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Full-Text Articles in Medicine and Health Sciences
Comparison Of Different Pinning Techniques For 3d-Printed Radius With Salter-Harris Type I Fracture, Tea-Hyung Kim, Hwi-Yool Kim
Comparison Of Different Pinning Techniques For 3d-Printed Radius With Salter-Harris Type I Fracture, Tea-Hyung Kim, Hwi-Yool Kim
The Thai Journal of Veterinary Medicine
The purpose of the present study was to compare different pinning techniques for distal radius with Salter-Harris type I fracture. Computed Tomography (CT) images of a distal antebrachium of the 6.5 kg, growing, 6-month-old intact male mongrel dog were 3D-printed with Polycarbonate (PC) material. Twenty-one synthetic bones were classified into three groups. Cross pinning, Parallel pinning, and HK2 pinning techniques were applied to each group. A compressive shear test was applied to each group, and the results at 1 mm, 2 mm, and 3 mm displacement and the load at failure were analyzed through statistical software. It was found that …
Computed Tomographic Findings Of Primary Renal Tumors In Dogs And Cats, Daji Noh, Jaejin Shim, Sooyoung Choi, Hojung Choi, Youngwon Lee, Kija Lee
Computed Tomographic Findings Of Primary Renal Tumors In Dogs And Cats, Daji Noh, Jaejin Shim, Sooyoung Choi, Hojung Choi, Youngwon Lee, Kija Lee
The Thai Journal of Veterinary Medicine
Primary renal tumor is uncommon in dogs and cats, with few previous computed tomography (CT) reports. Previous studies have reported that contrast-enhancing CT is helpful in differentiating the type of renal tumors. This study aimed to describe CT findings of renal tumors in dogs and cats and identify contrast-enhancing pattern according to 3 post-contrast phases. In this retrospective study, the following CT findings were recorded for each patient: (1) renal tumor involvement, (2) enhancement pattern, (3) vascular invasion, (4) presence of lymphadenopathy and organ metastasis, (5) presence of mineralization, and (6) attenuation values of renal tumors on pre- and post-contrast …
Enterococcus Faecium Septicemia In A Dog: Clinical Features And Molecular Screening Of Virulence Genes Of Enterococcus, Trasida Ployngam, Numfa Fungbun
Enterococcus Faecium Septicemia In A Dog: Clinical Features And Molecular Screening Of Virulence Genes Of Enterococcus, Trasida Ployngam, Numfa Fungbun
The Thai Journal of Veterinary Medicine
A one-year-old Golden Retriever male dog manifested multi-systemic inflammation with a marked increment of plasma D-dimer. Enterococcus spp. was identified from hemoculture and it showed multidrug resistance (MDR), including amikacin, cephazolin, ceftriaxone, clindamycin, imipenem, penicillin G and sulfa-trimethoprim. Pure Enterococcus colonies were identified for species-specific DNA by PCR amplification and sequencing. It was 100% identical with the Enterococcus faecium strains in the National Center for Biotechnology Information (NCBI) database. Molecular screening of virulence genes of the isolated E. faecium strain exhibited four of nine genes including gelatinase (gelE), cell wall adhesion (efaAfm), second collagen adhesin (scm) and adhesin of collagen …
Suspected Malignant Mesothelial Cells In Bronchoalveolar Lavage Fluid Of A Dog With Respiratory Distress, Junseok Kim, Jimin Oh, Taesik Yun, Yoonhoi Koo, Dohee Lee, Yeon Chae, Byeong-Teck Kang, Mhan-Pyo Yang, Hakhyun Kim
Suspected Malignant Mesothelial Cells In Bronchoalveolar Lavage Fluid Of A Dog With Respiratory Distress, Junseok Kim, Jimin Oh, Taesik Yun, Yoonhoi Koo, Dohee Lee, Yeon Chae, Byeong-Teck Kang, Mhan-Pyo Yang, Hakhyun Kim
The Thai Journal of Veterinary Medicine
An 11-year-old neutered female Labrador Retriever presented with a 1-week history of respiratory distress, lethargy and anorexia. On physical examination, the dog exhibited dyspnea with a rapid and shallow breathing pattern. Thoracic radiography showed diffuse nodular or miliary interstitial patterns, which raised suspicion of bacterial, fungal and parasitic infections and metastatic tumors. Bronchoalveolar lavage (BAL) was performed and the culture yielded no growth of infectious agents, including bacteria and fungi. A presumptive diagnosis of pulmonary mesothelioma was made based on malignant mesothelial cells on BAL fluid cytology. Therefore, this is a report identifying suspected pulmonary mesothelioma using BAL fluid in …
