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Articles 1 - 30 of 94
Full-Text Articles in Medicine and Health Sciences
Safety And Toxicity Evaluation Of Bronopol In Striped Catfish (Pangasianodon Hypophthalmus), Patharapol Piamsomboon, Mintra Lukkana, Janenuj Wongtavatchai
Safety And Toxicity Evaluation Of Bronopol In Striped Catfish (Pangasianodon Hypophthalmus), Patharapol Piamsomboon, Mintra Lukkana, Janenuj Wongtavatchai
The Thai Journal of Veterinary Medicine
The study was performed to evaluate the safety and toxicity of bronopol in striped catfish Pangasianodon hypophthalmus. Bronopol product is licensed in Thailand and Vietnam for control of aquatic fungi and ciliated protozoa in scaleless fish at the daily dosage of 10 mg/l, 2-hour static immersion, for 5 consecutive days. Safe concentrations were evaluated in fish exposed to bronopol at the therapeutic dosage (10 mg/l, 2 hours, 5 consecutive days) and 2 and 3 times overdose (20 and 30 mg/l, 2 hours, 5 consecutive days). Fish treated with 10-30 mg/l bronopol for 2 hours daily for 5 consecutive days appeared …
An In Vitro Comparative Study Of Aflatoxin B1 Adsorption By Thai Clay And Commercial Toxin Binders, Bundit Tengjaroenkul, Urai Tengjaroenkul, Natapol Pumipuntu3, Komkrich Pimpukdee, Sawitree Wongtangtintan, Piyawat Saipan
An In Vitro Comparative Study Of Aflatoxin B1 Adsorption By Thai Clay And Commercial Toxin Binders, Bundit Tengjaroenkul, Urai Tengjaroenkul, Natapol Pumipuntu3, Komkrich Pimpukdee, Sawitree Wongtangtintan, Piyawat Saipan
The Thai Journal of Veterinary Medicine
Twenty samples of Thai clay from fourteen provinces and seven commercial toxin binders were investigated for their adsorption capacity of aflatoxin B1 (AFB1) in vitro. Each sample of 5 mg/l AFB1 solution was shaken at 250C for 24 hours and supernatants of centrifuged samples were analyzed for concentrations of AFB1 using a UV spectrophotometer. Adsorption capacity was calculated and applied to isothermic equations. The results indicated that Thai clays was capable of sequestering AFB1 from aqueous solution differently and Thai clay from Lopburi and Lamphun provinces had the highest adsorption capacity, similar to commercial binders. S-shaped isothermic curves were observed …
Delayed Post-Ovulatory Progesterone Rise In Thai-Holstein Dairy Heifers: Association With Endocrine Events Around Oestrus, Effects On Conception Rate And Possibility For Progesterone Supplement Post-Insemination, Sudsaijai Kornmatitsuk, Bunlue Kornmatitsuk, Yindee Kitiyanant
Delayed Post-Ovulatory Progesterone Rise In Thai-Holstein Dairy Heifers: Association With Endocrine Events Around Oestrus, Effects On Conception Rate And Possibility For Progesterone Supplement Post-Insemination, Sudsaijai Kornmatitsuk, Bunlue Kornmatitsuk, Yindee Kitiyanant
The Thai Journal of Veterinary Medicine
The current research aimed to further study the delayed post-ovulatory progesterone (P4) rise in Thai-Holstein dairy heifers in the points of an association with endocrine events around oestrus (experiment 1), effects on conception rate and possibility for P4 supplement post-insemination (experiment 2). In experiment 1, the P4 levels in the delayed P4 heifers reached 1 ng/ml later than in the normal ones (6.8±0.4 vs 3.3±1.5 days after oestrus, resp.) (p = 0.053). At ovulation the pre-ovulatory follicles of the delayed P4 were smaller than of the normal P4 heifers (11.8±0.5 vs 13.1±0.2 mm, resp.) (p = 0.013). The levels of …
Comparison Of Nutrient Contents, Digestibility And Fecal Output Of Concentrate Rations (Gel) For Dog, Uttra Jamikorn
Comparison Of Nutrient Contents, Digestibility And Fecal Output Of Concentrate Rations (Gel) For Dog, Uttra Jamikorn
The Thai Journal of Veterinary Medicine
