Medicine and Health Sciences Commons^™

Non-Opsonic Attachment Of Bordetella Bronchiseptica Mediated By Cd11/Cd18 And Cell Surface Carbohydrates, Karen B. Register, Mark R. Ackermann, Marcus E. Kehrli Jr.

Mark R. Ackermann

Porcine atrophic rhinitis associated with Bordetella bronchiseptica is characterized by a severe inflammatory response in the mucosa of the nasal turbinates. Initial infiltrates of polymorphonuclear leukocytes (PMN) are followed by accumulations of mononuclear cells. In this report, we have investigated the interaction between porcine PMN and B. bronchiseptica. PMN incubated in PBS with a fluorescently labeled hemagglutinating porcine isolate, but not a non-hemagglutinating variant, had high levels of cell-associated fluorescence as determined by flow cytometry. Light microscopy indicated that most cell-associated bacteria were ingested. Transmission electron microscopy confirmed the presence of intracellular bacteria, which were contained within membrane-bound phagosomes. A …

Cigarette Smoking: An Independent Risk Factor For Impotence?, David M. Mannino, R. Monina Klevens, W. Dana Flanders

David M. Mannino

The authors sought to determine whether current cigarette smoking was associated with impotence among middle-aged men. This is a secondary analysis of a cross-sectional survey of 4,462 US Army Vietnam-era veterans aged 31–49 years who took part in the Vietnam Experience Study in 1985–1986. The main outcome measurement was the odds ratio for reported impotence, which was calculated by comparing current smokers with nonsmokers while controlling for multiple confounders. The study sample consisted of 1,162 never smokers, 1,292 former smokers, and 2,008 current smokers. The prevalence of impotence was 2.2% among never smokers, 2.0% among former smokers, and 3.7% among …

Pulmonary Lesions In Fetuses Exposed In Utero To Porcine Reproductive And Respiratory Syndrome Virus, Kelly M. Lager, Mark R. Ackermann

Mark R. Ackermann

The salient features of a porcine reproductive and respiratory syndrome (PRRS) epizootic include respiratory disease followed by reproductive failure. The respiratory component is usually recognized first in finishing pigs or in the breeding herd as a mild flu-like disease with a high morbidity for all ages and high mortality in younger pigs. In breeding herds, acute maternal reproductive failure often follows the onset of respiratory disease. PRRS is characterized by a sudden increase in early farrowings, late-term abortions, stillborn and mummified fetuses, weak neonates with high mortality, late returns to estrus, and repeat breeders. No consistent histopathology has been obseived …

Effects Of Rehabilitation For The Improvement Of Fitness Levels For The Acute Spinal Cord Injured Population, Sandee Melton, Sarah Morrison

Sarah Morrison, PT, MBA, MHA

Reducing Respiratory Artifacts In Chest Mr Images Through Hybrid Space Motion Tracking And Postprocessing, John N. Campbell, Wesley E. Snyder, Peter Santago Ii, Sarah A. Rajala, Craig A. Hamilton

Sarah A. Rajala

A new postprocessing method of correcting for respiratory motion induced artifacts in MRI is presented. The motion of the chest during respiration is modeled as a combination of translation and dilation. Displacements of the chest wall are tracked via a thin, MR-sensitive plate placed on the patient's chest during a scan. Scanning with phase encoding left/right (L/R) and frequency encoding anterior/posterior (A/P) causes the motion artifacts to be repeated in the L/R direction, thus not overlapping on the plate. By performing the inverse A/P Fourier transform, the resulting hybrid space data has A/P spatial data and L/R spatial frequency data, …

Distribution Of Anti-Cd68 (Ebm11) Immunoreactivity In Formalin-Fixed, Paraffin-Embedded Bovine Tissues, Mark R. Ackermann, B. M. Debey, T. J. Stabel, J. H. Gold, K. B. Register, J. T. Meehan