Inconsistent And Multiple Bacterial Species From Different Sample Types Of Dogs With Urolithiasis And Bacterial Cystitis, Nantapatt Pridsadangbud, Chantamanee Chantarasiri, Nattanit Visitkitjakarn, Phitsinee Tantivimonkajor, Jitrapa Yindee, Nicole Sirisopit Mehl
Inconsistent And Multiple Bacterial Species From Different Sample Types Of Dogs With Urolithiasis And Bacterial Cystitis, Nantapatt Pridsadangbud, Chantamanee Chantarasiri, Nattanit Visitkitjakarn, Phitsinee Tantivimonkajor, Jitrapa Yindee, Nicole Sirisopit Mehl
The Thai Journal of Veterinary Medicine
Urinary tract infection is the most common complication found in dogs with urolithiasis. Bacterial cystitis can play an important role in dogs with urolithiasis; it can either be the cause of cystic formation or be a complication following urolithiasis. Moreover, urolithiasis cases that come with bacterial cystitis can lead to a condition of complicated cystitis which will require more complicated treatment and management. Proper bacterial identification should be performed in order to follow an appropriate treatment. Specific treatments require bacterial identification in order to select the most appropriate antibiotics. There are three main possible sites for bacterial sample collection in …
Assessment Of Abdominal Fat Distribution By Computed Tomography In Obese Dogs, Yunseo Jang, Hyemin Na, Sooyoung Choi, Hojung Choi, Youngwon Lee, Kija Lee
Assessment Of Abdominal Fat Distribution By Computed Tomography In Obese Dogs, Yunseo Jang, Hyemin Na, Sooyoung Choi, Hojung Choi, Youngwon Lee, Kija Lee
The Thai Journal of Veterinary Medicine
Obesity is a risk factor for canine health but there has been no study on the tendency of body fat accumulation naturally occurring in obese dogs. This retrospective observational study aims to evaluate the tendency to accumulate visceral and subcutaneous fat using computed tomography (CT) in naturally occurring obese dogs. The study included 15 dogs with a body condition score of 8 or 9 that underwent abdominal CT scans. Total fat area (TA), visceral fat area (VA), subcutaneous fat area (SA) and body area (BA) were measured by non-contrast transverse image at the third lumbar vertebra (L3) and the sixth …
Indications For Canine Fecal Microbiota Transplantation, Kerong Li, Xiaoxiao Zhou, Zhijun Zhong, Haifeng Liu, Min Li, Guangneng Peng, Ziyao Zhou
Indications For Canine Fecal Microbiota Transplantation, Kerong Li, Xiaoxiao Zhou, Zhijun Zhong, Haifeng Liu, Min Li, Guangneng Peng, Ziyao Zhou
The Thai Journal of Veterinary Medicine
Fecal microbiota transplantation (FMT) is a novel method to treat intestinal and extraintestinal diseases by transplanting the microbiota in the feces of healthy animals into the gastrointestinal tracts of patients and reconstructing intestinal flora in the patients' intestine. In this article, we reviewed the recent studies of canine FMT focusing on its indications. FMT was proved effective to treat canine Clostridioides difficile infection (CDI), inflammatory bowel disease (IBD), canine parvovirus (CPV) diarrhea and acute diarrhea; upregulate the intestinal microbiota in dogs with acute hemorrhagic diarrhea syndrome (AHDS); and may have benefit for preventing canine post-weaning diarrhea. However, the specific FMT …
A Dorsal Sling Technique In A Dermoid Sinus Dog With Nuchal Ligament Excision, Nithida Boonwittaya, Pakthorn Lewchalermwong, Kanokwan Keadwut
A Dorsal Sling Technique In A Dermoid Sinus Dog With Nuchal Ligament Excision, Nithida Boonwittaya, Pakthorn Lewchalermwong, Kanokwan Keadwut
The Thai Journal of Veterinary Medicine
A dorsal sling technique for correction of nuchal ligament excision in a dermoid sinus canine case is presented in this report. At surgery, there were multiple tubes of the dermoid sinus tracts of different types. The nuchal sinus was treated using a segmental resection at the area of involvement and a dorsal sling technique was performed. The dog recovered uneventfully and regained normal daily activities within a short time after surgery. The technique was accomplished as evaluated by non-pain normal motion of the cervical region and no complications were found during the follow-up period. This is the first report on …
