Medicine and Health Sciences Commons^™

Effects Of Atmospheric Light Scattering On Spectroscopic Observations Of Greenhouse Gases From Space. Part 2: Algorithm Intercomparison In The Gosat Data Processing For Co2 Retrievals Over Tccon Sites, Sergey Oshchepkov, Andrey Bril, Tatsuya Yokota, Paul O. Wennberg, Nicholas M. Deutscher, Debra Wunch, Geoffrey C. Toon, Yukio Yoshida, Christopher W. O'Dell, David Crisp, Charles E. Miller, Christian Frankenberg, Andre Butz, Ilse Aben, Sandrine Guerlet, Otto Hasekamp, Hartmut Boesch, Austin Cogan, Robert Parker, David Griffith, Ronald Macatangay, Justus Notholt, Ralf Sussmann, Markus Rettinger, Vanessa Sherlock, John Robinson, Esko Kyro, Pauli Heikkinen, Dietrich G. Feist, Isamu Morino, Nikolay Kadygrov, Dmitry Belikov, Shamil Maksyutov, Tsuneo Matsunaga, Osamu Uchino, Hiroshi Watanabe

Faculty of Science, Medicine and Health - Papers: part A

This report is the second in a series of companion papers describing the effects of atmospheric light scattering in observations of atmospheric carbon dioxide (CO2) by the Greenhouse gases Observing SATellite (GOSAT), in orbit since 23 January 2009. Here we summarize the retrievals from six previously published algorithms; retrieving column-averaged dry air mole fractions of CO2 (XCO2) during 22 months of operation of GOSAT from June 2009. First, we compare data products from each algorithm with ground-based remote sensing observations by Total Carbon Column Observing Network (TCCON). Our GOSAT-TCCON coincidence criteria select satellite observations within a 5° radius of 11 …

Concise Synthesis Of Α-Substituted 2-Benzofuranmethamines And Other 2-Subsituted Benzofurans Via Α-Substituted 2-Benzofuranmethyl Carbocation Intermediates, Nikhom Wongsa, Ubonta Sommart, Thunwadee Ritthiwigrom, Arife Yazici, Somdej Kanokmedhakul, Kwanjai Kanokmedhakul, Anthony C. Willis, Stephen G. Pyne

Faculty of Science, Medicine and Health - Papers: part A

Propargyl amines 4, where R3 is aryl, undergo 5-exo-dig cyclization reactions under relatively mild conditions (AgNO3, DMF, 60 °C, 1 h) to give 3-amino-2,3-dihydro-2-arylmethylidenebenzofurans 5 (R3 = aryl). In contrast, substrates where R3 is alkyl undergo competing 6-endo-dig and 5-exo-dig cyclization processes. The hydroxymethyl substrate 4 (R3 = CH2OH), however, was smoothly converted to its corresponding 5-exo-dig cyclization product 5, likely due to the assistance of the primary hydroxyl group in the 5-exo-dig cyclization process by silver cation coordination. Under more enforcing conditions (AgNO3, DMF, 100 °C, 18 h), the initially formed products 5 undergo a 1,3-allylic rearrangement to their …

Segregation Of A Latent High Adiposity Phenotype In Families With A History Of Type 2 Diabetes Mellitus Implicates Rare Obesity-Susceptibility Genetic Variants With Large Effects In Diabetes-Related Obesity, Arthur B. Jenkins, Marijka Batterham, Dorit Samocha-Bonet, Katherine Tonks, Jerry R. Greenfield, Lesley V. Campbell

Faculty of Science, Medicine and Health - Papers: part A

Background We recently reported significantly greater weight gain in non-diabetic healthy subjects with a 1st degree family history (FH+) of type 2 diabetes mellitus (T2DM) than in a matched control group without such history (FH−) during voluntary overfeeding, implying co-inheritance of susceptibilities to T2DM and obesity. We have estimated the extent and mode of inheritance of susceptibility to increased adiposity in FH+.

