Medicine and Health Sciences Commons^™

Chemotherapeutic Drug Resistance Associated With Differential Mirna Expression Of Mir-375 And Mir-27 Among Oral Cancer Cell Lines, Kieran Huni

UNLV Theses, Dissertations, Professional Papers, and Capstones

Objective: Although progress has been made towards the identification of molecular profiles that influence oral cancer growth and metastasis, recent advances have suggested that non-coding microRNAs (such as miR-21, miR-27, miR-145, miR-155, miR-365, miR-375 and miR-494) may be involved in multiple aspects of chemotherapeutic responsiveness. Based upon the paucity of information regarding this phenomenon, this project will determine whether these specific microRNAs are correlated with oral cancer responsiveness to chemotherapies, including Paclitaxel, Cisplatin and Fluorouracil (5FU).Methods: Commercially available and well-characterized oral squamous cell carcinoma cell lines (SCC4, SCC9, SCC15, SCC25 and CAL27) were used for this study. Experimental 96-well assays …

Oral Prevalence Of Akkermancia Muciniphila Among Pediatric And Adult Orthodontic And Non-Orthodontic Patients, Ching Shen

UNLV Theses, Dissertations, Professional Papers, and Capstones

Akkermansia muciniphila (AM) is one of many highly abundant intestinal microbes that influences homeostasis and metabolic disorders and may also play a role in oral disorders. However, there is little evidence regarding the oral prevalence of this organism. Based upon this lack of evidence, the primary goal of this project is to survey an existing saliva repository to determine the overall prevalence of this organism and any associations with demographic or patient characteristics (age, sex, body mass index, race/ethnicity, orthodontic therapy). Using an approved protocol, a total n = 141 pediatric samples from an existing saliva repository were screened using …

Leveraging Simulation To Understand Nursing Student Learning In Technologically Complex Environment, Deborah Rojas

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction The adoption of simulation technologies in schools of nursing has increased significantly over the last 5 years. Results regarding the use of simulation technology have been positive. The use of simulation technologies has been developed to include complex nursing situations that resemble real clinical settings. Research has demonstrated that memory recall is better in an environment that is the same or similar to the environment in which the learning took place (Krokos et al., 2018). For this reason, educators have used simulation technologies to create realistic environments where students can learn. However, learning outcomes in simulations are affected by …

A Pilot Program To Inform High School Students About Allied Healthcare Professions, Nhu Mac

UNLV Theses, Dissertations, Professional Papers, and Capstones

The literature revealed the lack of diversity in the workforce, limited recruiting and retention methods, and lack of exposure to these professions as the contributions to the shortage and demand for the profession of occupational therapy (OT), physical therapy (PT), and speech- language pathology (SLP). As a result, the fourteen-week program to inform high school students about OT, PT, and SLP roles in the community and public health was implemented at Desert Pines High School in Clark County School District (CCSD) to see if it would influence their perceived attitudes, knowledge, beliefs, and the likelihood of pursuing these careers. Using …

Differential Effects Of Extracellular Matrix Glycoproteins Fibronectin And Laminin-5 On Dental Pulp Stem Cell Phenotypes And Responsiveness, Hyungbin Lee

UNLV Theses, Dissertations, Professional Papers, and Capstones

Dental pulp stem cells (DPSCs) are mesenchymal stem cells (MSCs) with the potential to differentiate in a limited number of other tissue types. Some evidence has suggested the modulation of DPSC growth may be mediated, in part, by exogenous extracellular matrix (ECM) glycoproteins, including fibronectin (FN) and laminin-5 (LN5). Although preliminary research suggests that some ECM glycoproteins may work as functional biomaterials to modulate DPSC growth responses, the primary goal of this project is to determine the specific effects of FN and LN5 on DPSC growth and viability.Using an existing DPSC repository, n = 16 DPSC isolates were cultured and …

Dental Pulp Stem Cell Differentiation Potential Of Bmp-2 And Bmp-4, David Li

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction: Many studies are now evaluating the potential for dental pulp stem cells (DPSC) to assist with more complex and biotechnology applications, such as facilitating and promoting osseointegration following dental implants. However, the effects of factors that may control osseointegration and bone repair using DPSC including bone morphogenic proteins (BMP-2, BMP-4) are not yet well understood.Objective: Based upon this lack of evidence, the primary goal of this project is to evaluate the potential effects of BMPs (alone or in combination with other growth factors) to induce factors associated with osteogenesis. Methods: DPSC isolates from an existing repository (n = 13) …