Diagnostic Utility Of Basal Serum T4 And Tsh Concentrations In Dogs With Hypothyroidism Confirmed By Tsh Stimulation Test, Minkeun Shin, Dohee Lee, Taesik Yun, Yoonhoi Koo, Byeong-Teck Kang, Mhan-Pyo Yang, Hakhyun Kim
Diagnostic Utility Of Basal Serum T4 And Tsh Concentrations In Dogs With Hypothyroidism Confirmed By Tsh Stimulation Test, Minkeun Shin, Dohee Lee, Taesik Yun, Yoonhoi Koo, Byeong-Teck Kang, Mhan-Pyo Yang, Hakhyun Kim
The Thai Journal of Veterinary Medicine
The objective of this study was to evaluate the diagnostic performance of a basal serum thyroxine (T4) and thyrotropin (TSH) combination test for the diagnosis of canine hypothyroidism in clinical practice. It was hypothesized that the usefulness of basal T4 and TSH combination test in dogs with suspected non-thyroidal illness syndrome would be different with that in previous studies. The data of this study were searched for dogs who underwent both TSH stimulation test and basal serum T4 and TSH combination test. Forty-four cases were classified into either of three groups (hypothyroid, intermediate, or euthyroid) according to the results of …
Butterfly Pea Flower Extract As An Alternative Dye In Cytological Canine Mast Cell Tumor Staining, Araya Suebkhampet, Pongsiwa Sotthibandhu, Titaree Laoharatchatathanin
Butterfly Pea Flower Extract As An Alternative Dye In Cytological Canine Mast Cell Tumor Staining, Araya Suebkhampet, Pongsiwa Sotthibandhu, Titaree Laoharatchatathanin
The Thai Journal of Veterinary Medicine
Mast cell tumors (MCTs) are the most common skin tumors in dogs. Laboratory diagnosis of MCTs has been established by many techniques.The dyes generally used for tissue staining, including MCTs, are synthetic dyes, however, the global interest in using eco-friendly natural dye has become a significant matter. We focused on the butterfly pea (Clitoria ternatea L.) which is locally available. Limited studies are known about their efficacy as a tissue stain. The aim of this study was to investigate the staining ability of butterfly pea flower extract in cytological canine MCTs. The dried petals were ground into powder and dissolved …
Hyperdynamic Circulatory State In A Dog With Leptospirosis: A Case Report, Numfa Fungbun, Naruepon Kampa, Supranee Jitpean, Nitaya Boonbal
Hyperdynamic Circulatory State In A Dog With Leptospirosis: A Case Report, Numfa Fungbun, Naruepon Kampa, Supranee Jitpean, Nitaya Boonbal
The Thai Journal of Veterinary Medicine
A hyperdynamic circulatory state is a common consequence of portal vein hypertension. Interestingly, this case report demonstrates acute canine leptospirosis manifesting with abdominal effusion, which it is focused ona hyperdynamic circulatory state, caused by portal hypertension. Severe acute kidney injury caused by leptospirosis in this case was hypothesized to be aggravated by renal hypoperfusion. This condition was noticed to occur in a similar way to hepatorenal syndrome (HRS) in humans. Briefly, a dog with leptospirosis manifesting with abdominal effusion which was diagnosed to be the consequence of hyperdynamic circulatory state caused by hepatopathy and severe acute kidney injury. Peritoneal dialysis …
An Appraisal Of Canine Transmissible Venereal Tumour With Emphasis On Molecular Biology And Pathology, Iniobong Chukwuebuka Ikenna Ugochukwu, Onyinyechukwu Ada Agina, Jacinta Ngozi Omeke, Chioma Inyang Aneke, Lawan Fatima Adamu, Olusola Lawrence Ajayi, Najume Dogowar Giginya Ibrahim, Celestine Ibe Njoku, Anthony Kojo Beku Sackey, John Ikechukwu Ihedioha
An Appraisal Of Canine Transmissible Venereal Tumour With Emphasis On Molecular Biology And Pathology, Iniobong Chukwuebuka Ikenna Ugochukwu, Onyinyechukwu Ada Agina, Jacinta Ngozi Omeke, Chioma Inyang Aneke, Lawan Fatima Adamu, Olusola Lawrence Ajayi, Najume Dogowar Giginya Ibrahim, Celestine Ibe Njoku, Anthony Kojo Beku Sackey, John Ikechukwu Ihedioha
The Thai Journal of Veterinary Medicine
