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Anti-Müllerian Hormone Immunolocalization In Growing Follicles Of Replacement Gilts Culled Due To Anestrus, Paisan Tienthai, Padet Tummaruk
Anti-Müllerian Hormone Immunolocalization In Growing Follicles Of Replacement Gilts Culled Due To Anestrus, Paisan Tienthai, Padet Tummaruk
The Thai Journal of Veterinary Medicine
In female pigs, the anti-Müllerian hormone (AMH) produced by granulosa cells of developing follicles plays an important role in ovarian reserves of follicles and serum AMH levels are the biological marker of gilt reproductive performance. In the present study, we determined the AMH expression during follicular growth in replacement gilts culled due to anestrus by immunohistochemical technique. A total of 20 reproductive organs from crossbred Landrace Yorkshire gilts were collected from swine commercial farms in Thailand and categorized by the reasons for culling, i.e., the anestrus group (n=12) and other causes not related to reproduction as the control group …
Morphological Considerations Of The Reproductive Organs In Anestrus Gilts Affiliated With True Hermaphrodites In Thailand, Paisan Tienthai, Sawang Kesdangsakonwut, Padet Tummaruk
Morphological Considerations Of The Reproductive Organs In Anestrus Gilts Affiliated With True Hermaphrodites In Thailand, Paisan Tienthai, Sawang Kesdangsakonwut, Padet Tummaruk
The Thai Journal of Veterinary Medicine
True hermaphrodites are animals with both male and female gonads that have concurrently developed in the same individual. The incidence of true hermaphroditism is moderately higher in pigs than in other domestic animals. The aim of this study was to describe the genital organs of gilts culled due to anestrus with true hermaphrodites by gross and histological analysis. Three anestrus gilts were categorized as unilateral true hermaphrodites, having one ovotestis and one ovary, while three other gilts were bilateral true hermaphrodites with two ovotestes. The anomalous structures of the female genital organs, such as oviductal aplasia and hyperclitoris were found …
Recent Antimicrobial Susceptibility Of Lawsonia Intracellularis Field Isolates From Pigs With Proliferative Hemorrhagic Enteropathy In Korea, Byoung-Joo Seo, Sung-Hyun Moon, So-Min Lee, Seung-Yoon Lee, Byeong-Yeal Jung, Won-Il Kim, Chang-Seop Lee, Yeonsu Oh, Ho-Seong Cho
Recent Antimicrobial Susceptibility Of Lawsonia Intracellularis Field Isolates From Pigs With Proliferative Hemorrhagic Enteropathy In Korea, Byoung-Joo Seo, Sung-Hyun Moon, So-Min Lee, Seung-Yoon Lee, Byeong-Yeal Jung, Won-Il Kim, Chang-Seop Lee, Yeonsu Oh, Ho-Seong Cho
The Thai Journal of Veterinary Medicine
The study was performed to investigate the antimicrobial susceptibility of L. intracellularis field isolates from Korean pig farms. The novel four field isolates successfully obtained from clinical cases were prepared in IEC-18 cells to conduct the in vitro antimicrobial susceptibility testing (AST) by determining minimum inhibitory concentrations (MIC)s; either intracellular (InMIC) or extracellular MICs (ExMIC). The final MICs were assessed by counting the number of heavily infected cells (HICs;> 30 bacteria per cell) using an immunoperoxidase monolayer assay. Enrofloxacin (0.125 to 0.25 μg/mL: InMIC and 2 to 16 μg/mL; ExMIC) presented the most notable antimicrobial susceptibility, and marbofloxacin (0.25 to …
Plasmid Profile Of Enterococcus Faecium And Enterococcus Faecalis Isolated From Pigs, Pork And Humans In Thai-Laos Border Provinces, Wink Phyo Thu, Nuananong Sinwat, Rungtip Chuanchuen
Plasmid Profile Of Enterococcus Faecium And Enterococcus Faecalis Isolated From Pigs, Pork And Humans In Thai-Laos Border Provinces, Wink Phyo Thu, Nuananong Sinwat, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