The purposes of this current study were to compare the amount of nutrient contents and digestibility between different concentrate ration brands and their effects on fecal output. These concentrate rations mean the diets formulated in order to improve the nutritive status of the animals. In this study, nine mature female mongrel dogs were randomly subjected into three groups of the triplicated 3x3 Latin square design. Three brands of concentrate ration in form of gel were used as dietary treatments. Nutrient contents (on DM basis), body weight, fecal output, and apparent digestibility of nutrients were determined. Percentages of dry matter, organic …
Determination Of Antimicrobial Susceptibility, Antimicrobial Resistance Genes And In Vivo Testing Of Antimicrobial Susceptibility Of Avibacterium Paragallinarum, Tippayaporn Noonkhokhetkong, Kridda Chukiatsiri, Jiroj Sasipreeyajan, Niwat Chansiripornchai
Determination Of Antimicrobial Susceptibility, Antimicrobial Resistance Genes And In Vivo Testing Of Antimicrobial Susceptibility Of Avibacterium Paragallinarum, Tippayaporn Noonkhokhetkong, Kridda Chukiatsiri, Jiroj Sasipreeyajan, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
Avibacterium paragallinarum is the causative agent of infectious coryza, an acute upper respiratory disease in chickens. The aim of this study was to characterize the antimicrobial susceptibility, resistance genes and the in vivo testing of antimicrobial susceptibility in chickens. Eighteen A. paragallinarum isolated in Thailand were tested for antimicrobial susceptibility by broth microdilution method. A high prevalence of resistance MIC pattern to oxytetracycline, doxycycline, streptomycin, ciprofloxacin, erythromycin and sulfamethoxazole-trimethoprim was found. Thirteen isolates showed multiple antimicrobial drug resistance (66.7%). Resistance genes of tet(A), tet(B), tet(M), erm(A), erm(B), blaROB-1 and sul2 were found and the resistance genes of tet(A), tet(B) and …
Pathogenicity And Transmission Of Thai Hpai-H5n1 In Japanese Quails, Thawat Lekdumrongsak, Wijit Banlunara, Rachod Tantilertcharoen, Nutthawan Nonthabenjawan, Komkiew Pinpimai, Jiroj Sasipreeyajan, Alongkorn Amonsin
Pathogenicity And Transmission Of Thai Hpai-H5n1 In Japanese Quails, Thawat Lekdumrongsak, Wijit Banlunara, Rachod Tantilertcharoen, Nutthawan Nonthabenjawan, Komkiew Pinpimai, Jiroj Sasipreeyajan, Alongkorn Amonsin
The Thai Journal of Veterinary Medicine
Pathogenicity and transmission of Highly Pathogenic Avian Influenza subtype H5N1 viruses (HPAI-H5N1) depend on the strains and infection doses of the viruses. In this study, experimental infection was performed to determine pathogenicity and transmission of the Thai HPAI-H5N1 in Japanese quails. One hundred and seven quails were divided into 4 groups, experimental groups 1-3 (n = 32 per group) and negative control group (n = 11). At the age of 9 weeks, 16 quails each from groups 1-3 were inoculated with 107, 105 and 103 EID50 of Thai HPAI-H5N1, A/chicken/Thailand/CUK2/04(CU-K2) virus. Twenty-four hours later, 16 quails were added as contact …
Milk Production And Mammary Extraction Of Nutrients By Late Lactating Cross-Bred Saanen Goats Supplemented With Betaine In Diet, Nungnuch Saipin, Somchai Chanpongsang, Narongsak Chaiyabutr
Milk Production And Mammary Extraction Of Nutrients By Late Lactating Cross-Bred Saanen Goats Supplemented With Betaine In Diet, Nungnuch Saipin, Somchai Chanpongsang, Narongsak Chaiyabutr
The Thai Journal of Veterinary Medicine
The objective of this study was to investigate the effect of supplementary betaine in diet on milk secretion, milk composition, mammary extraction of nutrients and relevant parameters in late lactating cross-bred Saanen goats. Ten, multiparous, non-pregnant cross-bred Saanen goats of approximately 10 weeks postpartum were used for the study. The animals were divided into two groups of five animals each. Animals in the experimental group received diet containing 4 g betaine per kg of concentrate diet for four weeks, while the control group received similar concentrate diet without betaine as concurrent control. Results showed that milk yields of betaine supplemented …