Canine transmissible venereal tumour (CTVT) is a contagious tumour of dogs, transmitted via coitus or coital behaviour which in some cases spreads by metastasis but primarily, CTVT appears as reddish soft nodules or papilla, protruding from the surface of the penis, prepuce, vagina and vulva but sometimes appears in locations outside the genitals. There was early evidence of CTVT more than 10,000 years ago and this disease has been reported in at least 90 countries across the continents of the world, especially in third world countries, where there are high numbers of stray dogs. CTVT natural infection occurs only in …
Selection Of The Appropriate Reference Genes For Relative Quantitative Reverse Transcription Polymerase Chain Reaction (Qrt-Pcr) In Canine Pulmonary Arteries, Nattawan Tangmahakul, Somporn Techangamsuwan, Sirilak Disatian Surachetpong
Selection Of The Appropriate Reference Genes For Relative Quantitative Reverse Transcription Polymerase Chain Reaction (Qrt-Pcr) In Canine Pulmonary Arteries, Nattawan Tangmahakul, Somporn Techangamsuwan, Sirilak Disatian Surachetpong
The Thai Journal of Veterinary Medicine
A normalisationof targeted genes using the appropriate reference genes is necessary for reliable quantitative reverse transcription polymerase chain reaction (qRT-PCR) experiments. This study aimed to investigate the appropriate reference genes and the numbers of the genes required for qRT-PCR in canine pulmonary arteries. Pulmonary arteries were collected from twenty-three dogs at necropsy. Candidate reference genesincluding ribosomal protein L32 (RPL32), ribosomal protein L13a (RPL13A), ribosomal protein S18 (RPS18), TATA box binding protein (TBP), beta-2-microglobulin (B2M), ribosomal protein S5 (RPS5), ribosomal protein S19 (RPS19) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from pulmonary arteries were examined by qRT-PCR and analysed using four major algorithms, including …
Prevalence Of Canine Parvovirus And Rotavirus With Diarrhea In Western Taiwan, Chiu-Huang Ting, Chia-Ying Lin, Hung-Yi Wu, Hung-Yi Wu, Yueh-Fang Lee, Ching-Dong Chang, Shyh-Shyan Liu
Prevalence Of Canine Parvovirus And Rotavirus With Diarrhea In Western Taiwan, Chiu-Huang Ting, Chia-Ying Lin, Hung-Yi Wu, Hung-Yi Wu, Yueh-Fang Lee, Ching-Dong Chang, Shyh-Shyan Liu
The Thai Journal of Veterinary Medicine
In this study, we investigated the prevalence of Diarrhea pathogens in pet dogs referred to as Canine Parvovirus (CPV) and Canine Rotavirus (CRV)in western Taiwan using conventional polymerase chain reaction (PCR).Fecalsampleswere collected from240 dogs which have the symptomsofdiarrhea examined bythe veterinaryhospitals from March 2015 to March 2017.The PCR sensitivityof total DNAs extracted from 0.1g fecal samples rangedfrom 10ng to100 ng. The prevalence of CPV and CRV infections were23.3% (56/240) and 9.2% (22/240)respectively.The related analysis between prevalence rates and the epidemiological data of pet dogs were correlated with the age, season,area, vaccinationandbreed. The results showed that both the diseases have the …
Review Of The Cardiovascular Toxicity Of Amitriptyline Treatment For Canine Neuropathic Pain, Saikaew Sutayatram, Kumpanart Soontornvipart, Piyasiri Glangosol
Review Of The Cardiovascular Toxicity Of Amitriptyline Treatment For Canine Neuropathic Pain, Saikaew Sutayatram, Kumpanart Soontornvipart, Piyasiri Glangosol
The Thai Journal of Veterinary Medicine
Nowadays, the lifespan of pets has been increased by improvements in veterinary practice but age-related disorders are also increasing. Several nervous system attenuations are well-known to be the result of aging and/or pathological lesions. In many cases, neuropathic pain (NP) can develop and cause a significant impact on the clinical outcome and quality of life. With the difficulty in identification of the specific cause of NP, most NP management is planned according to clinical manifestations and recommended trial therapy. The drug of choice for trial therapy seems to be amitriptyline, a tricyclic antidepressant with multiple effects on neurotransmitter receptors and …
Immunohistochemical Staining Of Peroxisome Proliferatoractivated Receptor Alpha And Gamma In Normal, Benign, And Malignant Canine Mammary Tissues, Wuthichai Klomkleaw, Promporn Raksaseri
Immunohistochemical Staining Of Peroxisome Proliferatoractivated Receptor Alpha And Gamma In Normal, Benign, And Malignant Canine Mammary Tissues, Wuthichai Klomkleaw, Promporn Raksaseri
