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Immunogenicity And Protective Activity Of A Chimeric Protein Based On The Domain Iii Of The Tick-Borne Encephalitis Virus E Protein And The Ompf Porin Of Yersinia Pseudotuberculosis Incorporated Into The Ti-Complex, Nina M. Sanina, Natalia Chopenko, Andrey Mazeika, Ludmila Davydova, Galina Leonova, Anna Stenkova, Vladimir N. Uversky, Eduard Kostetsky

Molecular Medicine Faculty Publications

Tick-borne encephalitis (TBE) is a widespread, dangerous infection. Unfortunately, all attempts to create safe anti-TBE subunit vaccines are still unsuccessful due to their low immunogenicity. The goal of the present work was to investigate the immunogenicity of a recombinant chimeric protein created by the fusion of the EIII protein, comprising domain III and a stem region of the tick-borne encephalitis virus (TBEV) E protein, and the OmpF porin of Yersinia pseudotuberculosis (OmpF-EIII). Adjuvanted antigen delivery systems, the tubular immunostimulating complexes (TI-complexes) based on the monogalactosyldiacylglycerol from different marine macrophytes, were used to enhance the immunogenicity of OmpF-EIII. Also, the chimeric …