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Full-Text Articles in Medicine and Health Sciences
Effect Of Daily Environment And Season In A Tropical Environment On The Expression Of Heat Shock Protein, And Steroidogenic And Apoptotic Genes In Bovine Cumulus-Oocyte Complex, S. Kanwichai, S. Panasophonkul, J. K. Bernard, W. Suriyasathaporn
Effect Of Daily Environment And Season In A Tropical Environment On The Expression Of Heat Shock Protein, And Steroidogenic And Apoptotic Genes In Bovine Cumulus-Oocyte Complex, S. Kanwichai, S. Panasophonkul, J. K. Bernard, W. Suriyasathaporn
The Thai Journal of Veterinary Medicine
The objectives of this study were to determine the differences in the expression of heat shock protein, and steroidogenic and apoptosis genes by cumulus-oocyte complex (COC) of beef cattle in a tropical environment as affected by seasonal and day effect of temperature and relative humidity. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to measure gene expression in COC in different seasons including cool (Jan to Feb), summer (Mar to Apr) and rainy (May to Jul) seasons. Ambient temperature and relative humidity recorded on the day before oocyte collection were used to classify environment groups as: low temperature and low …
Effect Of Nicotine On Gene Expression And Developmental Competence Of Bovine Oocytes, Seree Teerapong, Tassanee Faisaikarm, Natakarn Nimsanor, Chinarat Changsangfa, Kornkanok Promthep, Kulnasan Saikhun
Effect Of Nicotine On Gene Expression And Developmental Competence Of Bovine Oocytes, Seree Teerapong, Tassanee Faisaikarm, Natakarn Nimsanor, Chinarat Changsangfa, Kornkanok Promthep, Kulnasan Saikhun
The Thai Journal of Veterinary Medicine
Nicotine affected meiosis during oocyte maturation is associated with poor subsequent embryonic development. The aim of this study was to analyze the effects of nicotine exposure during in vitro maturation (IVM) on the outcomes of in vitro fertilization (IVF) and the expression of gene related development of oocytes and embryos. Immature (germinal vesicle, GV) oocytes were randomly cultured in IVM media containing nicotine at concentrations of 0.0 (control) 2.5, 5.0, and 10.0 mM. After culturing for 24 h, oocytes were assessed for their maturation rate, subjected to IVF and mRNA extraction for gene expression analysis using quantitative real time PCR …
Cathelicidin Responded To Streptococcus Agalactiae And Associated With The Severity Of Subclinical Mastitis, Pongphol Pongthaisong, Suporn Katawatin, Chaiyapas Thamrongyoswittayakul
Cathelicidin Responded To Streptococcus Agalactiae And Associated With The Severity Of Subclinical Mastitis, Pongphol Pongthaisong, Suporn Katawatin, Chaiyapas Thamrongyoswittayakul
The Thai Journal of Veterinary Medicine
Cathelicidin in milk was reported as a possible protein marker for the assessment of the severity of subclinical mastitis. We hypothesized that cathelicidin would be elevated in the mastitic milk of the Streptococcus agalactiae-subclinical mastitis and the elevation of cathelicidin may be related to somatic cell count (SCC). Western blot was used to determine the amount of cathelicidin to differentiate between normal milk and S. agalactiae subclinical mastitic milk. The correlation between amount of cathelicidin and the SCC was also determined. We found that S. agalactiae subclinical mastitis induced cathelicidin expression in milk. There was a significant correlation between the …
In Vitro Modulatory Effects Of Quercetin On Bovine Neutrophil Effector Functions, Phongsakorn Chuammitri, Chiradet Amphaiphan, Panupong Nojit
In Vitro Modulatory Effects Of Quercetin On Bovine Neutrophil Effector Functions, Phongsakorn Chuammitri, Chiradet Amphaiphan, Panupong Nojit
The Thai Journal of Veterinary Medicine
Bovine neutrophils perform numerous effector functions to overcome microbial invasions by utilizing innately-equipped intracellular and extracellular killing mechanisms. In this study, we explored the modulatory effects of quercetin hydrate (QH), an herbal flavonoid compound, on bovine neutrophil functional activities. Isolated neutrophils were incubated with various concentrations of QH (0-100 mM). Neutrophil viability results showed no signs of cytotoxicity (p = 0.33). In other assays, neutrophils were stimulated with a pre-defined concentration of QH (50 mM). Results showed promising effects of QH on enhancing intracellular ROS generation (p < 0.0001) compared to control. Phagocytic activity of fluorescently labeled E.coli was also augmented by the effects of this compound (p = 0.05). However, intracellular killing of live E.coli quantified by MTT assay demonstrated a slight decrease in activity (p = 0.304). Additionally, extracellular killing driven by NET-DNA release was remarkably higher in QH-stimulated cells (p = 0.0004). Supplementation of QH to LPS stimulated neutrophils resulted in significantly decreased pro-inflammatory cytokines, IL-1b (p < 0.0001) and TNF-a gene expression (p = 0.004). Taken together, quercetin could exert its noteworthy actions, both at cellular and molecular levels, at a certain physiological concentration.