Methods Normoglycaemic participants were categorised either FH+ (≥1 1st degree relative with T2DM, 50F/30M, age 45±14 (SD) yr) or FH− (71F/51M, age 43±14 yr). Log-transformed anthropometric measurements (height, hip and waist circumferences) and lean, bone and fat mass …

Higher Regular Fat Dairy Consumption Is Associated With Lower Incidence Of Metabolic Syndrome But Not Type 2 Diabetes, J C. Y Louie, V M. Flood, A M. Rangan, G Burlutsky, T P. Gill, B Gopinath, P Mitchell

Faculty of Science, Medicine and Health - Papers: part A

Background and aims Limited evidence suggests habitual dairy consumption to be protective against metabolic syndrome (MetSyn) and type 2 diabetes among older adults. We assessed the association of baseline consumption of dairy products with the incidence of MetSyn and type 2 diabetes among a cohort of Australian adults aged 49 years and over. Methods and results A validated 145-item semi-quantitative food frequency questionnaire was used to assess food and nutrient intake at baseline. Ten-year incidence of MetSyn and type 2 diabetes were obtained from 1807 and 1824 subjects respectively. Odds ratios (OR) were calculated by discrete time logistic regression modelling. …

Hydroxyl Radical Formation In The Gas Phase Oxidation Of Distonic 2-Methylphenyl Radical Cations, Matthew Prendergast, Phillip A. Cooper, Benjamin B. Kirk, Gabriel Da Silva, Stephen J. Blanksby, Adam J. Trevitt

Faculty of Science, Medicine and Health - Papers: part A

The reactions of distonic 4-(N,N,N-trimethylammonium)-2-methylphenyl and 5-(N,N,N-trimethylammonium)-2-methylphenyl radical cations (m/z 149) with O 2 are studied in the gas phase using ion-trap mass spectrometry. Photodissociation (PD) of halogenated precursors gives rise to the target distonic charge-tagged methylphenyl radical whereas collision-induced dissociation (CID) is found to produce unreactive radical ions. The PD generated distonic radicals, however, react rapidly with O2 to form [M + O2]•+ and [M + O2 - OH]•+ ions, detected at m/z 181 and m/z 164, respectively. Quantum chemical calculations using G3SX(MP3) and M06-2X theories are deployed to examine key decomposition pathways of the 5-(N,N,N-trimethylammonium)-2-methylphenylperoxyl radical and rationalise …

Sequential 1,4- And 1,2-Addition Reactions To Α,Β-Unsaturated N-Acyliminium Ions: A New Strategy For The Synthesis Of Spiro And Bridged Heterocycles, Arife Yazici, Stephen G. Pyne

Faculty of Science, Medicine and Health - Papers: part A

Novel bicyclic and tetracyclic spirocycles and tricyclic bridged heterocyclic systems can be readily prepared from sequential 1,4- and 1,2-addition reactions of latent bis-nucleophiles to α,β-unsaturated N-acyliminium ions.

Comment On "Accordion Vs. Quantum Tectonics: Insights Into Continental Growth Processes From The Paleozoic Of Eastern Gondwana" By Jonathan C. Aitchison, Solomon Buckman Gondwana Research, Volume 22, Issue 2, September 2012, Pages 674-680, C L. Fergusson

Faculty of Science, Medicine and Health - Papers: part A

In regard to the tectonic history of the Lachlan Fold Belt, Aitchison and Buckman (2012) argued that "numerous aspects of the observed geology appear to be incompatible with the existing model" and that an "alternative model envisages arc-continent collision events (quantum tectonics) as a primary means of large-scale continental growth". My purpose is to challenge these arguments and to question their interpretation that rapid continental growth in the Lachlan Fold Belt has been achieved by thrusting of an island arc over a passive margin.

Protease-Activated Alpha-2-Macroglobulin Can Inhibit Amyloid Formation Via Two Distinct Mechanisms, Amy R. Wyatt, Patrick Constantinescu, Heath Ecroyd, Christopher M. Dobson, Mark R. Wilson, Janet R. Kumita, Justin J. Yerbury

Faculty of Science, Medicine and Health - Papers: part A

α2-Macroglobulin (α2M) is an extracellular chaperone that inhibits amorphous and fibrillar protein aggregation. The reaction of α2M with proteases results in an ‘activated’ conformation, where the proteases become covalently-linked within the interior of a cage-like structure formed by α2M. This study investigates, the effect of activation on the ability of α2M to inhibit amyloid formation by Aβ1–42 and I59T human lysozyme and shows that protease-activated α2M can act via two distinct mechanisms: (i) by trapping proteases that remain able to degrade polypeptide chains and (ii) by a chaperone action that prevents misfolded clients from continuing along the amyloid forming pathway.

