Medicine and Health Sciences Commons^™

Investigation Of Dna Damage And Genomic Organization In The Cellular Response To Platinum Chemotherapy, Abdulhadi Abdulwahed

FIU Electronic Theses and Dissertations

Ovarian cancer (OC) is the primary reason for gynecological cancer-related deaths among women. This cancer rarely has specific symptoms making early detection challenging. Platinum compounds have been used as the first-line treatment for OC. However, OC patients often develop chemoresistance to current platinum/taxane chemotherapy regimens upon recurrence, leaving patients with no alternative treatment options. The purpose of this thesis was to investigate the molecular basis of chemoresistance in OC by analyzing the damage of DNA response and gene positioning after treatment exposure. A modified comet assay was used to measure interstrand cross-link (ICL) formation and repair in chemoresistant (CR) (SKOV-3) …