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Full-Text Articles in Medicine and Health Sciences
Amyloid Beta Selectively Modulates Neuronal Trkb Alternative Transcript Expression With Implications For Alzheimer's Disease, J Wong, M Higgins, G Halliday, B Garner
Amyloid Beta Selectively Modulates Neuronal Trkb Alternative Transcript Expression With Implications For Alzheimer's Disease, J Wong, M Higgins, G Halliday, B Garner
Illawarra Health and Medical Research Institute
Dysregulation in brain-derived neurotrophic factor (BDNF)/full-length TrkB (TrkB-TK+) signaling is implicated in promoting neurodegeneration in Alzheimer's disease (AD). BDNF/TrkB-TK+ signaling can be modulated by the presence of truncated TrkB isoforms (TrkB-TK-, TrkB-Shc). All TrkB isoforms are encoded by different alternative transcripts. In this study, we assessed if expression of the three main TrkB alternative transcripts, TrkB-TK+, TrkB-TK-, and TrkB-Shc are altered in AD. Using a cohort of control and AD brains (n=29), we surveyed the hippocampus, temporal cortex, occipital cortex, and cerebellum and found specific increases in TrkB-Shc, a neuron-specific transcript, in the AD hippocampus. No significant changes were detected …
Tnf-Α Promotes Ifn-Γ-Induced Cd40 Expression And Antigen Process In Myb-Transformed Hematological Cells, Wenyi Gu, Jiezhong Chen, Lei Yang, Kong-Nan Zhao
Tnf-Α Promotes Ifn-Γ-Induced Cd40 Expression And Antigen Process In Myb-Transformed Hematological Cells, Wenyi Gu, Jiezhong Chen, Lei Yang, Kong-Nan Zhao
Illawarra Health and Medical Research Institute
Tumour necrosis factor-α, interferon-γ and interleukin-4 are critical cytokines in regulating the immune responses against infections and tumours. In this study, we investigated the effects of three cytokines on CD40 expression in Myb-transformed hematological cells and their regulatory roles in promoting these cells into dendritic cells. We observed that both interleukin-4 and interferon-γ increased CD40 expression in these hematological cells in a dose-dependent manner, although the concentration required for interleukin-4 was significantly higher than that for interferon-γ. We found that tumour necrosis factor-α promoted CD40 expression induced by interferon-γ, but not by interleukin-4. Our data showed that tumour necrosis factor-α …
Functional Expression Of The Damage-Associated Molecular Pattern Receptor P2x7 On Canine Kidney Epithelial Cells, Iman Jalilian, Mari Spildrejorde, Aine Seavers, Belinda L. Curtis, Jason D. Mcarthur, Ronald Sluyter
Functional Expression Of The Damage-Associated Molecular Pattern Receptor P2x7 On Canine Kidney Epithelial Cells, Iman Jalilian, Mari Spildrejorde, Aine Seavers, Belinda L. Curtis, Jason D. Mcarthur, Ronald Sluyter
Illawarra Health and Medical Research Institute
Epithelial cells are important in inflammation and immunity. In this study, we examined if Madin-Darby canine kidney (MDCK) epithelial cells express functional P2X7 receptors, which bind the damage-associated molecular pattern extracellular adenosine 5'-triphosphate (ATP). Reverse transcription (RT)-PCR and immunoblotting revealed the expression of P2X7 in MDCK cells. A flow cytometric assay demonstrated that ATP or 2'(3')-O-(4-benzoylbenzoyl)ATP induced ethidium(+) uptake into MDCK cells, and that this process was impaired by the P2X7 antagonists KN-62 and A438079. RT-PCR also demonstrated the presence of Toll-like receptor 4, NALP3, caspase-1, interleukin-1β and interleukin-18 in MDCK cells, as well as in positive control LPS-primed canine …