Development And Validation Of An Insulin Proficiency Assessment Tool For Insulin Self-Management In Adults With Type 2 Diabetes Mellitus, Dianna Fornasier, Andrew Bonney

Faculty of Science, Medicine and Health - Papers: part A

No abstract provided.

Micro-Biomechanics Of The Kebara 2 Hyoid And Its Implications For Speech In Neanderthals, Ruggero D'Anastasio, Stephen Wroe, Claudio Tuniz, Lucia Mancini, Deneb T. Cesana, Diego Dreossi, Mayoorendra Ravichandiran, Marie Attard, William C. H Parr, Anne Agur, Luigi Capasso

Faculty of Science, Medicine and Health - Papers: part A

The description of a Neanderthal hyoid from Kebara Cave (Israel) in 1989 fuelled scientific debate on the evolution of speech and complex language. Gross anatomy of the Kebara 2 hyoid differs little from that of modern humans. However, whether Homo neanderthalensis could use speech or complex language remains controversial. Similarity in overall shape does not necessarily demonstrate that the Kebara 2 hyoid was used in the same way as that of Homo sapiens. The mechanical performance of whole bones is partly controlled by internal trabecular geometries, regulated by bone-remodelling in response to the forces applied. Here we show that the …

2-Cyanoethyl N,N,N',N'-Tetraisopropylphosphorodiamidite, Jichao Zhang

Faculty of Science, Medicine and Health - Papers: part A

2-Cyanoethyl N,N,N′,N′-tetraisopropylphosphorodiamidite is a colorless viscous liquid, which is soluble in most organic solvents. It is a widely used phosphitylating reagent for the preparation of various phosphorylated biomolecules, such as nucleoside carbohydrate conjugates, phospholipids and glycopeptides. In particular, this reagent is highly effective for automated solid-phase DNA/RNA oligonucleotide synthesis. 2-Cyanoethyl N,N,N′,N′-tetraisopropylphosphorodiamidite has shown great utility in the coupling of nucleobases or carbohydrates via their phosphotriesters in the presence of activators such as 1H-tetrazole, in moderate yields under mild conditions. Additionally, 2-cyanoethyl N,N,N′,N′-tetraisopropylphosphorodiamidite is cheaper and more stable than 2- cyanoethyl N,N-diisopropylchlorophosphorodiamidite, the other commonly used phosphinylating reagent. 2-Cyanoethyl N,N,N′,N′-tetraisopropylphosphorodiamidite is …

Translocation Of The Eastern Bristlebird 2: Applying Principles To Two Case Studies, Jack Baker, David Bain, Jean Clarke, Kris French

Faculty of Science, Medicine and Health - Papers: part A

The Eastern Bristlebird (Dasyornis brachypterus) is an endangered endemic passerine of south-eastern Australia. The re-establishment of extirpated populations through translocation was identified as a key action in New South Wales to address the threats to this species associated with habitat fragmentation and widespread and frequent fire. At Jervis Bay during 2003-2005, 50 birds were translocated from Bherwerre Peninsula to Beecroft Peninsula. In the Illawarra in 2008, 50 birds were translocated from Barren Grounds Nature Reserve to Cataract. At Jervis Bay, monitoring indicated that after 7 years, (i) there was no detectable impact on the source population from the removal of …

Physiological Employment Standards For Firefighters: Report 2: The Physiological Demands Of Performing Physically Demanding Fire-Fighting Duties, Nigel A.S. Taylor, Hugh Fullagar, John A. Sampson, Daniel Steven Lee, Sean Notley, Simon Burley, Herbert Groeller

Faculty of Science, Medicine and Health - Papers: part A

The aim of this research was to facilitate the identification of capable and robust recruits for Fire & Rescue New South Wales (FRNSW), such that it could sustain the capability of its workforce, whilst simultaneously minimising the risk of injury to both firefighters and members of the community.

