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Full-Text Articles in Medicine and Health Sciences
Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs, Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Nuananong Sinwat, Rungtip Chuanchuen
Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs, Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Nuananong Sinwat, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
A total of 292 E. coli isolates obtained from fecal samples from pigs in Central (n = 103) and Northeastern (n = 189) provinces of Thailand were included in this study. Eighty-six E. coli isolates were phenotypically confirmed to be β- lactamases producers and screened for the presence of β-lactamase genes. The CTX-M family was most frequently identified (90.7%). The blaCTX-M-15 gene belonging to CTX-M group I (59.3%) was the most predominantly identified CTX-M genotype followed by blaCTX-M-14 (31.4%) and blaCTX-M-4 (25.6%). The blaTEM-1 gene was prevalent (75.6%). The blaCTX-M-4 and blaCTX-M-14 genes were located on conjugative plasmid. The results …
Identification Of Genotype And Phenotype Of Antimicrobial Resistance Of Escherichia Coli Isolates From Pigs In Southern Vietnam, Do Tien Duy, Nguyen Luong Lam Anh, Nguyen Thi Kim Thoa, Phitsanuwattana K, Thongratsakul S, Carrique-Mas Jj, Le Thanh Hien, Nguyen Thi Thu Nam, Nguyen Tat Toan
Identification Of Genotype And Phenotype Of Antimicrobial Resistance Of Escherichia Coli Isolates From Pigs In Southern Vietnam, Do Tien Duy, Nguyen Luong Lam Anh, Nguyen Thi Kim Thoa, Phitsanuwattana K, Thongratsakul S, Carrique-Mas Jj, Le Thanh Hien, Nguyen Thi Thu Nam, Nguyen Tat Toan
The Thai Journal of Veterinary Medicine
Escherichia coli is a primary reservoir of antimicrobial resistance, known chiefly for the container of AMR-encoding genes (ARGs), and poses potential risks to human and animal health. This study investigated AMR phenotypes and ARGs in 90 E. coli isolates from different pig groups in 10 farms in southern Vietnam. The minimum inhibitory concentration (MIC) of 19 common antimicrobial agents was determined, and polymerase chain reaction (PCR) was used to investigate seven ARGs (blaTEM, aadA1, strA, dfrA12, sul3, cmlA and tetA). Cohen’s kappa statistic (κ) was applied to assess the concordance between phenotypic and genotypic profiles. A total of 81.1% of …
Nasal Colonization Of Pigs And Farm Attendants By Staphylococcus Aureus And Methicillin-Resistant Staphylococcus Aureus (Mrsa) In Kebbi, Northwestern Nigeria, Mohammed Sani Gaddafi, Yusuf Yakubu, Abdulkadir Usman Junaidu, Mohammad Bashir Bello, Bashiru Garba, Asinamai Athliamai Bitrus, Habiba Lawal
Nasal Colonization Of Pigs And Farm Attendants By Staphylococcus Aureus And Methicillin-Resistant Staphylococcus Aureus (Mrsa) In Kebbi, Northwestern Nigeria, Mohammed Sani Gaddafi, Yusuf Yakubu, Abdulkadir Usman Junaidu, Mohammad Bashir Bello, Bashiru Garba, Asinamai Athliamai Bitrus, Habiba Lawal
The Thai Journal of Veterinary Medicine
Methicillin Resistant Staphylococcus aureus (MRSA) is a leading cause of nosocomial, community and livestockassociated colonization and infection worldwide. This study aimed at investigating the nasal colonization of pigs and farm attendants by S. aureus and MRSA in Kebbi, North-Western Nigeria. A cross-sectional study was conducted in Kebbi Northwestern Nigeria using simple random sampling. A total of 212 nasal swabs were collected from two farms consisting of 100 samples each from pigs and 12 samples from farm attendants. A well-structured questionnaire was administered for risk factor analysis. Nasal swabs were examined using routine bacteriological culture and identification. Presumptive MRSA was confirmed …
