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Full-Text Articles in Medicine and Health Sciences
The Effect Of Luteolin On Human Glioblastoma, David M. Anson, Samson Amos, Robert L. Paris, Denise S. Simpson
The Effect Of Luteolin On Human Glioblastoma, David M. Anson, Samson Amos, Robert L. Paris, Denise S. Simpson
The Research and Scholarship Symposium (2013-2019)
Glioblastoma multiforme (GBM) is widely recognized as the most common and lethal of the malignant gliomas. Few effective therapeutic treatments are available as five-year survival rates of diagnosed individuals are less than five percent. Luteolin, a common flavonoid found in a variety of fruits and vegetables, has demonstrated significant promise in combating cancers of the breast, colon, liver, lung, and bone. In this study, we investigated the effects of luteolin on glioblastoma multiforme cell lines U-251, U-87, and U-1242. Cell viability was assessed using cell count with trypan blue exclusion and MTT assays. Results revealed that luteolin reduces GBM cell …
Validation Of Humanized Mouse Antibodies, Meiling G. Norfolk, Rocco J. Rotello
Validation Of Humanized Mouse Antibodies, Meiling G. Norfolk, Rocco J. Rotello
The Research and Scholarship Symposium (2013-2019)
Antibody therapy is being developed and tested as one of the most promising agents for treatment of various human diseases. As of March 2016, 350 antibody candidates are in clinical trials. Many of these antibodies have been taken from animals and “humanized” by genetic modification. Our experiment tests monoclonal antibodies that have been harvested from mouse hybridoma (spleen-derived) cells and cloned until the heavy and light chains of the antibody can be recognized by human cells. Because of this “humanization” procedure, basic antibody assays are needed to demonstrate that the binding, specificity and functional parameters of the antibodies are not …
Are Cell Death Proteins/Antigens Found On Interdigital Cells Dying During Limb Development Expressed In A Simple Organism Such As Tetrahymena?, Rocco J. Rotello, Jessica A. Ward, Samuel Franklin, Jenna G. Lawhead
Are Cell Death Proteins/Antigens Found On Interdigital Cells Dying During Limb Development Expressed In A Simple Organism Such As Tetrahymena?, Rocco J. Rotello, Jessica A. Ward, Samuel Franklin, Jenna G. Lawhead
The Research and Scholarship Symposium (2013-2019)
Numerous studies have been published that describe the genes and proteins that control cell death in various biological systems including normal embryonic development and in disease such as cancer. We describe attempts to look at a possible conserved cell death antigen in the simple organism Tetrahymena, using a unique monoclonal antibody that recognizes only dying cells in the chick limb. The main impetus for the research is to answer the question; does the cell death process have key proteins that exist in the dying process that can be modulated prior to the completion of the cell death process? Using various …