Dietary Docosahexaenoic Acid (22:6) Incorporates Into Cardiolipin At The Expense Of Linoleic Acid (18:2): Analysis And Potential Implications, Colin Cortie, Paul L. Else

Faculty of Science, Medicine and Health - Papers: part A

Cardiolipin is a signature phospholipid of major functional significance in mitochondria. In heart mitochondria the fatty acid composition of cardiolipin is commonly viewed as highly regulated due to its high levels of linoleic acid (18:2n − 6) and the dominant presence of a 4×18:2 molecular species. However, analysis of data from a comprehensive compilation of studies reporting changes in fatty acid composition of cardiolipin in heart and liver mitochondria in response to dietary fat shows that, in heart the accrual of 18:2 into cardiolipin conforms strongly to its dietary availability at up to 20% of total dietary fatty acid and …

Beta-2 Glycoprotein I And Its Role In Antiphospholipid Syndrome: Lessons From Knockout Mice, Spyridon Miyakis, Sarah A. Robertson, S A. Krilis

Faculty of Science, Medicine and Health - Papers: part A

The antiphospholipid syndrome is characterized by the presence in serum of autoantibodies against h2GPI. Although the role of h2GPI in the pathogenesis of antiphospholipid antibody syndrome (APS) is well recognized, its exact physiological functions still remain undisclosed. Several interactions of h2GPI with components of the coagulation cascade have been proposed, resulting in both procoagulant and anticoagulant effects. Additionally, h2GPI has been implicated in the mechanism of recurrent fetal loss entailed in APS. Recently, using a homologous recombination approach, reproduction of mice homozygous for deletion of the b2GPI gene has been feasible. h2GPI knockout mice offer a valuable tool for revealing …

Fgf-2 Counteracts Loss Of Tgfβ Affected Cells From Rat Lens Explants: Implications For Pco (After Cataract), Kylie Mansfield, Anna Cerra, Coral Chamberlain

Faculty of Science, Medicine and Health - Papers: part A

Purpose: While cataract surgery initially benefits most patients, many suffer secondary loss of vision because of posterior capsule opacification (PCO). Lens epithelial cells left behind at surgery become aberrant and migrate into the light path. TGF-beta (TGFβ) appears to play a key role in this process by inducing the cells to undergo an epithelial-mesenchymal transition. Paradoxically, it also typically induces them to undergo apoptotic death. The present study was undertaken to investigate the hypothesis that FGF plays a role in PCO formation by promoting the survival of abnormal cells with PCO-like characteristics.

Methods: Rat lens epithelial explants were cultured for …

Determining Rotational Temperatures From The Oh(8-3) Band, And A Comparison With (Oh96-2) Rotational Temperatures At Davis, Antarctica, Frances Phillips, G B. Burns, W J R French, P F B Williams, A R. Klekociuk, R P. Lowe

Faculty of Science, Medicine and Health - Papers: part A

Rotational temperatures derived from the OH(8–3) band may vary by ~18K depending on the choice of transition probabilities. This is of concern when absolute temperatures or trends determined in combination with measurements of other hydroxyl bands are important. In this paper, measurements of the OH(8–3) temperature-insensitive Q/P and R/P line intensity ratios are used to select the most appropriate transition probabilities for use with this band. Aurora, airglow and solar and telluric absorption in the OH(8–3) band are also investigated. Water vapour absorption of P₁(4), airglow or auroral contamination of P₁(2) and solar absorption in the …

Beta-2 Glycoprotein1: Function In Health And Disease, Spyridon Miyakis, Bill Giannakopoulos, S A. Krilis

Faculty of Science, Medicine and Health - Papers: part A

Beta-2 glycoprotein I (β2GPI) is the principal target of autoantibodies in the antiphospholipid syndrome (APS). It is abundant in human plasma and shares high homology between different mammalian species. Although the exact physiological function of β2GPI has not been fully elucidated, several interactions have been described with other proteins and with negatively charged surfaces, such as anionic phospholipids, dextran and heparin. β2GPI is involved in the coagulation pathway, exerting both procoagulant and anticoagulant activities. Plasma from β2GPI-deficient mice exhibits impaired thrombin generation in vitro. Recently, it has been demonstrated that β2GPI binds factor (F) XI in vitro at concentrations lower …

