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Australian Catholic University

Skeletal muscle

Musculoskeletal System

Publication Year

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Full-Text Articles in Medicine and Health Sciences

No Evidence Of Direct Association Between Glut4 And Glycogen In Human Skeletal Muscle, Robyn M. Murphy, Marcelo Flores-Opazo, Barnaby P. Frankish, Andrew Garnham, David Stapleton, Mark Hargreaves Jan 2018

No Evidence Of Direct Association Between Glut4 And Glycogen In Human Skeletal Muscle, Robyn M. Murphy, Marcelo Flores-Opazo, Barnaby P. Frankish, Andrew Garnham, David Stapleton, Mark Hargreaves

Faculty of Health Sciences Publications

Previous studies have demonstrated that exercise increases whole body and skeletal muscle insulin sensitivity that is linked with increased GLUT4 at the plasma membrane following insulin stimulation and associated with muscle glycogen depletion. To assess the potential direct association between muscle glycogen and GLUT4, seven untrained, male subjects exercised for 60 min at ~75% VO2 peak, with muscle samples obtained by percutaneous needle biopsy immediately before and after exercise. Exercise reduced muscle glycogen content by ~43%. An ultracentrifugation protocol resulted in a ~2‐3‐fold enriched glycogen fraction from muscle samples for analysis. Total GLUT4 content was unaltered by exercise ...


Striated Muscle Activator Of Rho Signalling (Stars) Is Reduced In Ageing Human Skeletal Muscle And Targeted By Mir-628-5p, Andrew Russell, M. A. Wallace, M. Kalanon, Evelyn Zacharewicz, P. A.D. Gatta, Andrew Garnham, Séverine Lamon Jan 2017

Striated Muscle Activator Of Rho Signalling (Stars) Is Reduced In Ageing Human Skeletal Muscle And Targeted By Mir-628-5p, Andrew Russell, M. A. Wallace, M. Kalanon, Evelyn Zacharewicz, P. A.D. Gatta, Andrew Garnham, Séverine Lamon

Faculty of Health Sciences Publications

Aim: The striated muscle activator of Rho signalling (STARS) is a muscle-specific actin-binding protein. The STARS signalling pathway is activated by resistance exercise and is anticipated to play a role in signal mechan-otransduction. Animal studies have reported a negative regulation of STARS signalling with age, but such regulation has not been investigated in humans. Methods: Ten young (18–30 years) and 10 older (60–75 years) subjects completed an acute bout of resistance exercise. Gene and protein expres-sion of members of the STARS signalling pathway and miRNA expression of a subset of miRNAs, predicted or known to target members of ...