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Articles 1 - 9 of 9
Full-Text Articles in Medicine and Health Sciences
Archaic Chaos: Intrinsically Disordered Proteins In Archaea, Bin Xue, Robert W. Williams, Christopher J. Oldfield, A. Keith Dunker, Vladimir N. Uversky
Archaic Chaos: Intrinsically Disordered Proteins In Archaea, Bin Xue, Robert W. Williams, Christopher J. Oldfield, A. Keith Dunker, Vladimir N. Uversky
Molecular Medicine Faculty Publications
Background: Many proteins or their regions known as intrinsically disordered proteins (IDPs) and intrinsically disordered regions (IDRs) lack unique 3D structure in their native states under physiological conditions yet fulfill key biological functions. Earlier bioinformatics studies showed that IDPs and IDRs are highly abundant in different proteomes and carry out mostly regulatory functions related to molecular recognition and signal transduction. Archaea belong to an intriguing domain of life whose members, being microbes, are characterized by a unique mosaic-like combination of bacterial and eukaryotic properties and include inhabitants of some of the most extreme environments on the planet. With the expansion …
Calbindin-D28k Acts As A Calcium-Dependent Chaperone Suppressing Α-Synuclein Fibrillation In Vitro, Wenbo Zhou, Chunmei Long, Anthony L. Fink, Vladimir N. Uversky
Calbindin-D28k Acts As A Calcium-Dependent Chaperone Suppressing Α-Synuclein Fibrillation In Vitro, Wenbo Zhou, Chunmei Long, Anthony L. Fink, Vladimir N. Uversky
Molecular Medicine Faculty Publications
α-Synuclein, a natively unfolded protein aggregation which is implicated in the pathogenesis of Parkinson’s disease and several other neurodegenerative diseases, is known to interact with a great number of unrelated proteins. Some of these proteins, such as ß-synuclein and DJ-1, were shown to inhibit α-synuclein aggregation in vitro and in vivo therefore acting as chaperones. Since calbindin-D28K is co-localized with Ca2+ neuronal membrane pumps, and since α-synuclein is also found in the membrane proximity, these two proteins can potentially interact in vivo. Here we show that calbindin-D28K interacts with α-synuclein and inhibits its fibrillation in a calcium-dependent manner, therefore potentially …
N-Terminal Domains Of Della Proteins Are Intrinsically Unstructured In The Absence Of Interaction With Gid1/Gibberellic Acid Receptors, Xiaolin Sun, William T. Jones, Dawn Harvey, Patrick J. B. Edwards, Steven M. Pascale, Christopher Kirk, Thérèse Considine, David J. Sheerin, Jasna Rakonjac, Christopher J. Oldfield, Bin Xue, A. Keith Dunker, Vladimir N. Uversky
N-Terminal Domains Of Della Proteins Are Intrinsically Unstructured In The Absence Of Interaction With Gid1/Gibberellic Acid Receptors, Xiaolin Sun, William T. Jones, Dawn Harvey, Patrick J. B. Edwards, Steven M. Pascale, Christopher Kirk, Thérèse Considine, David J. Sheerin, Jasna Rakonjac, Christopher J. Oldfield, Bin Xue, A. Keith Dunker, Vladimir N. Uversky
Molecular Medicine Faculty Publications
The plant growth-repressing DELLA proteins (DELLAs) are known to represent a convergence point in integration of multiple developmental and environmental signals in planta, one of which is hormone gibberellic acid (GA). Binding of the liganded GA receptor (GID1/GA) to the N-terminal domain of DELLAs is required for GA-induced degradation of DELLAs via the ubiquitin-proteasome pathway, thus derepressing plant growth. However, the conformational changes of DELLAs upon binding to GID1/GA, which are the key to understanding the precise mechanism of GID1/GA-mediated degradation of DELLAs, remain unclear. Using biophysical, biochemical, and bioinformatics approaches, we demonstrated for the first time that the …
Share On Role Of An Expanded Inositol Transporter Repertoire In Cryptococcus Neoformans Sexual Reproduction And Virulence, Chaoyang Xue, Tongbao Liu, Lydia Chen, Wenjun Li, Iris Liu, James W. Kronstad, Andreas Seyfang, Joseph Heitman
Share On Role Of An Expanded Inositol Transporter Repertoire In Cryptococcus Neoformans Sexual Reproduction And Virulence, Chaoyang Xue, Tongbao Liu, Lydia Chen, Wenjun Li, Iris Liu, James W. Kronstad, Andreas Seyfang, Joseph Heitman
Molecular Medicine Faculty Publications
Cryptococcus neoformans and Cryptococcus gattii are globally distributed human fungal pathogens and the leading causes of fungal meningitis. Recent studies reveal that myo-inositol is an important factor for fungal sexual reproduction. That C. neoformans can utilize myo-inositol as a sole carbon source and the existence of abundant inositol in the human central nervous system suggest that inositol is important for Cryptococcus development and virulence. In accord with this central importance of inositol, an expanded myo-inositol transporter (ITR) gene family has been identified in Cryptococcus. This gene family contains two phylogenetically distinct groups, with a …
Fluorescence Quantum Yield Of Thioflavin T In Rigid Isotropic Solution And Incorporated Into The Amyloid Fibrils, Anna I. Sulatskaya, Alexander A. Maskevich, Irina M. Kuznetsova, Vladimir N. Uversky, Konstantin K. Turoverov
Fluorescence Quantum Yield Of Thioflavin T In Rigid Isotropic Solution And Incorporated Into The Amyloid Fibrils, Anna I. Sulatskaya, Alexander A. Maskevich, Irina M. Kuznetsova, Vladimir N. Uversky, Konstantin K. Turoverov
