Open Access. Powered by Scholars. Published by Universities.®
- Discipline
Articles 1 - 2 of 2
Full-Text Articles in Medicine and Health Sciences
Use Of An Alpha-Smooth Muscle Actin (Smaa) Gfp Reporter To Identify An Osteoprogenitor Population, Zana Kalajzic, Haitao Li, Li-Ping Wang, Xi Jiang, Katie B. Lamothe, Douglas J. Adams, Hector L. Aguila, David W. Rowe, Ivo Kalajzic
Use Of An Alpha-Smooth Muscle Actin (Smaa) Gfp Reporter To Identify An Osteoprogenitor Population, Zana Kalajzic, Haitao Li, Li-Ping Wang, Xi Jiang, Katie B. Lamothe, Douglas J. Adams, Hector L. Aguila, David W. Rowe, Ivo Kalajzic
UCHC Articles - Research
Identification of a reliable marker of skeletal precursor cells within calcified and soft tissues remains a major challenge for the field. To address this, we used a transgenic model in which osteoblasts can be eliminated by pharmacological treatment. Following osteoblast ablation a dramatic increase in a population of α-smooth muscle actin (α-SMA) positive cells was observed. During early recovery phase from ablation we have detected cells with the simultaneous expression of SMAA and a preosteoblastic 3.6GFP marker, indicating the potential for transition of α-SMA+ cells towards osteoprogenitor lineage. Utilizing α-SMAGFP transgene, α-SMAGFP+ positive cells were detected in the …
Osteopenia In Transgenic Mice With Osteoblast-Targeted Expression Of The Inducible Camp Early Repressor, Taranpreet K. Chandhoke, Yu-Feng Huang, Fei Liu, Gloria A. Gronowicz, Douglas J. Adams, John R. Harrison, Barbara E. Kream
Osteopenia In Transgenic Mice With Osteoblast-Targeted Expression Of The Inducible Camp Early Repressor, Taranpreet K. Chandhoke, Yu-Feng Huang, Fei Liu, Gloria A. Gronowicz, Douglas J. Adams, John R. Harrison, Barbara E. Kream
UCHC Articles - Research
ICER is a member of the CREM family of basic leucine zipper transcription factors that acts as a dominant negative regulator of gene transcription. Four different isoforms of ICER (I, Iγ, II and IIγ) are transcribed from the P2 promoter of the Crem gene. We previously found that each of the ICER isoforms is induced by parathyroid hormone in osteoblasts. The goal of the present study was to assess the function of ICER in bone by overexpressing ICER in osteoblasts of transgenic mice. ICER I and ICER II cDNAs, each containing an N-terminal FLAG epitope tag, were cloned downstream of …