This study aimed to determine plasmid profile of E. faecium and E. faecalis isolated from pigs, pig products and humans in Thailand-Laos border provinces. A total of 96 Enterococcus spp. including E. faecium (n=59) and E. faecalis (n=37) from pigs, pork and humans were included. All were determined for their antimicrobial susceptibilities and plasmid profile. Most isolates (96.9%) carried one to four plasmids with a molecular weight of 0.03-35 kb in E. faecium and 19-40 kb in E. faecalis. The 26 kb plasmid was most commonly detected. Variable plasmid profiles were defined, of which the most common pattern was, 23 …
Management Strategies In Farrowing House To Improve Piglet Pre-Weaning Survival And Growth, Ramon Muns Vila, Padet Tummaruk
Management Strategies In Farrowing House To Improve Piglet Pre-Weaning Survival And Growth, Ramon Muns Vila, Padet Tummaruk
The Thai Journal of Veterinary Medicine
Post-partum and lactation are the most complex periods in the swine production chain. Newborn pigs are highly vulnerable due to relatively low body weight at birth and physiological immaturity. Most of the management strategies performed in farrowing houses are oriented to ensure a proper level of colostrum intake by the piglets. Colostrum is essential as an energy source and to provide passive immunity to piglets. Different farrowing supervising protocols have been comprehensively investigated to reduce early mortality as well as to assist newborn piglets in obtaining an optimal amount of colostrum and milk. However, little is known of the benefits …
Prevalence Of Porcine Sapovirus In Suckling Piglets In Hunan Province, China, Sheng-Guo Tan, Na Liu, Ping-Yuan Xu
Prevalence Of Porcine Sapovirus In Suckling Piglets In Hunan Province, China, Sheng-Guo Tan, Na Liu, Ping-Yuan Xu
The Thai Journal of Veterinary Medicine
Sapoviruses (SaVs) are emerging enteric pathogens that cause diarrhea in humans and animals. In the current study, genetic identification of sapovirus (SaV) in suckling piglets was investigated in Hunan province, China between May 2013 and May 2014. Diarrheic fecal (n=300) samples collected from suckling piglets in Hunan province, China were evaluated using RT-PCR and then sequenced. Overall, 45 of the 300 (15%) samples were found to contain porcine SaV. In addition, phylogenetic analysis showed that all the porcine SaV isolates belonged to the porcine SaV genogroup III (GIII). The results of the present investigation have implications for the control of …
Importance Of Backfat Thickness To Reproductive Performance In Female Pigs, Atthaporn Roongsitthichai, Padet Tummaruk
Importance Of Backfat Thickness To Reproductive Performance In Female Pigs, Atthaporn Roongsitthichai, Padet Tummaruk
The Thai Journal of Veterinary Medicine
Backfat in pigs consists of water, collagen, and lipid. Apart from age, body weight, and number of estrus expression, backfat thickness is one of the significant parameters to consider when selecting female pigs into breeding herds since it dominates a number of reproductive performances, e.g. puberty attainment, total piglets born (TB), and farrowing rate. Besides, backfat is one of the significant sources of hormones related to puberty attainment, such as leptin, insulin-like growth factor-I (IGF-I), and progesterone (P4). Evaluation of backfat thickness is majorly performed by an A-mode ultrasonography at P2 position; it provides more accurate body condition than visual …
Rapid Single Cell Typing Using Sybr® Green Real-Time Pcr Together With Melt Curve Analysis For Sex Identification Of Porcine Sperm, Varaporn Korchunjit, Kampon Kaeoket, Yindee Kitiyanant, Tuempong Wongtawan
Rapid Single Cell Typing Using Sybr® Green Real-Time Pcr Together With Melt Curve Analysis For Sex Identification Of Porcine Sperm, Varaporn Korchunjit, Kampon Kaeoket, Yindee Kitiyanant, Tuempong Wongtawan
The Thai Journal of Veterinary Medicine