Enhancing Specific Antibodies Using A Developed Sub-Unit Pcv2b Vaccination In A Pcv2-Affacted Herd, Suphattra Jittimanee, Suparlark Nuntawan Na Ayudhya, Roongtham Kedkovid, Komkrich Teankum, Sanipa Suradhat, Roongroje Thanawongnuwech
Enhancing Specific Antibodies Using A Developed Sub-Unit Pcv2b Vaccination In A Pcv2-Affacted Herd, Suphattra Jittimanee, Suparlark Nuntawan Na Ayudhya, Roongtham Kedkovid, Komkrich Teankum, Sanipa Suradhat, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Porcine circovirus type 2 (PCV2) causes multisystemic diseases called porcine circovirus associated disease (PCVAD) in pigs leading to a significant economic loss in the swine industry. The objective of this study was to determine the efficacy of a developed sub-unit PCV2b vaccine using a recombinant truncated capsid protein expressed in Escherichia coli in PCVAD-affected pig farms. Five-week-old conventional pigs were allocated into 2 groups: vaccinated (n = 55) and control (n = 55) groups placed in the same building. The vaccinated pigs were given two shots of a developed vaccine with 2-week interval on day 0 and 14. Serum samples …
Critical Factors On Chemical Properties And Heavy Metals In Water For Livestock Farms In Thailand, Teeraporn Makaroon, Suwicha Kasemsuwan, Nattavut Ratanavanichrojn, Hathairad Hananantachai, Rachaneekorn Mingkhwan, Sutha Khaodhiar, Phitsanu Tulayakul
Critical Factors On Chemical Properties And Heavy Metals In Water For Livestock Farms In Thailand, Teeraporn Makaroon, Suwicha Kasemsuwan, Nattavut Ratanavanichrojn, Hathairad Hananantachai, Rachaneekorn Mingkhwan, Sutha Khaodhiar, Phitsanu Tulayakul
The Thai Journal of Veterinary Medicine
This study aimed to investigate chemical quality of animal drinking used in livestock farms in Thailand. Samples and information from 68 pig farms, 35 chicken farms and 9 duckling farms were collected during March to August 2011. Water quality data were analyzed and compared with standard levels using the Mann-Whitney U and Wilcoxon Signed Rank Tests. Results revealed that median chemical values of water for livestock were generally within standard levels, except manganese and iron, which were detected in surfacewater with median values of 0.183 and 0.506 ppm, respectively, and are both higher than standard limits for drinking water. Median …
Clastogenic And Anticlastogenic Potential Of Neem Flower Extract Evaluated By Rat Liver Micronucleus Assay, Piengchai Kupradinun, Yaninee Jarratwisarutporn, Rawiwan Wongpoomchai, Hathaitip Sritanaudomchai, Mati Rienkitjakarn
Clastogenic And Anticlastogenic Potential Of Neem Flower Extract Evaluated By Rat Liver Micronucleus Assay, Piengchai Kupradinun, Yaninee Jarratwisarutporn, Rawiwan Wongpoomchai, Hathaitip Sritanaudomchai, Mati Rienkitjakarn
The Thai Journal of Veterinary Medicine
Clastogenic and anticlastogenic effects of neem flower (Azadirachta indica A. Juss) extract were determined using a rat liver micronucleus assay. Methanol extracts of neem flowers (MENF) in 15% Tween 80 at 100 and 500 mg/kg body weight were orally given to male Sprague Dawley rats daily for 6 weeks (2 weeks prior to and 4 weeks during repeated administration of diethylnitrosamine (DEN). All rats were anesthetized and their livers were collected. Hepatocytes were isolated from the livers using a collagenase solution without performing liver perfusion, then stained with 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI). Incidence of the micronucleated hepatocytes was evaluated by …
