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Dengue Virus-Specific Murine T-Lymphocyte Proliferation: Serotype Specificity And Response To Recombinant Viral Proteins, Alan L. Rothman, Ichiro Kurane, Yi-Ming Zhang, Ching-Juh Lai, Francis A. Ennis

Alan Rothman

Definition of the T-lymphocyte responses to dengue viruses should aid in the development of safe and effective vaccines and help to explain the pathophysiology of dengue hemorrhagic fever and dengue shock syndrome. In this study, we demonstrated that dengue virus-specific T lymphocytes were detected in spleen cells from dengue virus-immune mice using an in vitro proliferation assay. Following immunization with a single dose of infectious dengue virus, murine lymphocytes showed increased proliferation when incubated in the presence of viral antigens of the same serotype but not in the presence of control antigens. Depletion experiments with antibody and complement showed that …