The Thai Journal of Veterinary Medicine
The peroxisome proliferator-activated receptors (PPARs) belong to nuclear receptor superfamily acting as transcription factors related to lipid and glucose metabolism. There are 3 subtypes including PPARα, PPAR, and PPAR. PPARα and PPAR activations can inhibit tumor cell proliferation and differentiation in many cell types. However, the expressions of these receptors in canine mammary tissue are still unknown. This study was performed to investigate the expressions of PPARα and PPAR in normal, benign, and malignant canine mammary tissues. Twenty four bitches at the age of 5-15 years old, which undergone unilateral mastectomy, were used in the study. Mammary tissues were sectioned …
Detection Of Transforming Growth Factor Beta 1 In Normal Dogs And Dogs With Degenerative Mitral Valve Disease, Wiyada Winyuchonharoen, Somporn Techangamsuwan, Sirilak Disatian Surachetpong
Detection Of Transforming Growth Factor Beta 1 In Normal Dogs And Dogs With Degenerative Mitral Valve Disease, Wiyada Winyuchonharoen, Somporn Techangamsuwan, Sirilak Disatian Surachetpong
The Thai Journal of Veterinary Medicine
Degenerative mitral valve disease (DMVD) is a common finding of acquired heart diseases in small and medium-sized adult dogs. Transforming growth factor beta 1 (TGF-β1) was found to increase in valves of DMVD dogs. However, plasma TGF-β1 concentrations in dogs with DMVD have not been evaluated before. The objective of the present study was to compare the plasma concentration of TGF-β1 between normal dogs and dogs with DMVD by using an ELISA technique. This measurement might help to distinguish dogs with DMVD from healthy dogs. The Mann-Whitney U test was used to compare plasma TGF-β1 concentrations between the two groups. …
Prevalence Of Bacteremia In Dogs Admitted To An Intensive Care Unit With Intravenous Catheterization, Pattrarat Chanchaithong, Sukullaya Ritthikulprasert
Prevalence Of Bacteremia In Dogs Admitted To An Intensive Care Unit With Intravenous Catheterization, Pattrarat Chanchaithong, Sukullaya Ritthikulprasert
The Thai Journal of Veterinary Medicine
Bacteremia is a common complication in hospitalized patients, and intravenous catheterization is a routine intervention in dogs in intensive care units (ICU). This study aimed to investigate bacteria associated with bacteremia in an ICU of a veterinary teaching hospital during 2013-2014. Fifty dogs admitted to the ICU were included in this study. Twenty-four dogs (48.00%) presented bacteremia by blood culture. Bacterial species were identified, including Klebsiella pneumoniae (8/24), Pseudomonas aeruginosa (7/24), Staphylococcus pseudintermedius (3/24), Escherichia coli (2/24), Bacillus sp. (1/24), Acinetobacter sp. (2/24) and Burkholderia cepacia (1/24). Only five dogs had positive catheter colonization by identical bacterial species with those …
Management Of Multiple Intracranial Granular Cell Tumors In A Golden Retiever, Pichanun Linharattanaruksa, Pakthorn Lewchalermwong, Muhammad Khalid, Kannika Siripattarapravat
Management Of Multiple Intracranial Granular Cell Tumors In A Golden Retiever, Pichanun Linharattanaruksa, Pakthorn Lewchalermwong, Muhammad Khalid, Kannika Siripattarapravat
The Thai Journal of Veterinary Medicine
A 9-year-old, neutered female Golden Retriever was presented with generalized seizures, abnormal mentation and ataxia. Magnetic resonance imaging revealed five separated masses at the left dorsal meninges, caudodorsal cerebellum, right cerebral cortex, olfactory lobe and right occipital lobe. Right rostrotentorial craniectomy was performed to debulk the tumor at the right cerebral cortex. On the basis of histopathological evaluation, the mass was diagnosed as GCTs. Immunohistochemistry of the mass demonstrated immunopositivity for vimentin and immunonegativity for GFAP, NSE, Desmin, S-100, CK14, CK8/18, CK19, and Ki67. Postoperative neurological deficits were resolved, but six months later neurological signs recurred with higher frequency of …
Electrocardiographic Changes In Dogs With Degenerative Mitral Valve Disease Treated With Pimobendan: A Retrospective Study Of 29 Cases, Sirilak Disatian Surachetpong, Nisarat Boonlue, Pasorn Pupa, Waratsarin Boonsathitanan, Sumanee Rakthaidee, Tanawan Mangklabruks, Siriwan Sakarin