Testicular Gene Expression Of Anti-Müllerian Hormone, Androgen Receptor And Inhibin Alpha Subunit In Porcine Cryptorchidism, Gunnaporn Suriyaphol, Shanaporn Leelakajornkit, Wipa Sripodok, Wirakan Kallayanathum, Arunthip Komonpunporn, Nutthee Am-In, Sirinun Pisamai, Nathamon Yimpring
Testicular Gene Expression Of Anti-Müllerian Hormone, Androgen Receptor And Inhibin Alpha Subunit In Porcine Cryptorchidism, Gunnaporn Suriyaphol, Shanaporn Leelakajornkit, Wipa Sripodok, Wirakan Kallayanathum, Arunthip Komonpunporn, Nutthee Am-In, Sirinun Pisamai, Nathamon Yimpring
The Thai Journal of Veterinary Medicine
Cryptorchidism, one of the common congenital abnormalities in boars, results ina boar taint in the carcass and economic loss. However, knowledge of gene expression of hormones controlling the testicular descent process, such as anti-Müllerian hormone (AMH), androgen receptor (AR) and inhibin alpha subunit (INHA), in porcine testicular cryptorchidismis still limited. The present study aimed to investigate the gene expression profiles of AMH, ARand INHAin undescended testes (UDT) and descended testes (DT) in unilateral cryptorchid pigs of suckling (12 weeks of age), nursery (6weeks of age) and growing-finishing pigs (1520 weeksof age) and in normal testes (NT) at 12 weeks of …
Effects Of Β-Glucan And Mannan-Oligosaccharide Supplementation On Growth Performance, Fecal Bacterial Population, And Immune Responses Of Weaned Pigs, Phan Thi Tuoi, Pornchalit Assavacheep, Kris Angkanaporn, Anongnart Assavacheep
Effects Of Β-Glucan And Mannan-Oligosaccharide Supplementation On Growth Performance, Fecal Bacterial Population, And Immune Responses Of Weaned Pigs, Phan Thi Tuoi, Pornchalit Assavacheep, Kris Angkanaporn, Anongnart Assavacheep
The Thai Journal of Veterinary Medicine
The effects of β-glucan and mannan-oligosaccharide (BG + MOS) supplementation in feed on growth performance, bacterial population in feces and immune responses of weaned pigs were investigated. Two hundred and eighty-eight weaning piglets were randomly allocated into 3 groups with 8 replicates per group and 12 pigs per replicate. Group 1, as control, was fed a commercial basal nursery diet. Groups 2 and 3 were fed the same diet supplemented with BG + MOS (Biolex® MB-40) 1 and 2 kg/ton of feed, respectively. Weight gain (WG), average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR) …
Application Of Real-Time Pcr For Quantifying Gastrointestinal Microbiota In Weaning Pigs Influenced By Dietary Feed Additive Supplementation, Phattarapon Chaloemnon, Mullika Traidej Chomnawang, Wirawan Junlapho, Nuanchan Paraksa
Application Of Real-Time Pcr For Quantifying Gastrointestinal Microbiota In Weaning Pigs Influenced By Dietary Feed Additive Supplementation, Phattarapon Chaloemnon, Mullika Traidej Chomnawang, Wirawan Junlapho, Nuanchan Paraksa
The Thai Journal of Veterinary Medicine
To apply real-time polymerase chain reaction (PCR) to detect and quantify the gastrointestinal microbiota modulated by dietary feed additives was the objective of this study. Sixty-four three-crossbred weaning piglets at 24 days were used and randomly assigned to be fed one of the experimental diets supplemented with different feed additives (0.2% inulin, 0.1% fructooligosaccharide and sub-therapeutic antibiotics), and compared with the non-supplemented group. Comparison of microbiota enumeration by conventional culture and real-time PCR methods in response to feed additives was investigated. The microbiota enumeration by conventional culture and real-time PCR methods showed corresponding responses of selected bacterial counts (Lactobacilli, Bifidobacteria, …
