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Full-Text Articles in Medicine and Health Sciences
Bridged Filaments Of Histone-Like Nucleoid Structuring Protein Pause Rna Polymerase And Aid Termination In Bacteria., Matthew V. Kotlajich, Daniel R. Hron, Beth A. Boudreau, Zhiqiang Sun, Yuri L. Lyubchenko, Robert Landick
Bridged Filaments Of Histone-Like Nucleoid Structuring Protein Pause Rna Polymerase And Aid Termination In Bacteria., Matthew V. Kotlajich, Daniel R. Hron, Beth A. Boudreau, Zhiqiang Sun, Yuri L. Lyubchenko, Robert Landick
Journal Articles: Pharmaceutical Sciences
Bacterial H-NS forms nucleoprotein filaments that spread on DNA and bridge distant DNA sites. H-NS filaments co-localize with sites of Rho-dependent termination in Escherichia coli, but their direct effects on transcriptional pausing and termination are untested. In this study, we report that bridged H-NS filaments strongly increase pausing by E. coli RNA polymerase at a subset of pause sites with high potential for backtracking. Bridged but not linear H-NS filaments promoted Rho-dependent termination by increasing pause dwell times and the kinetic window for Rho action. By observing single H-NS filaments and elongating RNA polymerase molecules using atomic force microscopy, we …
Triplet Repeat Dna Structures And Human Genetic Disease: Dynamic Mutations From Dynamic Dna., Richard R. Sinden, Vladimir N. Potaman, Elena A. Oussatcheva, Christopher E. Pearson, Yuri L. Lyubchenko, Luda S. Shlyakhtenko
Triplet Repeat Dna Structures And Human Genetic Disease: Dynamic Mutations From Dynamic Dna., Richard R. Sinden, Vladimir N. Potaman, Elena A. Oussatcheva, Christopher E. Pearson, Yuri L. Lyubchenko, Luda S. Shlyakhtenko
Journal Articles: Pharmaceutical Sciences
Fourteen genetic neurodegenerative diseases and three fragile sites have been associated with the expansion of (CTG)n (CAG)n, (CGG)n (CCG)n, or (GAA)n (TTC)n repeat tracts. Different models have been proposed for the expansion of triplet repeats, most of which presume the formation of alternative DNA structures in repeat tracts. One of the most likely structures, slipped strand DNA, may stably and reproducibly form within triplet repeat sequences. The propensity to form slipped strand DNA is proportional to the length and homogeneity of the repeat tract. The remarkable stability of slipped strand DNA may, in part, be due to loop-loop interactions facilitated …