In Vitro Development Potentiality Of Expanded Bovine Blastocysts Subsequent To Cryotop Vitrification, Ashit Kumar Paul, Yuanyuan Liang, Takashi Nagai, Rangsun Parnpai
In Vitro Development Potentiality Of Expanded Bovine Blastocysts Subsequent To Cryotop Vitrification, Ashit Kumar Paul, Yuanyuan Liang, Takashi Nagai, Rangsun Parnpai
The Thai Journal of Veterinary Medicine
Embryo cryopreservation is a promising area of study in the field of reproductive research. One of the issues that have recently arisen is the discovery that the growing ability of embryos after cryopreservation varies depending on the culture period of the embryo prior to its cryopreservation. Therefore, the present study was designed to explore the in vitro development potentiality of bovine embryos at day 7 and day 8, as well as their survivability and hatchability after Cryotop vitrification. The blastocyst rate at day 8 (28.1%) was higher than that of day 7 (19.1%). Grade 1 (G1) and grade 2 (G2) …
Expression Patterns Of Cell Adhesion Molecules In Bovine Preimplantation Embryos Cultured In Vitro, Anucha Sathanawongs, Korakot Nganvongpanit, Supamit Mekchay
Expression Patterns Of Cell Adhesion Molecules In Bovine Preimplantation Embryos Cultured In Vitro, Anucha Sathanawongs, Korakot Nganvongpanit, Supamit Mekchay
The Thai Journal of Veterinary Medicine
This study aimed to investigate gene expression patterns throughout preimplantation embryos and their comparative expression in morphological quality blastocysts using real-time RT-PCR. Bovine oocytes underwent maturation process, fertilized and were cultured in vitro. Degree of fragmentation and differentiation were the criteria to categorize day-7 blastocysts as of good and poor quality. All of the embryos from each developmental stage were used to quantify transcript abundance. It was found that E-cadherin was highly expressed in immature and mature oocytes, then it decreased at 2- to 16-cell stages, and later increased in morula and blastocyst stages. The β-catenin transcript was highly abundant …
First Identification Of Neospora Caninum In Thailand, Than Kyaw, Prachin Virakul, Manop Muangyai, Wijit Banlunara
First Identification Of Neospora Caninum In Thailand, Than Kyaw, Prachin Virakul, Manop Muangyai, Wijit Banlunara
The Thai Journal of Veterinary Medicine
An immunohistochemical examination of aborted bovine tissues, collected from two dairy farms that had a high abortion rate (7%), detected the organism Neospora caninum (NC). Five out of 12 aborting cows (41.7%) were seropositive to NC using a competitive ELISA test. Neospora-tachyzoites were detected in the placenta of a seropositive aborting cow. Neither NC parasites nor cysts were detected in other fetal tissues. The identification of the Neospora-parasite and a high percentage of Neospora seropositive aborting cows, suggested NC as the possible cause of abortion. This is the first identification of the NC parasite in Thailand.