Self-Reported Covid-19 Diagnosis And Severity And Its Association With Utilization Of And Delays In Needed Medical Care, Mohammad Hesam Alavi

UNLV Theses, Dissertations, Professional Papers, and Capstones

Covid-19, caused by the virus SARS-CoV-2, was declared a pandemic by the World Health Organization (WHO) on March 11, 2020. The pandemic-related disruptions and the pursuant lockdowns have adversely impacted every aspect of people’s lives including access to medical care, cancer care, preventive medicine, mental health, and dental care in an already imperfect healthcare system in the US. This study utilized the 2020 self-reported data from quarters 3 and 4 of the National Health Interview Survey, a cross-sectional interview survey by the Centers for Disease Control and Prevention, to determine the possible association of self-reported COVID diagnosis, test positivity, and …

A Feasibility Study To Identify If The Induction Of The Relationship Based Care Program Enhance The Caring Skills Of Registered Nurses?, Jennifer Millet

UNLV Theses, Dissertations, Professional Papers, and Capstones

The purpose of this Doctor of Nursing Practice project was to educate the Registered Nurses (RNs) to implement the Relationship Based Care (RBC) model and Jean Watson’s Theory of Human Caring principles into their practice. The model and principles were put into practice by the RNs, followed by examining the RNs perception and practices of caring and confidence skills had improved after the intervention. The mission of this project was to promote quality outcomes and patient satisfaction through caring relationships. Strengthening these relationships will help the caregiver experience the essence of care and be able to better connect to another …

Family-Centered Psychosocial Care For Pediatric Oncology Patients, Jessica W. Casey

UNLV Theses, Dissertations, Professional Papers, and Capstones

Psychosocial conditions are prevalent in pediatric oncology patients (Kahalley et al., 2013). Family and social support are essential aspects of a patient's health care outcomes (Merck & McElfresh, 2019). Anxiety and depression are common in pediatric oncology patients due to trauma during treatment (Pao & Kazak, 2015). These patients also can have preexisting developmental or behavioral conditions before starting treatment. Treatment side effects can also cause cognitive and behavioral changes (Nixon, 2014b). Nurses should be knowledgeable and confident in their ability to care for patients' psychosocial health and development. The purpose of this Doctor of Nursing Practice (DNP) project is …

Is Dietary Teaching Via Telehealth Effective In Lowering Hgba1c In Adult Patients With Prediabetes Or Type 2 Diabetes?, Mia Mccallum-Crawford

UNLV Theses, Dissertations, Professional Papers, and Capstones

Prediabetes and Type 2 diabetes mellitus (T2DM) are two common conditions diagnosed in the primary care setting. Both conditions benefit from a healthy lifestyle, managing blood sugar levels, and getting diabetes self-management education and support (DSMES). Within the past two decades, the healthcare community in the US has witnessed a substantial rise in diabetes among adults. Overall, the self-management of diabetes continues to worsen nationally. The purpose of this project is to improve patients’ self-management of both Prediabetes and T2DM through nutrition and show how nutritional counseling via telehealth provided by a nurse practitioner can improve HgbA1C values over 3 …

Comparative Effects Of High-Intensity Interval Warm-Up Exercise And Bronchodilator On Exercise-Induced Bronchoconstriction In Children With Mild Asthma, Michael Wong

UNLV Theses, Dissertations, Professional Papers, and Capstones

Purpose: The troubling link between obesity and asthma in children, along with greater associated detrimental health effects, warrants investigation to better understand the mechanisms driving this relationship as well as its consequences, especially during exercise. Bronchodilators and interval warm-up exercises are recommended by the American Thoracic Society to prevent exercise-induced bronchoconstriction (EIB). Whether interval warm-up exercise can prevent EIB to the same extent as bronchodilators in asthmatic children is unknown but clinically important. Therefore, the first purpose of this study is to investigate the effects of obesity on pulmonary function, exercise tolerance, and exercise-induced bronchoconstriction in asthmatic children. The second …

Physical Therapy Alumni Rural Employment Assessment, Karen Aleyda Santos, Nikkita Jacob Crozier