A Reassortant Virus Of A Thai Swine Influenza Virus (Siv) And The Pandemic H1n1 Of Pig Origin Did Not Induce Severe Disease In Experimental Ducks, Jirapat Arunorat, Nataya Charoenvisal, Juthatip Keawcharoen, Donruethai Sreta, Alongkorn Amonsin, Roongroje Thanawongnuwech
A Reassortant Virus Of A Thai Swine Influenza Virus (Siv) And The Pandemic H1n1 Of Pig Origin Did Not Induce Severe Disease In Experimental Ducks, Jirapat Arunorat, Nataya Charoenvisal, Juthatip Keawcharoen, Donruethai Sreta, Alongkorn Amonsin, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Currently, the pandemic H1N1 (pH1N1) 2009 influenza A virus and its reassortant pandemic H1N1 influenza (rH1N1) viruses have been circulating in pigs population in many countries around the world including Thailand. Many organizations and researchers have raised some concerns about interspecies transmission, particularly in aquatic avian species including domestic ducks commingling in backyard farming. In order to elucidate the pathogenesis of those viruses in domestic ducks, the study on pH1N1and rH1N1 recently isolated from pigs was conducted to understand the susceptibility, clinical signs, viral shedding and lesions in experimental ducks. Twenty-one 6-week-old influenza A virus negative ducks were divided into …
Probable Pig To Duck Transmission Of The Pandemic H1n1 2009 (Ph1n1) And Its Reassortant In Commingling Experimental Condition, Nataya Charoenvisal, Juthatip Keawcharoen, Donruethai Sreta, Siriporn Tantawet, Suphattra Jittimanee, Jirapat Arunorat, Korakrit Poonsuk, Alongkorn Amonsin, Roongroje Thanawongnuwech
Probable Pig To Duck Transmission Of The Pandemic H1n1 2009 (Ph1n1) And Its Reassortant In Commingling Experimental Condition, Nataya Charoenvisal, Juthatip Keawcharoen, Donruethai Sreta, Siriporn Tantawet, Suphattra Jittimanee, Jirapat Arunorat, Korakrit Poonsuk, Alongkorn Amonsin, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Pandemic H1N1 2009 (pH1N1) virus is considered as a low pathogenic influenza virus, however, it rapidly spread among humans and was finally found in the swine population of 6 continents. Interspecies transmissions among different animal species are of interest. In this study, sentinel ducks were commingled with pH1N1 or pandemic H1N1 reassortment virus (rH1N1) inoculated pigs in separate groups. According to the results, both studied viruses were able to cross-species transmit to a few sentinel ducks with mild or no clinical signs. Viral shedding measured by a modified real time RT-PCR detection from the oropharyngeal and cloacal swabs were also …
Genetic Characterization And Phylogenetic Analysis Of Porcine Circovirus Type 2 In Thai Pigs With Porcine Circovirus Associated Diseases (Pcvad) During 2007-2010, Suphattra Jittimanee, Suparlark Nuntawan Na Ayudhya, Roongtham Kedkovid, Komkrich Teankum, Roongroje Thanawongnuwech
Genetic Characterization And Phylogenetic Analysis Of Porcine Circovirus Type 2 In Thai Pigs With Porcine Circovirus Associated Diseases (Pcvad) During 2007-2010, Suphattra Jittimanee, Suparlark Nuntawan Na Ayudhya, Roongtham Kedkovid, Komkrich Teankum, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Porcine circovirus type 2 (PCV2) was first recognized as a causative agent of post weaning multisystemicsyndrome or PMWS in Canada in 1991. Subsequently, it has been reported in almost all intensive pig productioncountries, and currently all associated diseases linked to PCV2 are included under the term porcine circovirusassociated diseases or PCVAD. PCV2 can be divided into 2 major genotypes referred to genotype 1 (PCV2b) andgenotype 2 (PCV2a). In Thailand, PMWS was first reported in 1998. However, only one isolate of Thai PCV2 from ourgroup has been submitted to GenBank (AY864814). Therefore, the objective of this study was to determine thegenetic …