Identification of X or Y chromosome is a very useful technique to verify the sex of boar sperm, but common methods used in pig such as Fluorescence In situ Hybridisation (FISH) and whole semen Polymerase Chain Reaction (PCR) have some limitations. FISH is highly accurate, but time-consuming (>3 days). Whole semen PCR is faster than FISH (3-6 h), but not highly accurate (approximate methods). The objective of this study was to develop a fast and highly accurate protocol to identify sex of boar sperm. In the present study, our team developed an alternative sex identification protocol using single cell …
Enhancing Specific Antibodies Using A Developed Sub-Unit Pcv2b Vaccination In A Pcv2-Affacted Herd, Suphattra Jittimanee, Suparlark Nuntawan Na Ayudhya, Roongtham Kedkovid, Komkrich Teankum, Sanipa Suradhat, Roongroje Thanawongnuwech
Enhancing Specific Antibodies Using A Developed Sub-Unit Pcv2b Vaccination In A Pcv2-Affacted Herd, Suphattra Jittimanee, Suparlark Nuntawan Na Ayudhya, Roongtham Kedkovid, Komkrich Teankum, Sanipa Suradhat, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Porcine circovirus type 2 (PCV2) causes multisystemic diseases called porcine circovirus associated disease (PCVAD) in pigs leading to a significant economic loss in the swine industry. The objective of this study was to determine the efficacy of a developed sub-unit PCV2b vaccine using a recombinant truncated capsid protein expressed in Escherichia coli in PCVAD-affected pig farms. Five-week-old conventional pigs were allocated into 2 groups: vaccinated (n = 55) and control (n = 55) groups placed in the same building. The vaccinated pigs were given two shots of a developed vaccine with 2-week interval on day 0 and 14. Serum samples …
Association Among Serum Insulin–Like Growth Factor–I, Backfat Thickness, And Age At First Observed Estrus In Gilts, Atthaporn Roongsitthichai, Seri Koonjaenak, Padet Tummaruk
Association Among Serum Insulin–Like Growth Factor–I, Backfat Thickness, And Age At First Observed Estrus In Gilts, Atthaporn Roongsitthichai, Seri Koonjaenak, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The present study investigated the association among serum insulin-like growth factor-I (IGF-I), backfatthickness, and age at first observed estrus in replacement gilts. In total, 80 replacement gilt, Landrace (L, n = 48) andYorkshire (Y, n = 32), were included. The gilts were determined for age at first observed estrus between 167 to 212days of age. They were categorized according to age at first observed estrus into ≤ 200 days (n = 41) and > 200 days (n= 36) groups. Backfat thickness, body weight, and serum IGF–I concentration were measured before mating. Age atfirst mating, interval from boar exposure to first observed …
Development Of Semi-Nested Pcr For Detection Of 16s Rrna Gene Of Mycoplasma Hyosynoviae, Pacharee Thongkamkoon, Watcharachai Narongsak, Metta Makhanon, Kobayashi Hideki, Koshi Yamamoto
Development Of Semi-Nested Pcr For Detection Of 16s Rrna Gene Of Mycoplasma Hyosynoviae, Pacharee Thongkamkoon, Watcharachai Narongsak, Metta Makhanon, Kobayashi Hideki, Koshi Yamamoto
The Thai Journal of Veterinary Medicine
In Thailand, some breeders in pig farms have had osteoarthritis lesions for which M. hyosynoviae might be one of the causative agents. Therefore, the semi-nested polymerase chain reaction assay was developed for detection of Mycoplasma hyosynoviae in organs of pigs in Thailand using three oligonucleotide primers specific to 16S rRNA gene of M. hyosynoviae. The detection limit of purified DNA was 10 femtogram per reaction and of the simulated lung sample was 103 CFU per gram of sample. Preliminary study for tonsil-carrier state of M. hyosynoviae in slaughtered pigs revealed the presence of M. hyosynoviae in 5 out of 10 …
Assessment Of Appropriate L-Cysteine Concentration For Boar Semen Cryopreservation By Using Flow Cytometry, Paisan Tienthai, Panida Chanapiwat, Sukanya Manee-In, Dulyatad Gronsang, Kampon Kaeoket
Assessment Of Appropriate L-Cysteine Concentration For Boar Semen Cryopreservation By Using Flow Cytometry, Paisan Tienthai, Panida Chanapiwat, Sukanya Manee-In, Dulyatad Gronsang, Kampon Kaeoket
The Thai Journal of Veterinary Medicine