Mark R. Ackermann

A commercially acquired anti-human macrophage antibody (anti-CD68; EBM11) was used in an immunocytochemical technique to detect macrophages in formalin-fixed, paraffin-embedded tissues from cattle, pigs, humans, rats, turkeys, dogs, and cats. In healthy cattle, the antibody labeled alveolar macrophages, pulmonary intravascular cells (presumably intravascular macrophages), and macrophage-like cells in other tissues. In bovine lungs infected with Pasteurella haemolytica, EBM11 antibody labeled 95% of alveolar macrophages and macrophages within alveolar septa but only 0–2% of streaming or “oat” leukocytes. Alveolar macrophages were also stained by EBM11 in pigs but not in rats, turkeys, dogs, and cats. The antibody also stained macrophage aggregates …

Phenotypic Expression Of Recombinant Plasmids Pkt107 And Phk11 In An Avirulent Avian Escherichia Coli, Richard E. Wooley, Lisa K. Nolan, John Brown, Penelope S. Gibbs, Denise I. Bounous

Lisa K. Nolan

An avirulent wild-type avian Escherichia coli strain (Av) was electrotransformed with plasmids coding for complement resistance (pKT107) and Colicin V (ColV) production (pHK11) in order to study the effects of complement resistance and ColV production on virulence. Transformants were also compared with the wild type for embryo lethality, uptake by macrophages, motility, growth rate, plasmid content, and hemolysis. Growth rates and complement resistance patterns of strain Av and transformant Av+pHK1 1 were similar, but Av+pHK11 caused a significantly greater number of deaths in embryos and acquired motility. Transformant Av+pKT107 had a lower rate of phagocytosis, a slower growth rate, and …

Characterization Of An Avirulent Mutant Of A Virulent Avian Escherichia Coli Isolate, Lisa K. Nolan, Richard E. Wooley, Catherine W. Giddings, John Brown

Lisa K. Nolan

A virulent, complement-resistant avian Escherichia co/lisolate and its avirulent, complement-sensitive, transposon-insertion mutant were compared for the purpose of revealing structures associated with complement resistance. Both had a smooth lipopolysaccharide layer, contained traT, and lacked a capsule, but the mutant possessed a 16.2-kilodalton outer-membrane protein (OMP) not present in the wild-type. This protein may be the product of a coding region interrupted by transposon insertion. Such results suggest that an OMP greater than 16.2 kilodaltons in size may be responsible for the complement resistance and virulence of this wild-type E. coli.

Infected Cell Types In Ovine Lung Following Exposure To Bovine Respiratory Syncytial Virus, J. T. Meehan, R. C. Cutlip, H. D. Lehmkuhl, J. P. Kluge, Mark R. Ackermann

Mark R. Ackermann

Sixteen adult sheep (ten females, six males obtained from a closed flock at National Animal Disease Center, Ames, IA) were experimentally infected with bovine respiratory syncytial virus strain 375 (BRSV), and lung tissues were stained for viral antigen. Two infected sheep were euthanatized at each of the following post-inoculation times: 12, 24, 36, 48, 72, 96, 144, and 192 hours. Lung, nasal turbinates, trachea, right cranial bronchial and mediastinal lymph nodes, liver, and spleen were collected for histologic evaluation. An indirect immunoperoxidase technique was performed on routine paraffin-embedded sections of lung tissue, trachea, turbinates, and bronchial and mediastinal lymph nodes …

Effect Of Normal Intestinal Flora Of Chickens On Colonization By Virulent Colicin V-Producing, Avirulent, And Mutant Colicin V-Producing Avian Escherichia Coli, Richard E. Wooley, John Brown, Penelope S. Gibbs, Lisa K. Nolan, Kathy R. Turner

Lisa K. Nolan

Colonization of the intestinal tracts of newly hatched chicks with Escherichia coli was attempted by swabbing test organisms onto the air-shell of 19-day-old embryos. Test organisms consisted of two virulent E. coli isolates, one avirulent isolate, and one laboratory-derived mutant of the avirulent isolate carrying a recombinant plasmid coding for Colicin V production. Chicks were cultured weekly for 3 weeks for total E. coliand for the test organisms using selective media. Control chicks were sampled on weeks 1 and 5, and the normal E. coli intestinal microflora were examined for the production of colicins. The two virulent E. coli isolates …

The Cowboy And Camel Are Strange (Poem), Richard W. Pollay

Richard W. Pollay

No abstract provided.

Exposure Of Youth To U.S. Cigarette Broadcast Advertising In The 1960s, Richard W. Pollay

Richard W. Pollay

No abstract provided.