Genetic Characterization Of Porcine Epidemic Diarrhea Virus (Pedv) Isolates From Southern Vietnam During 2009-2010 Outbreaks, Do Tien Duy, Tat Toan Nguyen, Suphasawatt Puranaveja, Roongroje Thanawongnuwech
Genetic Characterization Of Porcine Epidemic Diarrhea Virus (Pedv) Isolates From Southern Vietnam During 2009-2010 Outbreaks, Do Tien Duy, Tat Toan Nguyen, Suphasawatt Puranaveja, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Porcine epidemic diarrhea virus (PEDV) spike (S) glyprotein and membrane (M) glycoprotein genes arebelieved to have genetic variation. The heterogeneity in those genomic sequences has been reported and is knownessentially for the diverse PEDV pathogenicity. Eight southern Vietnamese PEDVs collected from severe waterydiarrhea piglets of the recent emerging outbreaks (2009-2010) were sequenced and analyzed. The results revealedhigh nucleotide homology of the partial S gene of the current isolates at 98.9-100% and 99.7-100% identity of the fullM gene among these isolates despite dividing into two subclusters of different provincial origins. It should be notedthat the Vietnamese PEDVs contained high differences on …
Culex Tritaeniorhynchus Is Unlikely To Be A Vector For The Porcine Reproductive And Respiratory Syndrome Virus (Prrsv), Kidsadagon Pringproa, Sudchit Chungpivat, Rapee Panyathong, Roongroje Thanawongnuwech
Culex Tritaeniorhynchus Is Unlikely To Be A Vector For The Porcine Reproductive And Respiratory Syndrome Virus (Prrsv), Kidsadagon Pringproa, Sudchit Chungpivat, Rapee Panyathong, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
At least, 4 mosquito species, Culex tritaeniorhynchus, Culex gelidus, Anopheles vagus and Mansonia uniformis were identified on a pig farm in Nakorn Pathom, Thailand between March 2004 to February 2005. C. tritaeniorhynchus was found to be the predominant species (88.387.63%). To determine whether C. tritaeniorhynchus could serve as a mechanical vector for PRRSV transmission, 2 experiments were conducted on: the duration of PRRSV within the mosquitoes and PRRSV transmission from the PRRSV-infected pigs to naive pigs by the infected mosquitoes. PRRSV nucleic acid could be detected in the whole pooled mosquito samples for up to 48 hours post feeding in …
The Detection Of Ifn-Γ Positive Cells In The Lymphoid Tissues Of Prrsv-Infected Pigs Using Different Strains, For Infection, Tuddow Thaiwong, Chanis Leetheraprasert, Chaiwat Winyoonunthakul, Anudep Rungsipipat, Supradit Wangnaitham, Roongroje Thanawongnuwech
The Detection Of Ifn-Γ Positive Cells In The Lymphoid Tissues Of Prrsv-Infected Pigs Using Different Strains, For Infection, Tuddow Thaiwong, Chanis Leetheraprasert, Chaiwat Winyoonunthakul, Anudep Rungsipipat, Supradit Wangnaitham, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Archived paraffin-embedded lymphoid tissue samples from seventy five experimental PRRSV-infected pigs were examined for PRRSV antigen and IFN-γ positive cells, using immunohistochemistry. Lymphoid tissues (5 pigs/group) including spleen, tracheobronchial lymph nodes and tonsil came from negative control pigs (group 1), low virulence strain of PRRSV (RespPRRS/ReproTM, modified-live virus vaccine) infected pigs (group 2) and high virulence strain of PRRSV (VR-2385) infected pigs(group 3). They were necropsied at 3, 7,10, 14 or 28 days post-inoculation (DPI). According to the study, microscopic lesions in the lymphoid tissues from pigs infected with the high virulence strain had more severe lesions, characterized by severe …
Cytokine Profiles Following Mycoplasma Hyopneumoniae And Prrsv Co-Infection In Pigs, Roongroje Thanawongnuwech, Eileen L. Thacker
Cytokine Profiles Following Mycoplasma Hyopneumoniae And Prrsv Co-Infection In Pigs, Roongroje Thanawongnuwech, Eileen L. Thacker
