Medicine and Health Sciences Commons^™

Sequential Alterations In Catabolic And Anabolic Gene Expression Parallel Pathological Changes During Progression Of Monoiodoacetate-Induced Arthritis, Jin Nam, Priyangi Perera, Jie Liu, Bjoern Rath, James Deschner, Robert Gassner, Timothy A. Butterfield, Sudha Agarwal

Physical Therapy Faculty Publications

Chronic inflammation is one of the major causes of cartilage destruction in osteoarthritis. Here, we systematically analyzed the changes in gene expression associated with the progression of cartilage destruction in monoiodoacetate-induced arthritis (MIA) of the rat knee. Sprague Dawley female rats were given intra-articular injection of monoiodoacetate in the knee. The progression of MIA was monitored macroscopically, microscopically and by micro-computed tomography. Grade 1 damage was observed by day 5 post-monoiodoacetate injection, progressively increasing to Grade 2 by day 9, and to Grade 3-3.5 by day 21. Affymetrix GeneChip was utilized to analyze the transcriptome-wide changes in gene expression, and …