Electrocardiographic Changes In Dogs With Degenerative Mitral Valve Disease Treated With Pimobendan: A Retrospective Study Of 29 Cases, Sirilak Disatian Surachetpong, Nisarat Boonlue, Pasorn Pupa, Waratsarin Boonsathitanan, Sumanee Rakthaidee, Tanawan Mangklabruks, Siriwan Sakarin
The Thai Journal of Veterinary Medicine
The aim of this retrospective study was to evaluate changes in electrocardiograms (ECG) and cardiac arrhythmias in 29 dogs with degenerative mitral valve disease (DMVD) treated with pimobendan chronically. All dogs had normal ECG before pimobendan administration. The dogs were classified according to ECG after pimobendan administration into 2 groups: normal ECG (n=19) and abnormal ECG (n=10). Age, sex, breed, serum alkaline phosphatase, concurrent digoxin administration, total daily dosage of pimobendan and digoxin, duration of pimobendan administration, and ECG parameters including heart rate, P wave, PR interval, QRS complex, and QT interval were analyzed. Cardiac arrhythmias were increasingly found in …
Selection Of Reference Genes For Real-Time Polymerase Chain Reaction In Canine Oral Tumor And Cancer, Sirinun Pisamai, Anudep Rungsipipat, Chanin Kalpravidh, Gunnaporn Suriyaphol
Selection Of Reference Genes For Real-Time Polymerase Chain Reaction In Canine Oral Tumor And Cancer, Sirinun Pisamai, Anudep Rungsipipat, Chanin Kalpravidh, Gunnaporn Suriyaphol
The Thai Journal of Veterinary Medicine
Tumors in oral cavity either benign or malignant are often found in dogs. Despite the increasing number of studies of gene expression associated with canine oral cavity diseases, reference gene selection for normalizing target genes has not been performed. The objective of the present study was to identify potential reference genes in canine oral tumors, including epulis, ameloblastoma, oral melanoma, oral squamous cell carcinoma, etc. Suitability of 6 candidate reference genes, beta-actin (ACTB), beta-2-microglobulin (B2M), glyceraldehydes-3-phosphate dehydrogenase (GAPDH), ribosomal subunit L13a (RPL13a), ribosomal protein S5 (RPS5) and ribosomal protein S19 (RPS19), was evaluated with 5 algorithms, geNorm, Normfinder, BestKeeper, the …
Involucrin Expression And Association With Ki-67 In Paraffin Embedded Tissue Of Canine Skin Tumors: A Retrospective Study, Nongnut Assawawongkasem, Gunnaporn Suriyaphol, Sayamon Srisuwattanasagul, Sirin Theerawatanasirikul, Achariya Sailasuta
Involucrin Expression And Association With Ki-67 In Paraffin Embedded Tissue Of Canine Skin Tumors: A Retrospective Study, Nongnut Assawawongkasem, Gunnaporn Suriyaphol, Sayamon Srisuwattanasagul, Sirin Theerawatanasirikul, Achariya Sailasuta
The Thai Journal of Veterinary Medicine
Involucrin (INV) is a cornified envelope precursor protein that is commonly used as an early stage marker of human terminal keratinocyte differentiation. This research investigated the expression of INV in canine skin tumors using 56 formalin-fixed paraffin-embedding (FFPE) biopsied specimens. The expression of INV was investigated by immunohistochemistry (IHC) and RT-PCR. Correlation between INV and Ki-67 was also performed. The malignant tumor groups showed significantly different INV staining scores and Ki-67 positive cells than the normal skin group by IHC (p<0.05). The INV scores of moderately differentiated SCC (MSCC), trichoblastoma (TCB), basal cell carcinoma (BCC) and poorly differentiated SCC (PSCC) significantly decreased, with a statistically significant difference (p<0.05). Interestingly, the INV of poorly differentiated SCC disappeared in some specimens. The INV expression in most tumors was found to be inversely proportionate to Ki-67 positive cells. In addition, only SCC regardless of tumor grade without tumor-graded specimens showed an inversely statistically significant difference between the Ki-67 positive cells and INV expression (p<0.0001). In RT-PCR, mRNA expression of the FFPE specimens showed substantial similarity of INV expression as in IHC. It is suggested that the INV expression should be further studied in terms of clinical prognosis in canine cutaneous SCC.