The Presence Of Sialic Acid In Bovine Serum Amyloid P Component (กรดไซอะลิคในซีรั่มโปรตีน Amyloid P Component ในโค), Meena Sarikaputi, Masaharu Naiki
The Presence Of Sialic Acid In Bovine Serum Amyloid P Component (กรดไซอะลิคในซีรั่มโปรตีน Amyloid P Component ในโค), Meena Sarikaputi, Masaharu Naiki
The Thai Journal of Veterinary Medicine
Neuraminidase-treated bovine serum amyloid P component (SAP) has chromatographic profile from DEAE-HPLC column of shorter retention time and less peak number than intact molecule does. SDS-PAGE analysis reveals a decrease in size of bovine SAP subunits after neuraminidase treatment, compared with an untreated molecule. This study indicates a presence of sialic acid in bovine SAP.(จากการวิเคราะห์ซีรั่มโปรตีน amyloid P component (SAP) ในโคด้วยเครื่อง High performance liquid chromatography (HPLC) โดยใช้คอลัมภ์ DEAE พบว่า ซีรั่มโปรตีน SAP ที่ผ่านการย่อยด้วยเอนไซม์ neuraminidase มี retention time สั้นกว่า และจำนวน peak ของโปรตีนน้อยกว่าซีรั่มโปรตีนชนิดเดียวกันที่ไม่ได้ผ่านการย่อย จากการ วิเคราะห์ด้วยอิเลคโตรฟอเรซีสบนเจล SDS-PAFE พบว่าหน่วยย่อยของซีรั่มโปรตีน SAP ที่ผ่านการย่อยด้วยเอนไซม์ neutraminidase มีขนาดเล็กลงเมื่อเทียบกับซีรั่มโปรตีนชนิดเดียว กันในสภาพธรรมชาติ ผลการวิเคราะห์โดยวิธีดังกล่าวแสดงให้เห็นว่ามีกรดไซอะลิคเป็น ส่วนประกอบอยู่ในโมเลกุลของซีรั่มโปรตีน SAP ในโค)
Environmental Effects On The Quality Of Bovine Semen(ผลของสภาวะแวดล้อมต่อคุณภาพของน้ำเชื้อโคและกระบือ), M. Subhan Quereshi, H.A. Samad, Zabita Khan, Shafgat Hussain
Environmental Effects On The Quality Of Bovine Semen(ผลของสภาวะแวดล้อมต่อคุณภาพของน้ำเชื้อโคและกระบือ), M. Subhan Quereshi, H.A. Samad, Zabita Khan, Shafgat Hussain
The Thai Journal of Veterinary Medicine
The interaction between semen quality of Holstein Friesian, Jersey, Crossbred and Nili-Ravi buffaloes and the environment was studied. Semen was collected for one year at the Semen Production Unit, Surizai District, Peshawar from 10 Holstein Friesian, 4 Jersey and 4 Crossbred bulls and 3 buffaloes. Semen was collected early in the morning, twice weekly, using an artificial vagina. Environmental data regarding the monthly average temperature, humidity and daylight, was recorded. The correlation co-effi-cient between the semen volume, motility and sperm concentration and the three environmental parameters was calculated. Statistical analysis showed that in buffalo bulls motility was significantly (r> 0.5874) …
Complete Sequence Of Bovine C-Reactive Protein Complementary Dna, Meena Sarikaputi, Masami Morimatsu, Masayki Saito, Masaharu Naiki
Complete Sequence Of Bovine C-Reactive Protein Complementary Dna, Meena Sarikaputi, Masami Morimatsu, Masayki Saito, Masaharu Naiki
The Thai Journal of Veterinary Medicine
Bovine CRP cDNA of 2.2 kb was isolated from bovine liver cDNA library by using the probe containing sequence homologous in human CRP and rabbit CRP. Restriction map of this cDNA showed only one site for each following restriction enzyme EcoRI, HindIII, PstI, Sacl and Xbal. DNA sequence analysis revealed that bovine CRP cDNA has 57 bp coded for signal peptide of 19 amino acid and 618 bp for mature protein of 206 amino acid residues. The first and the last amino acid residues of the mature protein, deduced from its DNA sequence was found to be glutamine and tryptophan, …
Production Of Monoclonal Antibodies Against Bovine C-Reactive Protein, M. Sarikaputi, W. Nunomura, M. Morimatsu, M. Naiki
Production Of Monoclonal Antibodies Against Bovine C-Reactive Protein, M. Sarikaputi, W. Nunomura, M. Morimatsu, M. Naiki
The Thai Journal of Veterinary Medicine
Three monoclonal antibodies (MAbs) against bovine C-reactive protein (CRP) were produced by fusing murine myeloma cell line P3X63Ag8U1 and splenic lympocytes from two BALB/c mice immunized with 100 ug of purified CRP, followed by two booster injections at 10-day intervals. The hybridomas producing antibodies specific for bovine CRP were obtained with effciencies of 1.3 % and 0.2 % respectively. All three MAbs were IgM class with k- light chains. The MAbs obtained from ascitic fluid showed definite antibody activity at 1,000 to 10,000 and the antibody concentrations were 0.9 to 1.7 mg/ml. Two of three MAbs showed significant cross-reactivity with …