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Expression Of Biologically Active Human Butyrylcholinesterase In The Cabbage Looper (Trichoplusia Ni), Peter L. Platteborze, Clarence A. Broomfield
Expression Of Biologically Active Human Butyrylcholinesterase In The Cabbage Looper (Trichoplusia Ni), Peter L. Platteborze, Clarence A. Broomfield
US Army Research
This investigation examined the utility of three recombinant protein-expression systems (COS cells, insect cells and insect larvae) to cost-effectively produce biologically active human butyrylcholinesterase (BuChE). It was determined that baculovirus-infected insect cells (Sf9 and High 5) expressed 3.5- and 8.2-fold, respectively, more active enzyme than COS-7 cells. Baculovirus-infected cabbage looper (Trichoplusia ni) insect larvae produced over 26 times more than High 5 cells ; in fact, one baculovirus-infected insect larva provided more active protein than 100 ml of insect cell culture. Analysis of the larvally expressed proteins revealed that the vast majority of BuChE expressed was inactive due to …