Factors Affecting Treatment Outcome Of Canine Transmissible Venereal Tumor: A Retrospective Study, Pansawut Sudjaidee, Suppawiwat Ponglowhapan, Padet Tummaruk, Kaywalee Chatdarong
Factors Affecting Treatment Outcome Of Canine Transmissible Venereal Tumor: A Retrospective Study, Pansawut Sudjaidee, Suppawiwat Ponglowhapan, Padet Tummaruk, Kaywalee Chatdarong
The Thai Journal of Veterinary Medicine
Canine transmissible venereal tumor (TVT) is a contagious neoplastic disease commonly seen in tropical and subtropical areas in the countries where the control of stray dog population is poor. This study aimed to summarize the current disease status and evaluate factors affecting treatment outcome to vincristine sulfate, which is the chemotherapeutic drug of choice. Studied factors were divided into three categories; general background (i.e. age, gender, breed), specific descriptions (i.e. tumor diameter, tumor site, season of the year), and treatment details (number of vincristine shots, meteorological parameters). One hundred and fifty-seven medical records were included with 100 achieved complete treatments …
Low-Dose Cyclophosphamide And Piroxicam Inhibit Growth, Migration, And Invasion Of Canine Oral Malignant Melanoma Cell Line, Nan Choisunirachon, Kota Yoshida, Kohei Saeki, Yuiko Tanaka, Manabu Mochizuki, Ryohei Nishimura, Nobuo Sasaki, Takayuki Nakagawa
Low-Dose Cyclophosphamide And Piroxicam Inhibit Growth, Migration, And Invasion Of Canine Oral Malignant Melanoma Cell Line, Nan Choisunirachon, Kota Yoshida, Kohei Saeki, Yuiko Tanaka, Manabu Mochizuki, Ryohei Nishimura, Nobuo Sasaki, Takayuki Nakagawa
The Thai Journal of Veterinary Medicine
The effect of low-dose chemotherapy on tumor motility of canine oral malignant melanoma has been investigated on an in vitro model using low-dose cyclophosphamide and piroxicam. Cyclophosphamide, at a concentration of 0.1 mg/mL in culture medium, significantly inhibited tumor cell migration/invasion through trans-well assays and suppressed both ERK and NF-κB signaling, the latter of which was observed through IκB-α and P-IκBα expression. Furthermore, in combination with a COX-2 inhibitor, i.e. piroxicam (0.38 mg/mL in culture medium), cyclophosphamide revealed a potential anti-migration effect over the single piroxicam treatment on a wound assay. This result suggested that low-dose cyclophosphamide might have influence …
Effect Of Pcso-524 On Oa Biomarkers And Weight-Bearing Properties In Canine Shoulder And Coxofemeral Osteoarthritis, Kumpanart Soontornvipart, Natwadee Mongkhon, Korakot Nganvongpanit, Prachya Kongtawelert
Effect Of Pcso-524 On Oa Biomarkers And Weight-Bearing Properties In Canine Shoulder And Coxofemeral Osteoarthritis, Kumpanart Soontornvipart, Natwadee Mongkhon, Korakot Nganvongpanit, Prachya Kongtawelert
The Thai Journal of Veterinary Medicine
This study was designed to compare the therapeutic benefits of a compound of omega-3 fatty acids from the New Zealand green-lipped mussel (Perna canaliculus) (PCSO-524) and omega-3 fatty acids in fish oil on clinical outcomes and osteoarthritis biomarkers (chondroitin sulfate WF6 epitope) in 66 dogs that had osteoarthritis (OA); 39 dogs with OA hip joints, 15 dogs with OA shoulder joints and 12 dogs with OA shoulder and hip joints. The animals were presented at the Small Animal Hospital, Faculty of Veterinary Science, Chulalongkorn University. The dogs were allocated into two groups randomly. One group received PCSO-524 (n = 33) …
Proliferative Index And Microvessel Density As Potential Prognostic Markers Of Canine Oral Malignant Melanomas, Nan Choisunirachon, Yuiko Tanaka, Kohei Saeki, Nobuo Sasaki, Ryohei Nishimura, Takayuki Nakagawa
Proliferative Index And Microvessel Density As Potential Prognostic Markers Of Canine Oral Malignant Melanomas, Nan Choisunirachon, Yuiko Tanaka, Kohei Saeki, Nobuo Sasaki, Ryohei Nishimura, Takayuki Nakagawa
The Thai Journal of Veterinary Medicine
Several reports have indicated the prognostic significance of the proliferative index (PI) and microvessel density (MVD) in canine oral malignant melanomas (MM). However, a correlation between the PI and MVD of oral MM in dogs has never been elucidated. Here, we evaluated the expression of Ki-67 and CD31 using double immunofluorescence staining to investigate the correlation of PI with MVD in 37 spontaneous canine oral MMs. No correlation was found between the MVD and PI values or between those and any clinicopathologic findings. However, a correlation was found between the PI value and both clinical stage (p = 0.0453) and …