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction and Purpose: The University of Nevada, Las Vegas Doctor of Physical Therapy program (UNLVPT) encourages rural and underserved employment by requiring students to complete one clinical experience in either a rural or underserved setting. Students who participate in rural clinical experiences may be more likely to practice in rural settings. UNLVPT also assesses rural interest level during each student’s education. The purpose of this study was to determine if relationship exists between students’ pre- or post-clinical experience rural interest level and their practice location within the first two years after graduation. We hypothesized that an interest in rural practice …

Comparative Expression Of Exosome-Derived Dental Pulp Stem Cell (Dpsc) Microrna, Matthew Hunsaker

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction: Dental pulp stem cells (DPSC) are pluripotent stem cells capable of differentiating into several tissue types. Several studies have demonstrated DPSC can be extracted from deciduous and permanent teeth, including third molars (wisdom teeth) - however, much less is known regarding the factors that determine and regulate pluripotency and differentiation responsiveness among these harvested DPSC. Recent evidence has suggested small, non-coding microRNAs mediate (in part) differentiation and self-renewal among some stromal and hematopoetic stem cells, although much remains to be discovered regarding microRNA regulation of DPSC phenotypes. Based upon this information, the overall goal of this study was to …

Differential Expression Of Cellular And Exosomal Microrna Isolated From Oral Cancer Cells And Their Resistance To Chemotherapy, Brady Petersen

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction: MicroRNAs are abundant small non-coding RNA with a variety of known functions, including transcriptional activation and inhibition. Recent evidence has suggested that microRNA expression may influence the responsiveness of some cancers to chemotherapy, including liver and lung cancers. Some evidence has now suggested that specific microRNAs, such as miR-21, miR-155, and miR-375, may influence oral cancer responsiveness to chemotherapy – although much remains to be discovered.

Objective: Based upon the lack of evidence in this area, the primary objective of this study was to evaluate microRNA expression and responsiveness among oral cancers.

Methods: Five commercially available oral cancer cell …

Parental Education On Human Papillomavirus Vaccination, Marguarette J. Suprien

UNLV Theses, Dissertations, Professional Papers, and Capstones

The human papillomavirus (HPV) is a worldwide condition that produces several diseases, including cervical, anogenital, and oral cancer, among others (Fishman et al., 2014). The Center for Disease Control and Prevention (CDC; 2016a) stated that HPV is among the most reported sexually transmitted diseases in the United States. Over 150 viruses are associated with HPV, and 40 types of the virus can be spread via sexual contact, including vaginal, oral, and anal sex (CDC, 2016; CDC, 2016b; CDC, 2016c). Evidence shows that approximately 79 million individuals are infected in the United States, and 14 million Americans are infected with the …

Essential Tools For Sustainability Of A Student-Led, Pro-Bono Back School, Cory Brown, Rosalie Gutierrez, Jennifer Montes, Rondale Scruggs

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background: In 2018, University of Nevada Las Vegas Doctor of Physical Therapy (DPT) students and faculty, in cooperation with a community partner, created a student-led pro bono Back School to address a gap in healthcare for underinsured individuals with low back pain in Southern Nevada. To support the sustainability of the Back School, the authors of this paper set out to develop a student club to recruit and train DPT students to volunteer in the program using a specifically designed electronic training manual. Overall, the aim was to encourage students to join the new club's leadership team to prepare future …

Regional Health Benefits Of The Nevada Renewable Energy Initiative, Alireza Rezaee

UNLV Theses, Dissertations, Professional Papers, and Capstones

In recent years, there have been tremendous efforts to replace fossil fuels with renewable energy to mitigate emissions of greenhouse gases and air pollutants in the United States. Nevada was among the first states that established the renewable portfolio standards (RPS), requiring a certain percentage of electricity sold to the customers for consumption coming from renewable resources. The recent Nevada Senate Bill 358, signed by Nevada’s Governor in 2019, set a target that by 2030 not less than 50% of electricity sold in Nevada should come from renewable resources. This research investigates how this Bill could provide health benefits for …

Utilizing Emotional Intelligence To Cultivate Occupational Therapy Students' Leadership Skills, Talina Minasian

UNLV Theses, Dissertations, Professional Papers, and Capstones

Emotional intelligence (EI) education is a crucial component to facilitating professional and leadership skills. Strengthening emotional competence through training and education can support clinician success. The healthcare sector is complex and presents many challenges and emotionally demanding circumstances. Sharpening emotional intelligence skills can provide tools for students, practitioners, and leaders to effectively respond and rise above difficult situations. There is a strong need for healthcare programs to supplement their curriculums with EI learning.

