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Development Of A Mel Cell-Derived Allograft Mouse Model For Cancer Research, Min Young Kim, Sungwoo Choi, Seol Eui Lee, Ji Sook Kim, Seung Han Son, Young Soo Lim, Bang-Jin Kim, Buom-Yong Ryu, Vladimir N. Uversky, Young Jin Lee, Chul Geun Kim

Molecular Medicine Faculty Publications

Murine erythroleukemia (MEL) cells are often employed as a model to dissect mechanisms of erythropoiesis and erythroleukemia in vitro. Here, an allograft model using MEL cells resulting in splenomegaly was established to develop a diagnostic model for isolation/quantification of metastatic cells, anti-cancer drug screening, and evaluation of the tumorigenic or metastatic potentials of molecules in vivo. In this animal model, circulating MEL cells from the blood stream were successfully isolated and quantified with an additional in vitro cultivation step. In terms of the molecular-pathological analysis, we were able to successfully evaluate the functional discrimination between methyl-CpG-binding domain 2 (Mbd2) and …