Retrospective Study Of Computed Tomographic Characterization Of Canine Oral Malignant Melanoma In 24 Dogs, Nan Choisunirachon, Lee-Shuan Lin, Yuiko Tanaka, Kohei Saeki, Reina Fujiwara, Ryohei Nishimura, Takayuki Nakagawa
Retrospective Study Of Computed Tomographic Characterization Of Canine Oral Malignant Melanoma In 24 Dogs, Nan Choisunirachon, Lee-Shuan Lin, Yuiko Tanaka, Kohei Saeki, Reina Fujiwara, Ryohei Nishimura, Takayuki Nakagawa
The Thai Journal of Veterinary Medicine
Clinical and computed tomographic (CT) information from 24 histopathological diagnosed canine oral malignant melanomas (MMs) was retrospectively investigated. The CT appearance on canine oral MM from each patient was evaluated, including both bone and soft tissue windows and pre- and post-contrast enhancement. Among the 24 dogs, canine oral MM was highly presented in small breeds, especially in miniature dachshunds older than 10 years. The gingiva was the most commonly affected site, followed by palatine, mucosal, and lingual areas. The location of the primary tumor in the oral arcade was statistically significant associated with bone invasion such as osteolysis. Canine oral …
Cloning Efficiency Of Canine Mesenchymal Stem Cells Isolated From Bone Marrow Of Femoral Head And Subcutaneous Adipose Tissue, Nada Tanamai, Sirirak Chantakru, Monchanok Vijarnsorn
Cloning Efficiency Of Canine Mesenchymal Stem Cells Isolated From Bone Marrow Of Femoral Head And Subcutaneous Adipose Tissue, Nada Tanamai, Sirirak Chantakru, Monchanok Vijarnsorn
The Thai Journal of Veterinary Medicine
Most recently, adipose tissue (AD) has become an alternative source for mesenchymal stem cells (MSCs) instead of the invasive method of bone marrow (BM) aspiration both in human and canine. In this study, we compared MSCs derived from adipose tissue (AD-MSCs) and bone marrow (BM-MSCs) regarding morphology and cell yield for instant usage by using the standard protocol of counting colony forming unit-fibroblast (CFU-F). MSCs from both sources showed fibroblast-like morphology and formed colonies termed as CFU-F after culturing in plastic surface for 10 days. The colony number per mononuclear cells (MNCs) and the colony number per adherent cells derived …
Role Of Matrix Metalloproteinases In Animals, Sariya Asawakarn, Tanong Asawakarn
Role Of Matrix Metalloproteinases In Animals, Sariya Asawakarn, Tanong Asawakarn
The Thai Journal of Veterinary Medicine
Matrix metalloproteinases are a family of calcium-dependent and zinc-dependent endopeptidases, which excrete from many cell types. These enzymes can hydrolyze extracellular matrix proteins. The most important inhibitor of MMPs is tissue inhibitors of metalloproteinases (TIMPs). The imbalance between MMPs and TIMPs causes pathological condition in human and animals. This review focuses on structural, role and several reports of MMPs in horse, dog, cat, fish and avian. Over production of MMPs is associated with tissue destruction and erosion of cartilage in equine osteoarthritis, especially MMP-2 and MMP-9. In canine and feline, many evidences reported about MMPs and cancer such as MMP-2 …
Treatment Of Canine Transmissible Venereal Tumor Using Vincristine Sulfate Combined With L-Asparaginase In Clinical Vincristine-Resistant Cases: A Case Report, Pansawut Pansawut, Patrakrit Theewasutrakul, Sirichai Techarungchaikul, Suppawiwat Ponglowhapan, Kaywalee Chatdarong
Treatment Of Canine Transmissible Venereal Tumor Using Vincristine Sulfate Combined With L-Asparaginase In Clinical Vincristine-Resistant Cases: A Case Report, Pansawut Pansawut, Patrakrit Theewasutrakul, Sirichai Techarungchaikul, Suppawiwat Ponglowhapan, Kaywalee Chatdarong
The Thai Journal of Veterinary Medicine
Three female mongrel dogs were cytologically diagnosed as transmissible venereal tumor (TVT) and had clinically developed resistance to vincristine treatment. One dog was treated with four treatments of 10,000 IU/m2 body surface area of L-asparaginase combined with 0.025 mg/kg body weight of vincristine sulfate every two weeks while this combination was administered to the others once a week for four weeks and only vincristine sulfate once a week for four treatments. Both treatments resulted in a complete remission. Side effects such as gastrointestinal upset, diarrhea, depression, and decrease in appetite were observed in the dogs administered with the later protocol. …