Analysis Of Mirna-29a Expression In Porcine Peripheral Blood Mononuclear Cells Using Quantitative Reverse Transcription Polymerase Chain Reaction, Sunaree Nantakhruea, Porntippa Lekcharoensuk, Win Surachetpong
Analysis Of Mirna-29a Expression In Porcine Peripheral Blood Mononuclear Cells Using Quantitative Reverse Transcription Polymerase Chain Reaction, Sunaree Nantakhruea, Porntippa Lekcharoensuk, Win Surachetpong
The Thai Journal of Veterinary Medicine
MicroRNAs (miRNAs) are small non coding RNA that regulate gene expression as post transcriptional regulators in mammalian tissues. One of the miRNAs that participates in mammalian immune response is miR-29a. Despite the limited information of miR-29a in porcine cells, it is interesting to determine whether miR-29a involves in immune regulation in pig. In this study, we developed a quantitative reverse transcription polymerase chain reaction using SYBR detection system to examine the expression of primary miR-29a (pri-miR-29a) in porcine peripheral blood mononuclear cells (PBMCs) in the presence of mitogen. PBMCs were stimulated with concanavalin A (Con A) at a concentration of …
Field Efficacy Of Potassium Peroxymonosulfate (Pms) Oxidizing Disinfectant (Virusniptm) Against Porcine Circovirus Type 2 (Pcv2) In Gilt Acclimatization Unit, Korakrit Poonsuk, Jirapat Arunorat, Sasiwimon Talummuk, Rapeepat Kunalintip, Woravit Anuvongnukroh, Roongroje Thanawongnuwech
Field Efficacy Of Potassium Peroxymonosulfate (Pms) Oxidizing Disinfectant (Virusniptm) Against Porcine Circovirus Type 2 (Pcv2) In Gilt Acclimatization Unit, Korakrit Poonsuk, Jirapat Arunorat, Sasiwimon Talummuk, Rapeepat Kunalintip, Woravit Anuvongnukroh, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Virusnip™ is one of the commercial potassium peroxymonosulfate disinfectants widely used in swine farms. This disinfectant is well studied and proves to be excellent in antimicrobial activity previously. However, there is no information about its efficacy under field condition. Thus, the objective of this study was to determine the virucidal activity of Virusnip® against PCV2 in farm condition. Consequently, 1:200 concentration of VirusnipTM was sprayed for 15 minutes once or twice daily depending on the acclimatization period in gilts isolation units compared to the untreated group in the same building. Environmental swabs and oral fluid samples were collected and pooled …
Molecular Characterization Of A Genetically Diverse Bubaline Picobirnavirus Strain, India, Yashpal Singh Malik, Naveen Kumar, Kuldeep Sharma, Amit Kumar Sharma, Subhankar Sircar, Lalit M. Jeena, Neeraj K. Singh, Anjan Mondal, Siddhartha N. Joardar, Ganesh Balasubramanian
Molecular Characterization Of A Genetically Diverse Bubaline Picobirnavirus Strain, India, Yashpal Singh Malik, Naveen Kumar, Kuldeep Sharma, Amit Kumar Sharma, Subhankar Sircar, Lalit M. Jeena, Neeraj K. Singh, Anjan Mondal, Siddhartha N. Joardar, Ganesh Balasubramanian
The Thai Journal of Veterinary Medicine
Picobirnavirus (PBV) associated gastrointestinal infections have been noticed in humans as well as in a number of animal species, however, to the best of our information, report about the detection and characterisation of PBV in buffaloes is not presented. In this study, RNA polymerase (RdRp) gene targeted RT-PCR assay was used to recognize PBVs and additionally the isolate was analysed for evolutionary and sequence homology. The buffalo PBV isolate was of genogroup I (GG I) nature and a comparative sequence analysis revealed homology of 44.5% and 45.1% at nucleotide and amino acid levels, respectively, with the prototype human PBV strain …
Ophthalmology Snapshot, Nalinee Tuntivanich
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep, Rampaipat Tungjitpeanpong
What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep, Rampaipat Tungjitpeanpong
The Thai Journal of Veterinary Medicine
No abstract provided.
