Medicine and Health Sciences Commons^™

Osmolyte-Like Stabilizing Effects Of Low Gdnhcl Concentrations On D-Glucose/D-Galactose-Binding Protein, Alexander V. Fonin, Alexandra D. Golikova, Irina A. Zvereva, Sabato D’Auria, Maria Staiano, Vladimir N. Uversky, Irina M. Kuznetsova, Konstantin K. Turoverov

Molecular Medicine Faculty Publications

The ability of d-glucose/d-galactose-binding protein (GGBP) to reversibly interact with its ligands, glucose and galactose, makes this protein an attractive candidate for sensing elements of glucose biosensors. This potential is largely responsible for attracting researchers to study the conformational properties of this protein. Previously, we showed that an increase in the fluorescence intensity of the fluorescent dye 6-bromoacetyl-2-dimetylaminonaphtalene (BADAN) is linked to the holo-form of the GGBP/H152C mutant in solutions containing sub-denaturing concentrations of guanidine hydrochloride (GdnHCl). It was hypothesized that low GdnHCl concentrations might lead to compaction of the protein, thereby facilitating ligand binding. In this work, we utilize …