haploinsufficiency Initiates Neural Crest Transformation In A Mouse Model Of Mycn-Driven Neuroblastoma, Kirby A. Wallace
Theses and Dissertations (ETD)
Mouse models of cancer are critical for developing therapeutic treatments for pediatric patients. Recent sequencing studies of neuroblastoma (NBL) patient tumors have uncovered precise deletions in the chromatin remodeler and tumor suppressor gene (TSG) ARID1A. Additional causal studies supported ARID1A’s candidacy as a putative 1p36 TSG in MYCN-driven NBL. This study aimed to causally test Arid1a loss during Mycn-driven NBL initiation through the development of a mouse model of high risk NBL.In this study, we modified a Mycn-driven mouse model of NBL to incorporate Cre mediated deletion of floxed Arid1a. Briefly, in freshly isolated embryonic day 9.5 primary trunk neural …