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Full-Text Articles in Medicine and Health Sciences
T Cell Migration From Inflamed Skin To Draining Lymph Nodes Requires Intralymphatic Crawling Supported By Icam-1/Lfa-1 Interactions., Alvaro Teijeira, Morgan C. Hunter, Erica Russo, Steven T Proulx, Thomas Frei, Gudrun F. Debes, Marc Coles, Ignacio Melero, Michael Detmar, Ana Rouzaut, Cornelia Halin
T Cell Migration From Inflamed Skin To Draining Lymph Nodes Requires Intralymphatic Crawling Supported By Icam-1/Lfa-1 Interactions., Alvaro Teijeira, Morgan C. Hunter, Erica Russo, Steven T Proulx, Thomas Frei, Gudrun F. Debes, Marc Coles, Ignacio Melero, Michael Detmar, Ana Rouzaut, Cornelia Halin
Department of Microbiology and Immunology Faculty Papers
T cells are the most abundant cell type found in afferent lymph, but their migration through lymphatic vessels (LVs) remains poorly understood. Performing intravital microscopy in the murine skin, we imaged T cell migration through afferent LVs in vivo. T cells entered into and actively migrated within lymphatic capillaries but were passively transported in contractile collecting vessels. Intralymphatic T cell number and motility were increased during contact-hypersensitivity-induced inflammation and dependent on ICAM-1/LFA-1 interactions. In vitro, blockade of endothelial cell-expressed ICAM-1 reduced T cell adhesion, crawling, and transmigration across lymphatic endothelium and decreased T cell advancement from capillaries into lymphatic collectors …