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Full-Text Articles in Medicine and Health Sciences
A Polymerase Chain Reaction-Based Method To Detect Cisplatin Adducts In Specific Genes, M M. Jennerwein, A Eastman
A Polymerase Chain Reaction-Based Method To Detect Cisplatin Adducts In Specific Genes, M M. Jennerwein, A Eastman
Dartmouth Scholarship
Every bulky lesion in DNA can potentially inhibit the Taq DNA polymerase and thereby decrease the amplification produced in the polymerase chain reaction. We investigated the feasibility of using this inhibition to quantify DNA lesions produced by the anticancer drug cisplatin. Products were detected by electrophoresis followed by ethidium bromide staining. Quantitation was obtained by including [32P]dCTP in the amplification reaction and subsequently assessing the incorporated radioactivity. Hamster genomic DNA was platinated in vitro to defined levels and amplified with primers that produce either a 150, 750 or 2,000 base pair fragment. The degree of inhibition of PCR …
Neurospora Crassa Clock-Controlled Genes Are Regulated At The Level Of Transcription., Jennifer J. Loros, Jay C. Dunlap
Neurospora Crassa Clock-Controlled Genes Are Regulated At The Level Of Transcription., Jennifer J. Loros, Jay C. Dunlap
Dartmouth Scholarship
Although an extensive number of biological processes are under the daily control of the circadian biological clock, little is known about how the clock maintains its regulatory networks within a cell. An important aspect of this temporal control is the daily control of gene expression. Previously we identified two morning-specific genes that are regulated by the clock through daily control of gene expression (J. Loros, S. Denome, and J.C. Dunlap, Science 243:385-388, 1989). We have now introduced a method for transcriptional analysis in Neurospora crassa and used this nuclear run-on procedure to show that regulation of mRNA abundance for these …