Molecular Characterization Of Chicken Infectious Anemia Virus Outbreaks During 2008-2011 In Thailand, Wisanu Wanasawaeng, Jirayu Buatong, Supawadee Chaichote, Niwat Chansiripornchai
Molecular Characterization Of Chicken Infectious Anemia Virus Outbreaks During 2008-2011 In Thailand, Wisanu Wanasawaeng, Jirayu Buatong, Supawadee Chaichote, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
The present study aimed to characterize chicken infectious anemia virus isolated from various outbreaks in Thailand during 2008-2011, and compare them with other strains from the GenBank database using molecular and bioinformatic techniques. For this purpose, thirteen samples were collected from eight commercial farms affected by chicken infectious anemia outbreak. All were positive by PCR and grouped in 2 clusters with phylogenetic analysis based on nucleotide sequencing, consistent with amino acid sequence analysis of the VP1 gene in the hypervariable region. All of the thirteen strains were closely related to Chinese and Japanese strains with a high percentage of sequence …
Efficacy And Safety Of Different Live Mycoplasma Gallisepticum Vaccines In Layer Chickens, Somsak Pakpinyo, Suwarak Wanaratana, Kreangkrai Sangthongdang, Marcelo Paniago
Efficacy And Safety Of Different Live Mycoplasma Gallisepticum Vaccines In Layer Chickens, Somsak Pakpinyo, Suwarak Wanaratana, Kreangkrai Sangthongdang, Marcelo Paniago
The Thai Journal of Veterinary Medicine
The efficacy and safety of different live Mycoplasma gallisepticum (MG) vaccines were determined. One hundred and fifty six-week-old female layer chickens were equally divided into 6 groups. Groups 1-6 received F (two groups), 6/85 and ts-11 strains, positive and negative control, respectively. At 8 weeks old, 5 birds/group were sacrificed and gross thoracic airsac lesion scores (GTALS) and microscopic tracheal lesion scores (MTLS) were blindly evaluated. At 12 weeks old, groups 1-5 were challenged through an eye dropping with approximately 1x106 CFU of MG Thai isolated. At 13 and 14 weeks old, 10 birds/group were swabbed in the palatine fissure …
Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Thanusorn Phakhawambodee
Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Thanusorn Phakhawambodee
The Thai Journal of Veterinary Medicine
No abstract provided.
Ultrasound Diagnosis, Phiwipha Kamonrat
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
Molecular Characterization Of Respiratory Bacterial Pathogens In Large And Small Ruminants, Gemerlyn Gonzales Garcia, Lawrence Pascual Belotindos, Claro Niegos Mingala
Molecular Characterization Of Respiratory Bacterial Pathogens In Large And Small Ruminants, Gemerlyn Gonzales Garcia, Lawrence Pascual Belotindos, Claro Niegos Mingala
The Thai Journal of Veterinary Medicine
Bacterial isolates from different cases of respiratory infections in small and large ruminants were evaluated using bacterial culture and semi-nested PCR and identification was confirmed by DNA sequencing. Universal primers targeting the bacterial 16S rRNA for bacteria were used. Other sets of primers that were specific for Gram-positive bacteria and combinations of primers specific for Gram-negative organisms were used in the second PCR. The amplified PCR products were subjected to DNA sequence analysis. The DNA sequences of the isolated bacteria were aligned with the DNA sequences of bacteria in the GenBank through BLAST. Results confirmed isolation of three Gram-positive and …
Molecular Characterization Of Wenyonella Philiplevinei From Ducks In Hunan Province, China, Hui Lan Wu, Peng Hui Hu, Jin Zhang
Molecular Characterization Of Wenyonella Philiplevinei From Ducks In Hunan Province, China, Hui Lan Wu, Peng Hui Hu, Jin Zhang
The Thai Journal of Veterinary Medicine