Bovine Non-O157 Shiga Toxin 2-Containing Escherichia Coli Isolates Commonly Possess Stx2-Edl933 And/Or Stx2vhb Subtypes, Kim Brett, Michael A Hornitzky, Karl A Bettelheim, Mark J. Walker, Steven Djordjevic

Faculty of Science, Medicine and Health - Papers: part A

stx₂ genes from 138 Shiga toxin-producing Escherichia coli (STEC) isolates, of which 127 were of bovine origin (58 serotypes) and 11 of human origin (one serotype; O113:H21), were subtyped. The bovine STEC isolates from Australian cattle carried ehxA and/or eaeA and predominantly possessed stx_2-EDL933 (103 of 127; 81.1%) either in combination with stx_2vhb (32 of 127; 25.2%) or on its own (52 of 127; 40.4%). Of 22 (90.9%) bovine isolates of serotype O113:H21, a serotype increasingly recovered from patients with hemolytic uremic syndrome (HUS) or hemorrhagic colitis, 20 contained both stx_2-EDL933 and stx_2vhb; 2 …

Mesospheric Temperatures From Observations Of The Hydroxyl (6-2) Emission Above Davis, Antarctica: A Comparison Of Rotational And Doppler Measurements, J L. Innis, Frances Phillips, G B. Burns, P A. Greet, W J R French, P L. Dyson

Faculty of Science, Medicine and Health - Papers: part A

We present observations of the hydroxyl (6–2) airglow lines from ~ 87 km altitude obtained at Davis station, Antarctica, in the austral winter of 1999. Nine nights of observations were made of the P-branch near λ840 nm with a Czerny-Turner scanning spectrometer (CTS); at the same time, high-resolution Fabry-Perot Spectrometer (FPS) spectra were collected of the Q₁(1) doublet at λ834 nm. Rotational temperatures were determined from the CTS observations, while Doppler temperatures were derived from the line-widths of the FPS Q₁(1) spectra. Absolute temperatures determined by these methods are uncertain by ~ 2 and ~ 20 …

The Common Ovine Shiga Toxin 2-Containing Escherichia Coli Serotypes And Human Isolates Of The Same Serotypes Possess A Stx2d Toxin Type, Vidiya Ramachandran, Michael A. Hornitzky, Karl A. Bettelheim, Mark J. Walker, Steven P. Djordjevic

Faculty of Science, Medicine and Health - Papers: part A

Shiga toxin 2 (Stx2) has been reported as the main Shiga toxin associated with human disease. In addition, the Stx2 toxin type can have a profound impact on the degree of tissue damage in animal models. We have characterized the stx₂ subtype of 168 Shiga toxin-producing Escherichia coli (STEC) isolates of which 146 were derived from ovine sources (principally feces and meat) and 22 were isolated from humans. The ovine STEC isolates were of serotypes that have been shown to occur commonly in the gastrointestinal tract of healthy sheep. The major stx₂ subtype in the ovine isolates was …

Physical Activity, Change In Blood Pressure And Predictors Of Mortality In Older South Africans - A 2-Year Follow-Up Study, Karen E. Charlton, Estelle V. Lambert, Judith Kreft

Faculty of Science, Medicine and Health - Papers: part A

Objective. A 2-year follow-up study of a cohort of 200 historically disadvantaged older South Africans was conducted to: (i) characterise current levels of habitual physical activity; (ii) relate physical activity to current risk factors for chronic disease; and (iii) identify risk factors associated with 2-year mortaJity. The baseline sample, drawn in 1993, was found to have a high prevalence of hypertension (71.7%). Research design. Retrospective cohort study. Methods. A baseline sample of 200 persons aged ;:;.. 65 years, resident in the Cape Peninsula, was randomly drawn by means of a two-stage cluster design. Baseline measurements included: anthropometry, waist/hip ratio, systolic …