Promises, Promises: Self-Regulation Of Us Cigarette Broadcast Advertising In The 1960s, Richard W. Pollay

Richard W. Pollay

No abstract provided.

Bullous Pemphigoid--Management Of A Patient With Oral And Skin Lesions, Ali T. B., Rosnah Binti Zain, Adam B. A.

Prof. Dr. Rosnah Binti Zain

A case of bullous pemphigoid with extra-oral and intraoral lesions in a 60-year-old female is reported. Diagnosis is based on histopathology and direct immunofluorescence, and the treatment regime described included oral prescription of prednisolone and topical application of a mixture of fluocinonide ointment and triamcinolone acetonide in Orabase on the oral lesions.

Rural Assistive Technology Hypermedia Decision Support System, Steven A. Freeman, Don D. Jones, William E. Field

Steven A. Freeman

A prototype hypermedia decision support system for the selection and documentation of rural assistive technology (BNG DATA) was developed to aid professionals working with farmers, ranchers, and agricultural workers with physical disabilities. The hypermedia system (constructed using HyperCard, an environment that combines hypertext and database features) consists of a hypermedia database of rural assistive technology examples and an accompanying decision support system that helps users identify solution alternatives to meet the needs of their clientele. End user acceptance of BNG DATA was determined using an evaluation questionnaire. The end users evaluating the prototype considered BNG DATA to be easy to …

Relation Between Phonologic Difficulty And The Occurrence Of Disfluencies In The Early Stage Of Stuttering, Rebecca Throneburg, Ehud Yairi, Elaine P. Paden

Rebecca Throneburg

People who stutter, especially children, have often been reported to exhibit a wide range of concomitant communication problems including articulation and phonologic deficiencies. This study investigated the relation between the phonologic difficulty of words and the point at which stuttering-like disfluencies occurred in the speech of preschool children identified as having a stuttering problem (n = 24). The children were divided into subgroups according to stuttering severity and phonologic ability. A spontaneous speech sample of approximately 1,000 words was tape-recorded from each child, and perceived disfluencies were identified. The phonologic difficulty of each word on which there was a stuttering-like …

Patterns Of Interaction In The Lexical Development In Two Languages Of Bilingual Infants, Barbara Pearson, Sylvia Fernandez

Barbara Zurer Pearson

We investigated the extent to which bilingual children follow the same patterns and timetable of lexical development as monolinguals. For a group of 20 simultaneous bilingual (English-Spanish) infants, ages 10 to 30 months, we looked at the patterns of growth in one language in relation to growth in the other and also with respect to growth in both languages combined. The MacArthur Communicative Development Inventories (CDI), standardized parent report forms in Spanish and English, provided measures of lexical growth in two languages at varying intervals within the age range. We plotted the two single-language measures, as well as Total and …

Leader-Member Exchange (Lmx) Scale, Terri A. Scandura

Terri A. Scandura

Scale used for LMX research. Supplementary material to: Scandura, T.A. & Schrieshiem, C.A. (1994). Leader-member exchange and supervisor career mentoring as complementary constructs in leadership research, Academy of Management Journal, 37, 1588-1602.

A Cost Benefit Analysis Of Placing Rotary Supported Aero-Medical Teams In Strategic Rural Areas, David N. Reid

David N Reid

Completed a part of an undergraduate thesis, this work studied the economic value of placing aeromedical retrieval and rescue helicopters in strategic rural locations.

The Activator Methods Story: Development Of A New Concept In Chiropractic., Dennis M. Richards

Dennis M Richards

This paper documents the development of Activator Methods Chiropractic Technique and the lives of its founders, Drs Warren Lee and Arlan Fuhr.

Fitness For The Spinal Cord Population: Establishing A Program In Your Facility, Sarah Morrison

Sarah Morrison, PT, MBA, MHA

No abstract provided.

Early Identification And Referral Of Children With Language Disorders. The Joint Role Of The General Practitioner And Speech-Language Therapist, Frances C. Slabbert, Brenda Louw

Brenda Louw

Thank The Editors For The Buy-Ological Urge! American Magazines, Advertising And The Promotion Of The Consumer Culture, 1920-1980, Richard W. Pollay

Richard W. Pollay

No abstract provided.