Wenyonella philiplevinei is one of the important pathogens of duck coccidiasis. In the present study, a portion of the 18S rDNA was amplified from W. philiplevinei by Polymerase chain reaction (PCR), cloned and then sequenced. The sequences of 18S rDNA of all samples were 422 bp in size. A + T content of the 18S rDNA sequences was 58-59%. Sequence comparison revealed that the similarity in 18S rDNA sequences between Hunan isolates and W. philiplevinei available (Guangdong isolate) was more than 98%. Intra-specific sequence variations within each of the Hunan isolates were 0-1.7%. However, the inter-specific sequence differences between W. …
Testicular Atrophy And Its Related Changes In Culled Boars: A Pathological Investigation, Komkrich Teankum, Padet Tummaruk, Sawang Kesdangsakonwut, Thiti Antarasena, Sithichok Lacharoj, Jinda Singlor, Annop Kunavongkrit, Roongroje Thanawongnuwech
Testicular Atrophy And Its Related Changes In Culled Boars: A Pathological Investigation, Komkrich Teankum, Padet Tummaruk, Sawang Kesdangsakonwut, Thiti Antarasena, Sithichok Lacharoj, Jinda Singlor, Annop Kunavongkrit, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Testicular atrophy is a major cause of culling in boars. Pathogenesis of this change is poorly understood. Therefore, we aimed to investigate pathological changes of the genital tracts of culled boars with testicular atrophy. Twenty-eight genital organs of culled boars collected from slaughter houses in Thailand were pathologically examined. According to testicular weight, the organs were allocated into 4 groups: severe atrophy (n = 12), moderate atrophy (n = 4), mild atrophy (n = 4), and normal control (n = 8). In the severe atrophic group, the mean weight of atrophic testes (175.1 ± 51 g) was lower than those …
Screening And Characterization Of Lactic Acid Bacteria From Animal Faeces For Probiotic Properties, Tanatip Thamacharoensuk, Nuttha Thongchul, Malai Taweechotipatr, Vasana Tolieng, Kentaro Kodama, Somboon Tanasupawat
Screening And Characterization Of Lactic Acid Bacteria From Animal Faeces For Probiotic Properties, Tanatip Thamacharoensuk, Nuttha Thongchul, Malai Taweechotipatr, Vasana Tolieng, Kentaro Kodama, Somboon Tanasupawat
The Thai Journal of Veterinary Medicine
Twenty-two bacterial isolates from wild elephant faeces and 4 isolates from buffalo faeces in Thailand were investigated for probiotic properties and their lactic acid production. On the basis of their phenotypic characteristic clustering and 16S rRNA gene sequence analysis, the isolates were divided into seven groups. Group I (10 isolates) was identified as Enterococcus hirae, group II (4 isolates) as E. avium, group III (3 isolates) as Pediococcus pentosaceus , IV (3 isoaltes) as P. acidilactici, group V (3 isolates) as Lactobacillus pentosus, group VI (1 isolate) as Lactococcus garvieae and group VII (2 isolates) as Weissella paramesenteroides. All of …
Bone Cell Function: A Review, Hoai Nam Nguyen, Naruepon Kampa
Bone Cell Function: A Review, Hoai Nam Nguyen, Naruepon Kampa
The Thai Journal of Veterinary Medicine
Bone is dynamic tissue which is continuously formed and absorbed by its own cells in response to stimuli such as hormones, mechanical loading and growth factors. Understanding the function of bone cells is important, not only in orthopedic field but also in research study involving bone. Bone cells work in harmony to maintain a balance between bone formation and resorption, ultimately to control bone structure and function. Osteoblasts are cells, which contribute to deposition of organic components of bone extracellular matrix. They control recruitment, differentiation and maturation of osteoclasts that participate in resorption activity. In addition, osteoclasts associated with bone …
