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Full-Text Articles in Medicine and Health Sciences
Identifying Novel Cell Cycle Proteins In Apicomplexa Parasites Through Co-Expression Decision Analysis, Carrie L. Butler, Olivier Lucas, Stefan Wuchty, Bin Xue, Vladimir N. Uversky, Michael White
Identifying Novel Cell Cycle Proteins In Apicomplexa Parasites Through Co-Expression Decision Analysis, Carrie L. Butler, Olivier Lucas, Stefan Wuchty, Bin Xue, Vladimir N. Uversky, Michael White
Molecular Medicine Faculty Publications
No abstract provided.
Genetic Manipulation Of The Toxoplasma Gondii Genome By Fosmid Recombineering, Sumiti Vinayak, Carrie F. Brooks, Anatoli Naumov, Elena Suvorova, Michael W. White, Boris Striepen
Genetic Manipulation Of The Toxoplasma Gondii Genome By Fosmid Recombineering, Sumiti Vinayak, Carrie F. Brooks, Anatoli Naumov, Elena Suvorova, Michael W. White, Boris Striepen
Molecular Medicine Faculty Publications
Apicomplexa are obligate intracellular parasites that cause important diseases in humans and animals. Manipulating the pathogen genome is the most direct way to understand the functions of specific genes in parasite development and pathogenesis. In Toxoplasma gondii, nonhomologous recombination is typically highly favored over homologous recombination, a process required for precise gene targeting. Several approaches, including the use of targeting vectors that feature large flanks to drive site-specific recombination, have been developed to overcome this problem. We have generated a new large-insert repository of T. gondii genomic DNA that is arrayed and sequenced and covers 95% of all of the …
Pe-Db: A Database Of Structural Ensembles Of Intrinsically Disordered And Unfolded Proteins, Mihaly Varadi, Simone Kosol, Pierre Lebrun, Erica Valentini, Martin Blackledge, A. Keith Dunker, Isabella C. Felli, Julie D. Forman-Kay, Richard Kriwacki, Roberta Pierattelli, Joel Sussman, Dmitri I. Svergun, Vladimir N. Uversky, Michele Vendruscolo, David Wishart, Peter E. Wright, Peter Tompa
Pe-Db: A Database Of Structural Ensembles Of Intrinsically Disordered And Unfolded Proteins, Mihaly Varadi, Simone Kosol, Pierre Lebrun, Erica Valentini, Martin Blackledge, A. Keith Dunker, Isabella C. Felli, Julie D. Forman-Kay, Richard Kriwacki, Roberta Pierattelli, Joel Sussman, Dmitri I. Svergun, Vladimir N. Uversky, Michele Vendruscolo, David Wishart, Peter E. Wright, Peter Tompa
Molecular Medicine Faculty Publications
The goal of pE-DB (http://pedb.vib.be) is to serve as an openly accessible database for the deposition of structural ensembles of intrinsically disordered proteins (IDPs) and of denatured proteins based on nuclear magnetic resonance spectroscopy, small-angle X-ray scattering and other data measured in solution. Owing to the inherent flexibility of IDPs, solution techniques are particularly appropriate for characterizing their biophysical properties, and structural ensembles in agreement with these data provide a convenient tool for describing the underlying conformational sampling. Database entries consist of (i) primary experimental data with descriptions of the acquisition methods and algorithms used for the ensemble …
Intrinsic Disorder In The Bk Channel And Its Interactome, Zhenling Peng, Yoshihisa Sakai, Lukasz Kurgan, Bernd Sokolowski, Vladimir N. Uversky
Intrinsic Disorder In The Bk Channel And Its Interactome, Zhenling Peng, Yoshihisa Sakai, Lukasz Kurgan, Bernd Sokolowski, Vladimir N. Uversky
Molecular Medicine Faculty Publications
The large-conductance Ca2+-activated K+ (BK) channel is broadly expressed in various mammalian cells and tissues such as neurons, skeletal and smooth muscles, exocrine cells, and sensory cells of the inner ear. Previous studies suggest that BK channels are promiscuous binders involved in a multitude of protein-protein interactions. To gain a better understanding of the potential mechanisms underlying BK interactions, we analyzed the abundance, distribution, and potential mechanisms of intrinsic disorder in 27 BK channel variants from mouse cochlea, 104 previously reported BK-associated proteins (BKAPS) from cytoplasmic and membrane/cytoskeletal regions, plus BK β- and γ-subunits. Disorder was evaluated using the MFDp …