The Thai Journal of Veterinary Medicine
Bronchoalveolar cells collected by lung lavage 28 days post infection (DPI) of negative controls, Mycoplasma hyopneumoniae (M. hyo)-infected, Porcine Respiratory and Reproductive Syndrome virus (PRRSV)-infected and dual-infected pigs, were evaluated for various cytokine mRNA (IL1α, IL1β, IL6, IL8, IL10, IL12, and TNFα). Semiquantitative RT-PCR revealed that IL1β, IL6, IL10 and the IL12 mRNA expression was significantly increased in dual-infected pigs, compared with the negative controls. PRRSV-infected pigs had significantly increased levels of IL10 and IL12 mRNA. The increased IL10 gene expression that was observed in the dual infected pigs may play a role in the potentiation of PRRSV-induced pneumonia by …
Antibiotic Susceptibility Of Pigs To E. Coli F4+, Pornchalit Assavacheep, Anongnart Somchit, Apisida Srikrauedong, Nipaporn Paiboonkijkul
Antibiotic Susceptibility Of Pigs To E. Coli F4+, Pornchalit Assavacheep, Anongnart Somchit, Apisida Srikrauedong, Nipaporn Paiboonkijkul
The Thai Journal of Veterinary Medicine
The prevalence and antibiotic sensitivity of E. coli., and haemolytic E. coli. F4+ and haemolytic E. coli. F4- (E/F4+, E/F4-, HE/F4+ and HE/F4-) was investigated in five hundred and thirty, randomly collected, rectal swabs from pig herds in five areas of Thailand, between the dates June 2000 and January 2001. The prevalence of E/F4+, in each area was statistically significantly different with a range of 21.8-42.5% (30.4±8.8%). The greatest prevalence was found in the Northern area of Thailand, following by the Central, Western, Eastern, and Northeastern areas, respectively. The prevalence of E. coli. and haemolytic E. coli. F4+ and F4- …
Pulmonnary Intravascular Macrophages (Pims): What Do We Know About Their Role In Prrsv Infection In Pigs?, Roongroje Thanawongnuwech
Pulmonnary Intravascular Macrophages (Pims): What Do We Know About Their Role In Prrsv Infection In Pigs?, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Porcine pulmonary intravascular macrophages (PIMs) have been recently known for their role of pulmonary surveillance. In the past few years, we have been investigating the role of PIMs in porcine reproductive and respiratory syndrome virus (PRRSV) infection. PRRSV antigen has been demonstrated in PIMs both in vitro and in vivo. PRRSV-infected PIMs reveal viral particles that tend to accumulate in the smoothed-wall vesicles and yield a high virus titer. The PRRSV infection induces either apoptosis or cell lysis. The bactericidal activity of in vitro PRRSV infected PIMs is significantly decreased and the phagocytic activity is questionable as the pulmonary copper …
Endogenous Amino Acid Losses In Pigs And Poultry: A Review(การสูญเสียกรดอะมิโนภายในระบบทางเดินอาหารของไก่และสุกร : บทความ), Kris Angkanaporn, Wayne L. Bryden, Velmurugu Ravindran
Endogenous Amino Acid Losses In Pigs And Poultry: A Review(การสูญเสียกรดอะมิโนภายในระบบทางเดินอาหารของไก่และสุกร : บทความ), Kris Angkanaporn, Wayne L. Bryden, Velmurugu Ravindran
The Thai Journal of Veterinary Medicine
Considerable quantities of non-dietary protein are secreted into the digesta of pigs and poultry. This protein is defined as the protein found in the chyme or faeces when a nitrogen-free diet is fed to animals. Endogenous proteins comprise protein, peptides, amino acids and nitrogen-containing substances from saliva, bile, pancreatic, gastric and intestinal secretions; plasma and sloughed epithelial cells. These endogenous proteins are digested and absorbed in a manner similar to those of dietary origin. Conventional approaches to the estimation of obligatory loss have included the measurement of amino acid excretion in fasted animals, in animals fed on protein-free diets, or …