Acute Pulmonary Toxicity Caused By Single Intratracheal Instillation Of Various Doses Of Colloidal Silver Nanoparticles In Mice: Pathological Changes, Particle Bioaccumulation And Metallothionien Protein Expression, Theerayuth Kaewamatawong, Wijit Banlunara, Pattwat Maneewattanapinyo, Chuchaat Thammacharoen, Sanong Ekgasit
Acute Pulmonary Toxicity Caused By Single Intratracheal Instillation Of Various Doses Of Colloidal Silver Nanoparticles In Mice: Pathological Changes, Particle Bioaccumulation And Metallothionien Protein Expression, Theerayuth Kaewamatawong, Wijit Banlunara, Pattwat Maneewattanapinyo, Chuchaat Thammacharoen, Sanong Ekgasit
The Thai Journal of Veterinary Medicine
To study acute lung toxicity of various doses of colloidal silver nanoparticles (Ag-NPs), mice were intratracheally instilled with 0, 10, 100, 1000 or 10,000 ppm of Ag-NPs. Histopathology, autometallography (AMG) and immunohistochemistry were determined at 1, 3, 7 and 15 days post-exposure.Instillation of 100, 1,000 and 10,000 ppm Ag-NPs produced moderate to severe necrotizing bronchitis and alveolitiswith hypertrophy and hyperplasia of alveolar epithelial cells. The severity of the pulmonary inflammation and damage increased in a dose-dependent manner. Concomitant lamininimmunohistochemical findings generally correlated with pulmonary lesions. Interleukin 1- beta (IL-1β) and tumor necrotic factor-alpha (TNF-α) positive immunostaining were found in the …
Efficacy Of Different Vaccination Programs Against Velogenic Newcastle Disease Virus Challenged In 28-Day-Old Broiler Chickens, Tawatchai Pohuang, Nida Sirikobkul, Jiroj Sasipreeyajan
Efficacy Of Different Vaccination Programs Against Velogenic Newcastle Disease Virus Challenged In 28-Day-Old Broiler Chickens, Tawatchai Pohuang, Nida Sirikobkul, Jiroj Sasipreeyajan
The Thai Journal of Veterinary Medicine
In the present study, the efficacy of different vaccination programs against a velogenic Newcastle disease virus (vNDV) challenged in 28-day-old broiler chickens was examined. The chickens were divided into 5 groups, 20 chickens in each. Group 1 did not receive any vaccine and served as the non-vaccinated control group. Groups 2-5 were vaccinated with different vaccination programs against vNDV. At 1-day-old, the chickens in all the vaccinated groups were vaccinated with live Newcastle disease (ND) vaccine, Ulster 2C strain, followed by different inactivated vaccines including vaccine A in groups 2 and 4 and vaccine B in groups 3 and 5. …
Inactivation Of Infectious Bronchitis Virus With Various Kinds Of Disinfectants, Pheemphat Bengtong, Thotsapol Thomrongsuwannakij, Niwat Chansiripornchai
Inactivation Of Infectious Bronchitis Virus With Various Kinds Of Disinfectants, Pheemphat Bengtong, Thotsapol Thomrongsuwannakij, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
This study aimed to perform an in vitro testing of the efficacy of various kinds of disinfectant on infectious bronchitis virus (IBV). Four kinds of disinfectants including Virusnip, Omnicide, CID2000 and Virkon S were used for the virus inactivation test. After challenge with each strain of IBV for each contact time (30 seconds, 1, 5 and 30 minutes), we recorded number of dead embryo/6 inoculated eggs after incubation. Results showed that there was no significant difference among the contact times except 1:800 Virusnip tested for Tha07 (p < 0.05). For Tha03, there was a significant difference among the disinfectants at 30 seconds and 1 minute of contact times (p < 0.05). For Tha08, there was a significant difference among the disinfectants at 1 minute of contact time (p < 0.05). For Tha09, there was a significant difference among the disinfectants at 5 and 30 minutes of contact times (p < 0.05). For Tha10, there was a significant difference among the disinfectants at 5 minutes of contact time (p < 0.05). Virusnip revealed the ability to inactivate the activity of 9 IBV strains in all exposure times, especially at dilutions of 1 : 100 and 1 : 200.