This doctoral project explored the benefits and impact of integrating emotional intelligence education into a leadership course for entry-level occupational therapy (OT) students. Using current literature, a …

Assessment Of Oral Human Papillomavirus In Pediatric And Adult Patients Within A Multi-Ethnic Clinic Population, Melissa Kornhaber

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction: Human papillomavirus (HPV) encompasses a large family of oncogenic viruses responsible for increasing rates of both cervical and oral cancer, particularly among minority and low-income populations. Although this represents an increasingly significant public health risk, few studies have screened for oral HPV within Nevada. Based upon this information, the primary objective of this study was to provide a temporal analysis of oral HPV screening among a primarily low-income, minority patient population.

Methods: This retrospective analysis was reviewed and approved by the Institutional Review Board (IRB). In brief, unstimulated saliva samples were previously obtained from clinical patient volunteers that provided …

How Long-Term, Non-Monogamous Romantic Relationships Maintain Sexual Satisfaction, Anna Kessler

UNLV Theses, Dissertations, Professional Papers, and Capstones

Eroticism is an important element of couple's relationships. Sexual intimacy has the power to bring couples closer together, as higher levels of sexual intimacy results in higher relationship satisfaction, or hinder relationships if either or both partners aren’t sexually satisfied in their relationship. The current study’s purpose is to understand how long-term, non-monogamous relationships have successfully maintained “eroticism” throughout the course of their relationship. In this study, 24 couples who are in a committed, romantic relationship of at least 7 years were interviewed. The interviews were conducted individually over a video call platform, and each was completed withing approximately 60 …

Quality Assurance Of Service Learning Back School, Eric Bashaw, Vu Pham, Tyler Parvin, Ryan Pauly

UNLV Theses, Dissertations, Professional Papers, and Capstones

Project Execution: Back School: During the 2019-20 academic year, two University of Nevada, Las Vegas Physical Therapy (UNLVPT) core faculty members, along with eight doctor of physical therapy students, provided three student-led, pro-bono back school classes at Volunteers in Medicine of Southern Nevada (VMSN). Each back school class consisted of two, 2-hour sessions where participants were screened for red flags, educated on pain management strategies and common causes of back pain, and given an individual home exercise program. Quality Assurance Surveys: Patient’s and VMSN staff were all given questions in the form of Likert-scale and open-ended questions via printed handouts …

Examining The Experiences Of Lgbtq Dpt Students: A Critical Ethnography, Catherine Turner

UNLV Theses, Dissertations, Professional Papers, and Capstones

The purpose of this study was to examine the experiences of LGBTQ Doctor of Physical Therapy (DPT) students at a large public university in the south-western United States, as it relates to the culture and climate of the program and campus. The LGBTQ community faces its own unique health disparities. Research in medicine suggests improving the diversity of the healthcare profession is a vital component in improving access to care, and this requires the creation of inclusive and supportive educational environments for LGBTQ identifying healthcare students. However, minimal research exists examining the experiences of LGBTQ physical therapy students.

The study …

Implementation Of A Protocol In The Freestanding Emergency Department To Screen For And Identify Possible Victims Of Human Trafficking, Joe Alvarado

UNLV Theses, Dissertations, Professional Papers, and Capstones

Human trafficking (HT) victims often seek medical care in the emergency room setting. In a recent study among HT survivors, 88% attested to receiving medical care while in captivity, with 63% treated in the emergency care setting. Freestanding emergency departments typically have lower acuity and lower patient volumes than hospital-based emergency departments. This setting creates shorter wait times and minimizes the length of stay at every point during the emergency room experience. For traffickers, this is an ideal situation if their victim needs medical attention, and they want to get in and get out of the emergency room with less …

Scardovia Wiggsiae And Streptococcus Sobrinus Prevalence Among Orthodontic And Non-Orthodontic Patients, Melissa Trumbo

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background: Dental cavities or caries have been identified as among the most prevalent of preventable oral conditions. However, studies are discovering new information regarding the incidence and prevalence of several cariogenic organisms, including Streptococcus mutans (SM), the recently discovered Scardovia wiggsiae (SW), as well as Streptococcus sobrinus (SS). These studies have revealed varying prevalence among different populations, such as those undergoing orthodontic treatment. Based upon this information, the main goal of the current study was to assess the prevalence of specific cariogenic organisms (SS and SW) within saliva samples originally obtained from a dental school-based clinic.

