Medicine and Health Sciences Commons^™

Muscle Fiber Size And Composition Differences Between Ames Dwarf Mice And Wild-Type Mice, Jordan Anderson

Physical Therapy Scholarly Projects

Background: Ames dwarf mice demonstrate delayed aging processes in multiple body systems. We compared muscle composition and area between dwarf and wild-type mice to determine if any significant differences exist.

Methods: We resected soleus, gastrocnemius/plantaris, tibialis anterior, and extensor digitorum longus muscles from both dwarf and wild-type mice, then froze, sliced, set on slides, and stained to isolate fast-twitch and slow-twitch fibers. Microscopy images were taken at 10x magnification and fiber composition and cross-sectional area were measured in soleus muscles.

Results: Wild-type mice had an average composition of 52.3% fast-twitch and 47.7% slow-twitch fibers while dwarf mice had 66.3% slow-twitch …

Sarcopenia And Current Research Using Ames Dwarf Mice: A Research Study, Amanda Sodemann

Physical Therapy Scholarly Projects

This research project focused on the differences observed in muscle fiber composition and surface area between wild type mice and Ames dwarf mice as they aged. A mouse model was utilized because the skeletal muscle framework of mice closely resembles that of human muscle; thus, the observations seen within the mice may also be seen within humans as they age. Comparisons between the wild type and dwarf mice include a smaller cross-sectional muscle area in a dwarf mouse and no drastic change in the percentages of fast and slow twitch muscle fibers as the dwarf mouse ages. Furthermore, differences in …

Fiber Type And Cross-Sectional Area Characteristics In Hindlimb Muscles Of The Long-Lived Ames Dwarf Mouse, Kristine Dahlheimer

Physical Therapy Scholarly Projects

Background: Ageing is associated with sarcopenia and a change of composition in muscle fibers. Ames dwarf mice have been found to have a decreased susceptibility to the deleterious effects of the ageing process, when compared to a normal mouse counterpart. This study looks at the muscle composition of the Ames dwarf mouse compared to the normal mouse by analyzing two muscles, ELD and Sol.

Methods: Researchers harvested hind limb muscles from Ames dwarf mice and normal mice. The fours muscles were EDL, Sol, Gastrocnemius/Plantaris, and TA. EDL and Sol were sliced and stained with fast and slow twitch and H&E …

Fiber Type And Cross-Sectional Area Characteristics In Hindlimb Muscles Of The Long-Lived Ames Dwarf Mouse, David Farder

Physical Therapy Scholarly Projects

Background: Aging is associated with sarcopenia and a change of composition in muscle fibers. Ames dwarf mice have been found to have a decreased susceptibility to the deleterious effects of the aging process, when compared to a normal mouse counterpart. This study looks at the muscle composition of the Ames dwarf mouse compared to the normal mouse by analyzing two muscles, extensor digitorum longus (EDL) and soleus (Sol).

Methods: Researchers harvested hind limb muscles from Ames dwarf mice and normal mice. The fours muscles were EDL, Sol, Gastrocnemius/Plantaris, and tibialis anterior (TA). EDL and Sol were sliced and stained with …

Muscle Fiber Type Characterization In The Ames Dwarf Mouse: A Model Of Delayed Aging, Angela M. Williams

Physical Therapy Scholarly Projects

Introduction: Normal muscle wasting due to aging, known as sarcopenia, has many implications in society, especially with a growing population of elderly people. In this study, muscle fiber size and count, nuclei count, and ratio of fast- to slow-twitch fibers was studied between Ames dwarf mice (df), an established model of aging, and wild-type mice (WT) to learn more about the mechanisms of aging, specifically related to skeletal muscle.

Methods: Four hind limb muscles were harvested from two Ames dwarf mice and two wildtype mice, sliced cross-sectionally and mounted on slides. After mounting, the slides were stained three different ways …

Sarcopenia And Aging: Skeletal Muscle Fiber Type Composition Of The Long-Lived Ames Dwarf Mouse Compared To Its Wild-Type Counterpart, Cory Leier

Physical Therapy Scholarly Projects

Skeletal muscle fiber type composition has not been studied in the long-lived Ames dwarf mouse when compared to its wild-type counterpart. Hindlimb soleus skeletal muscle from two 3-month old dwarf and two 3-month old wild-type mice was analyzed for muscle fiber size, proportion of type I (slow-twitch) and type II (fast-twitch) muscle fibers, myonuclei content per muscle fiber, and total cross sectional area of the muscle. Results revealed a significant difference in muscle fiber size (t(58)=-9.71, p

Progressive Resistive Weight Training And The Deconditioned Elderly Patient, Blaine Fercho

Physical Therapy Scholarly Projects

Physical conditioning to improve levels of functional independence is a key rehabilitation process for many elderly adults. This Independent Study is a review of published information concerning exercise for the elderly population with special attention focused on progressive resistive weight training. The inclusion and participation in exercise has been shown to provide many benefits for improving physiological systems' functioning, and also reduce the systems' natural age decline. Exercise participation is therefore highly beneficial; whether it is aerobic, weight training, or recreational activity. Progressive resistive weight training provides the gains of a strengthening program and allows for endurance/aerobic training through the …