Evaluation Of Hematology Profiles And Measurement Of Serum Cardiac Troponin Level In Canine Monocytic Ehrlichiosis, Rungrote Osathanon, Walasinee Moonarmart, Naiyana Suksantilap, Nattiya Krajangpit, Piyanart Lekcharoensook, Pruksa Julapanthong, Nutthakulporn Wongrerkngam
Evaluation Of Hematology Profiles And Measurement Of Serum Cardiac Troponin Level In Canine Monocytic Ehrlichiosis, Rungrote Osathanon, Walasinee Moonarmart, Naiyana Suksantilap, Nattiya Krajangpit, Piyanart Lekcharoensook, Pruksa Julapanthong, Nutthakulporn Wongrerkngam
The Thai Journal of Veterinary Medicine
Canine monocytic ehrlichiosis (CME) is an important multisystemic disease in dogs which is caused by Ehrlichia canis (E. canis). Systemic inflammatory response syndrome (SIRS) and severe anemia from E. canis might be the potential causes of myocardial damage. Cardiac troponin T (cTnT) is considered to be a sensitive and specific biomarker for myocardial damage in many mammalian species. The objectives of this study were to evaluate the effect of CME on serum cTnT levels in dogs and to determine the relationship between serum cTnT and values obtained from hematology. Fifty-two client-owned dogs were recruited into this study and clinical data …
Viability And Growth Of Preantral Follicles Derived From Cryopreserved Ovarian Tissues Of A Cheetah (Acinonyx Jubatus) Post-Mortem, Grisnarong Wongbandue, Nae Tanpradit, Daraka Thongthainun, Paweena Thuwanut, Kaywalee Chatdarong
Viability And Growth Of Preantral Follicles Derived From Cryopreserved Ovarian Tissues Of A Cheetah (Acinonyx Jubatus) Post-Mortem, Grisnarong Wongbandue, Nae Tanpradit, Daraka Thongthainun, Paweena Thuwanut, Kaywalee Chatdarong
The Thai Journal of Veterinary Medicine
This study aimed to investigate freezing effects of ovarian tissues on survival of preantral follicles and observing in vitro growing of preantral follicles retrieved from cryopreserved ovarian cortical tissues of a cheetah post-mortem. After 29-hour cold storage, ovarian cortices were cut into small pieces (2.0 x 2.0 x 1.0 mm3) and allocated to be frozen using a passive cooling container (n = 3 pieces) or vitrification (n=3 pieces). After one year of storage, 43 (10/23) and 21% (12/58) follicles isolated from ovarian tissues cryopreserved using a passive cooling device (slow freezing rate) and vitrification, respectively, were viable (positively stained with …
Endoscopic Evaluation Of Gastric Mucosa To Determine Safety Of Three Chondroprotective Drugs In Healthy Dogs, Korakot Nganvongpanit, Kittipong Kungprathum, Terdsak Yano, Kumpanart Soontornvipart
Endoscopic Evaluation Of Gastric Mucosa To Determine Safety Of Three Chondroprotective Drugs In Healthy Dogs, Korakot Nganvongpanit, Kittipong Kungprathum, Terdsak Yano, Kumpanart Soontornvipart
The Thai Journal of Veterinary Medicine
This randomized, double-blind, placebo-controlled study investigated the effects of glucosamine/chondroitin sulfate, chondroitin sulfate, and diacerein on the gastric mucosa of dogs, and on clinical signs of vomiting and diarrhea. Twenty-one healthy adult dogs were included in the study, and were randomly assigned to seven treatment groups, 3 dogs per group. The effect of those medicines on the gastric mucosa was evaluated by endoscopy on day 3 and day 14, and compared with pre-treatment. Clinical signs, including vomiting and diarrhea, were recorded every day during the study period. The results showed a non-significant effect of glucosamine/chondroitin sulfate, chondroitin sulfate, and diacerein …