Methods: The protocol for …

Opioid And Naloxone Training In A Rural School District, Allyson C. Waldron

UNLV Theses, Dissertations, Professional Papers, and Capstones

The opioid epidemic is a national health emergency in the United States, with over 128 people dying each day due to an opioid overdose. It is estimated that 4.3 million people over the age of 12 use opioids without a medical need, and 1.3 million youth ages 12-17 use opioids inappropriately. Drug overdose deaths have become the leading cause of accidental death in the United States, prompting the U.S. Surgeon General and the World Health Organization to advise that more Americans have access to naloxone and understand how to use this life-saving drug correctly. Naloxone is an opioid antagonist, used …

Assessment Of The Infrazygomatic Crest And Distal Infrazygomatic Crest For Bone Depth And Miniscrew Insertion, Kyle C. Tuttle

UNLV Theses, Dissertations, Professional Papers, and Capstones

The aim of this study was to analyze and compare the bone depth at various postulated miniscrew insertion angles in both the Infrazygomatic Crest (IZC) and Distal Infrazygomatic Crest (DIZC) regions. Furthermore, root morphologies of the maxillary 1st and 2nd molars and their effect on bone depth and the various miniscrew insertion angles were analyzed.

A total of 69 CBCT images were collected from pre-orthodontic adult patients at the UNLV orthodontic residency clinic. Bone depth measurements were made at 8 postulated miniscrew insertion angles starting at 40° up to 75°. All measurements were recorded from the buccal bone at the …

Mapping Nevada's Dental Workforce, Kelvin Chen

UNLV Theses, Dissertations, Professional Papers, and Capstones

Access to care is a concern at the forefront of public health. Due to socioeconomic and geopolitical pressures, the distribution of healthcare providers across a population often does not coincide with the demand for healthcare in a specific geographic area. Rural areas typically do not have enough providers and urban areas typically have too many. This stark reality underscores an inherent inefficiency in the allocation of healthcare resources and is a discrepancy that must be addressed by state-sponsored institutions and programs. From a public health perspective, the problem of insufficient or lack of access to care is the greater of …

Understanding The Role Of Ano1 In Oral Cancer, Mallary Forrest

UNLV Theses, Dissertations, Professional Papers, and Capstones

In 2008, the gene ANO1 was discovered to encode a calcium activated chloride channel. This gene is located on the 11q13 locus, a locus that is commonly amplified in many cancers including cancer of the head and neck. ANO1 is situated in close proximity to genes associated with growth and apoptosis. As rapid proliferation and lack of apoptosis are hallmark characteristics of cancer, growth factors and apoptosis mediators are expected to be altered in cancer. But what does a calcium activated chloride channel have to contribute to cancer’s pathogenesis? Is it an active gene in cancer progression or is it …

Gardasil Vaccine Trends Within Nevada, California, And The U.S.: A Comparative Study, Karen S. Gutierrez

UNLV Theses, Dissertations, Professional Papers, and Capstones

Despite decreasing incidence in cervical cancer in the U.S., there continues to be an increase in public health concern for cervical cancer worldwide. Recent studies report that individuals are disproportionately affected based on region, sex, and race. Additionally, the human papillomavirus (HPV) attributable cancers may be reduced between 70% and 90% through the universal use of HPV-vaccines. In order to expand current knowledge and implement intervention programs in Nevada, it is critical to examine the associations among the Gardasil vaccine, cervical cancer screening, and adverse events following immunization as well as to understand the different socio-demographic subgroups affected. To our …

Patient And Facility Perceptions On The Feasibility Of A Student-Run Pro-Bono Back-School For Uninsured Patients With Low Back Pain, Javier Ascanio-Pellon, Sofia Cruz, Lauren Liebert, Trevor Pacuk

UNLV Theses, Dissertations, Professional Papers, and Capstones

A. Background of Knowledge: Nevada ranks 8th highest in uninsured population in the United States. People who are uninsured may be more affected by low back pain (LBP) than their insured counterparts. Volunteers in Medicine of Southern Nevada (VMSN), a non-profit organization, serves uninsured people in Nevada. VMSN partnered with UNLV Physical Therapy (UNLVPT) to provide a back-school for patients. UNLVPT proposed a service-learning model to provide both education and treatment for VMSN patients with LBP. B. Purpose: The purpose of this community-based research project is to create and implement a student-run pro-bono back-school for uninsured individuals with LBP in …