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Full-Text Articles in Medicine and Health Sciences
Mechanism Of Calcium Transport Stimulated By Chlorothiazide In Mouse Distal Convoluted Tubule Cells., Frank A. Gesek, Peter A. Friedman
Mechanism Of Calcium Transport Stimulated By Chlorothiazide In Mouse Distal Convoluted Tubule Cells., Frank A. Gesek, Peter A. Friedman
Dartmouth Scholarship
Thiazide diuretics inhibit Na+ and stimulate Ca2+ absorption in renal distal convoluted tubules. Experiments were performed on immortalized mouse distal convoluted tubule (MDCT) cells to determine the mechanism underlying the dissociation of sodium from calcium transport and the stimulation of calcium absorption induced by thiazide diuretics. Control rates of 22Na+ uptake averaged 272 +/- 35 nmol min-1 mg protein-1 and were inhibited 40% by chlorothiazide (CTZ, 10(-4) M). Control rates of 36Cl- uptake averaged 340 +/- 50 nmol min-1 mg protein-1 and were inhibited 50% by CTZ. CTZ stimulated 45Ca2+ uptake by 45% from resting levels of 2.86 +/- 0.26 …
Adenosine Regulates A Chloride Channel Via Protein Kinase C And A G Protein In A Rabbit Cortical Collecting Duct Cell Line., Erik M. Schwiebert, Katherine H. Karlson, Peter A. Friedman, Paul Dietl, William S. Spielman, Bruce Stanton
Adenosine Regulates A Chloride Channel Via Protein Kinase C And A G Protein In A Rabbit Cortical Collecting Duct Cell Line., Erik M. Schwiebert, Katherine H. Karlson, Peter A. Friedman, Paul Dietl, William S. Spielman, Bruce Stanton
Dartmouth Scholarship
We examined the regulation by adenosine of a 305-pS chloride (Cl-) channel in the apical membrane of a continuous cell line derived from rabbit cortical collecting duct (RCCT-28A) using the patch clamp technique. Stimulation of A1 adenosine receptors by N6-cyclohexyladenosine (CHA) activated the channel in cell-attached patches. Phorbol 12,13-didecanoate and 1-oleoyl 2-acetylglycerol, activators of protein kinase C (PKC), mimicked the effect of CHA, whereas the PKC inhibitor H7 blocked the action of CHA. Stimulation of A1 adenosine receptors also increased the production of diacylglycerol, an activator of PKC. Exogenous PKC added to the cytoplasmic face of inside-out patches also stimulated …