Medicine and Health Sciences Commons^™

Single-Channel Properties In Endoplasmic Reticulum Membrane Of Recombinant Type 3 Inositol Trisphosphate Receptor., D O Mak, S Mcbride, V Raghuram, Y Yue, Suresh K. Joseph, J K Foskett

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

The inositol 1,4,5-trisphosphate receptor (InsP(3)R) is an intracellular Ca(2+)-release channel localized in endoplasmic reticulum (ER) with a central role in complex Ca(2+) signaling in most cell types. A family of InsP(3)Rs encoded by several genes has been identified with different primary sequences, subcellular locations, variable ratios of expression, and heteromultimer formation. This diversity suggests that cells require distinct InsP(3)Rs, but the functional correlates of this diversity are largely unknown. Lacking are single-channel recordings of the recombinant type 3 receptor (InsP(3)R-3), a widely expressed isoform also implicated in plasma membrane Ca(2+) influx and apoptosis. Here, we describe functional expression and single-channel …