Molecular Medicine Faculty Publications
In this work, the fluorescence of thioflavin T (ThT) was studied in a wide range of viscosity and temperature. It was shown that ThT fluorescence quantum yield varies from 0.0001 in water at room temperature to 0.28 in rigid isotropic solution (T/η→0). The deviation of the fluorescence quantum yield from unity in rigid isotropic solution suggests that fluorescence quantum yield depends not only on the ultra-fast oscillation of ThT fragments relative to each other in an excited state as was suggested earlier, but also depends on the molecular configuration in the ground state. This means that the fluorescence …
Retro-Morfs: Identifying Protein Binding Sites By Normal And Reverse Alignment And Intrinsic Disorder Prediction, Bin Xue, A. Keith Dunker, Vladimir N. Uversky
Retro-Morfs: Identifying Protein Binding Sites By Normal And Reverse Alignment And Intrinsic Disorder Prediction, Bin Xue, A. Keith Dunker, Vladimir N. Uversky
Molecular Medicine Faculty Publications
Many cell functions in all living organisms rely on protein-based molecular recognition involving disorder-to-order transitions upon binding by molecular recognition features (MoRFs). A well accepted computational tool for identifying likely protein-protein interactions is sequence alignment. In this paper, we propose the combination of sequence alignment and disorder prediction as a tool to improve the confidence of identifying MoRF-based protein-protein interactions. The method of reverse sequence alignment is also rationalized here as a novel approach for finding additional interaction regions, leading to the concept of a retro-MoRF, which has the reversed sequence of an identified MoRF. The set of retro-MoRF binding …
A Bimodal Distribution Of Two Distinct Categories Of Intrinsically Disordered Structures With Separate Functions In Fg Nucleoporins, Justin Yamada, Joshua L. Philips, Samir Patel, Gabriel Goldfien, Alison Calestagne-Morelli, Hans Huang, Ryan Reza, Justin Acheson, Viswanathan V. Krishnan, Shawn Newsam, Ajay Gopinathan, Edmond Y. Lau, Michael E. Colvin, Vladimir N. Uversky, Michael F. Rexach
A Bimodal Distribution Of Two Distinct Categories Of Intrinsically Disordered Structures With Separate Functions In Fg Nucleoporins, Justin Yamada, Joshua L. Philips, Samir Patel, Gabriel Goldfien, Alison Calestagne-Morelli, Hans Huang, Ryan Reza, Justin Acheson, Viswanathan V. Krishnan, Shawn Newsam, Ajay Gopinathan, Edmond Y. Lau, Michael E. Colvin, Vladimir N. Uversky, Michael F. Rexach
Molecular Medicine Faculty Publications
Nuclear pore complexes (NPCs) gate the only conduits for nucleocytoplasmic transport in eukaryotes. Their gate is formed by nucleoporins containing large intrinsically disordered domains with multiple phenylalanine-glycine repeats (FG domains). In combination, these are hypothesized to form a structurally and chemically homogeneous network of random coils at the NPC center, which sorts macromolecules by size and hydrophobicity. Instead, we found that FG domains are structurally and chemically heterogeneous. They adopt distinct categories of intrinsically disordered structures in non-random distributions. Some adopt globular, collapsed coil configurations and are characterized by a low charge content. Others are highly charged and adopt more …
3, 4-Dihydroxyphenylacetic Acid (Dopac) Impairs Α-Synuclein Interaction With Lipids, Wenbo Zhou, Chunmei Long, Anthony L. Fink, Vladimir N. Uversky
3, 4-Dihydroxyphenylacetic Acid (Dopac) Impairs Α-Synuclein Interaction With Lipids, Wenbo Zhou, Chunmei Long, Anthony L. Fink, Vladimir N. Uversky
Molecular Medicine Faculty Publications
α-Synuclein (α-Syn) is a small intrinsically disordered presynaptic protein known to form insoluble filamentous inclusions in Parkinson’s disease (PD) and other neurodegenerative disorders. Various catecholamines can inhibit the α-Syn fibrillation in vitro. Recently, non-covalent binding of DOPAC (3,4-dihydroxyphenylacetic acid), a normal product of the dopamine metabolism, was shown to inhibit the fibrillation of α-Syn due to the DOPAC-induced stabilization of the normally transient oligomers thus preventing them from subsequent fibril formation (Zhou, et al. J. Mol. Biol. 2009, 388 (3), 597-610). We are showing here that the interaction of DOPAC with α-Syn decreases the binding affinity of α- Syn to …
Effects Of Various Flavonoids On The Α-Synuclein Fibrillation Process, Xiaoyun Meng, Larissa A. Munishkina, Anthony L. Fink, Vladimir N. Uversky
Effects Of Various Flavonoids On The Α-Synuclein Fibrillation Process, Xiaoyun Meng, Larissa A. Munishkina, Anthony L. Fink, Vladimir N. Uversky
Molecular Medicine Faculty Publications
α-Synuclein aggregation and fibrillation are closely associated with the formation of Lewy bodies in neurons and are implicated in the causative pathogenesis of Parkinson's disease and other synucleinopathies. Currently, there is no approved therapeutic agent directed toward preventing the protein aggregation, which has been recently shown to have a key role in the cytotoxic nature of amyloidogenic proteins. Flavonoids, known as plant pigments, belong to a broad family of polyphenolic compounds. Over 4,000 flavonoids have been identified from various plants and foodstuffs derived from plants and have been demonstrated as potential neuroprotective agents. In this study 48 flavonoids belonging to …