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Full-Text Articles in Medicine and Health Sciences
Structural And Functional Adaptations To Extreme Temperatures In Psychrophilic, Mesophilic, And Thermophilic Dna Ligases, Daphné Georlette, Benjamin Damien, Vinciane Blaise, Eric Depiereux, Vladimir N. Uversky, Charles Gerday, Georges Feller
Structural And Functional Adaptations To Extreme Temperatures In Psychrophilic, Mesophilic, And Thermophilic Dna Ligases, Daphné Georlette, Benjamin Damien, Vinciane Blaise, Eric Depiereux, Vladimir N. Uversky, Charles Gerday, Georges Feller
Molecular Medicine Faculty Publications
Psychrophiles, host of permanently cold habitats, display metabolic fluxes comparable to those exhibited by mesophilic organisms at moderate temperatures. These organisms have evolved by producing, among other peculiarities, cold-active enzymes that have the properties to cope with the reduction of chemical reaction rates induced by low temperatures. The emerging picture suggests that these enzymes display a high catalytic efficiency at low temperatures through an improved flexibility of the structural components involved in the catalytic cycle, whereas other protein regions, if not implicated in catalysis, may be even more rigid than their mesophilic counterparts. In return, the increased flexibility leads to …
Cofactor Binding Modulates The Conformational Stabilities And Unfolding Patterns Of Nad+-Dependent Dna Ligases From Escherichia Coli And Thermus Scotoductus, Daphné Georlette, Vinciane Blaise, Christophe Dohmen, Fabrice Bouillenne, Benjamin Damien, Eric Depiereux, Charles Gerday, Vladimir N. Uversky, Georges Feller
Cofactor Binding Modulates The Conformational Stabilities And Unfolding Patterns Of Nad+-Dependent Dna Ligases From Escherichia Coli And Thermus Scotoductus, Daphné Georlette, Vinciane Blaise, Christophe Dohmen, Fabrice Bouillenne, Benjamin Damien, Eric Depiereux, Charles Gerday, Vladimir N. Uversky, Georges Feller
Molecular Medicine Faculty Publications
DNA ligases are important enzymes required for cellular processes such as DNA replication, recombination, and repair. NAD+-dependent DNA ligases are essentially restricted to eubacteria, thus constituting an attractive target in the development of novel antibiotics. Although such a project might involve the systematic testing of a vast number of chemical compounds, it can essentially gain from the preliminary deciphering of the conformational stability and structural perturbations associated with the formation of the catalytically active adenylated enzyme. We have, therefore, investigated the adenylation-induced conformational changes in the mesophilic Escherichia coli and thermophilic Thermus scotoductus NAD+-DNA ligases, and …