The present study aims to investigate a suitable L-cysteine level to be added the lactose-egg yolk (LEY) extender to improve the quality of boar semen after freezing-thawing using Annexin-V and propidium iodide (PI) assay detected with flow cytometer. Ejaculates from twelve boars were processed in the LEY extender and added with 0, 5, 10 and 15 mM of L-cysteine. Semen suspensions were cryopreserved with a controlled-rate freezer and thawed for examination. The surface of sperm plasma membrane was explored by scanning electron microscopy (SEM) while sperm membrane integrity was performed by Annexin-V/PI assay. Of all treatments, the damaged spermatozoa in …
A Comparative Study On The Efficacy Of Four Semen Extenders And Thawing By Seminal Plasma On The Quality Of Frozenthawed Boar Semen, Chonthida Rienprayoon, Chonlapat Klangnak, Suttiporn Onton, Chanyuth Tretipskul, Padet Tummaruk
A Comparative Study On The Efficacy Of Four Semen Extenders And Thawing By Seminal Plasma On The Quality Of Frozenthawed Boar Semen, Chonthida Rienprayoon, Chonlapat Klangnak, Suttiporn Onton, Chanyuth Tretipskul, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The present study investigated the influence of four different commercial semen extenders used during theholding time and the use of seminal plasma as a thawing medium on the frozen-thawed (FT) boar semen quality.Sperm-rich fractions were collected from 11 boars and were divided into four groups according to the type of semenextender used during the holding time (A, B, C and D). The extended semen was cooled down and centrifuged. Thesperm pellets were diluted in lactose-egg yolk extender plus glycerol and Equex STM Paste. The semen were loadedinto 0.5 ml straws and frozen. Thawing was achieved by immersing the straws in …
Influenza A Virus Receptor Identification In The Respiratory Tract Of Quail, Pig, Cow And Swamp Buffalo, Aunyarattana Thontiravong, Tilladit Rung-Ruangkijkrai, Pravina Kitikoon, Kanisak Oraveerakul, Yong Poovorawan
Influenza A Virus Receptor Identification In The Respiratory Tract Of Quail, Pig, Cow And Swamp Buffalo, Aunyarattana Thontiravong, Tilladit Rung-Ruangkijkrai, Pravina Kitikoon, Kanisak Oraveerakul, Yong Poovorawan
The Thai Journal of Veterinary Medicine
Virus infection requires the interaction of a virus protein with the host-cell receptor. The Influenza A virusreceptor consists of sialic acid linked to the galactose unit in a α 2,3 or α 2,6 conformation. Various types of thesereceptors are expressed differently on the epithelial lining of various animal species. Here we characterized the typesof receptors that are expressed in the upper and lower respiratory tracts of cow, buffalo, pig and quail. Our findingsdemonstrate that SA α 2,6-gal linked receptors for human influenza viruses are present in the lower respiratory tractof cow and buffalo, while the SA α 2,3-gal linked receptors …
Prevalence And Genotype Of Salmonella Choleraesuis In Gunma Prefecture, Japan, Chieko Sakano, Yukio Morita, Koichi Goto, Yoko Yokota, Hideyuki Annaka, Masahiro Fujita, Satoshi Kobatake, Taisei Ishioka, Toshie Hoshino, Sumalee Boonmar, Chaiwat Pulsrikarn, Atsuyoshi Nishina, Kunihisa Kozawa, Shigeki Yamamoto, Hirokazu Kimura
Prevalence And Genotype Of Salmonella Choleraesuis In Gunma Prefecture, Japan, Chieko Sakano, Yukio Morita, Koichi Goto, Yoko Yokota, Hideyuki Annaka, Masahiro Fujita, Satoshi Kobatake, Taisei Ishioka, Toshie Hoshino, Sumalee Boonmar, Chaiwat Pulsrikarn, Atsuyoshi Nishina, Kunihisa Kozawa, Shigeki Yamamoto, Hirokazu Kimura
The Thai Journal of Veterinary Medicine
We studied the prevalence of swine salmonellosis and PFGE genotype of isolates in Gunma Prefecture,Japan. Between 2005 and 2008, swine salmonellosis was confirmed in 430 of 2,707,402 (0.02%) swine atslaughterhouses. All isolates were identified as deriving from Salmonella Choleraesuis, biotype Choleraesuis (negativefor H2S production). We used 30 bacterial strains from 15 farms that had experienced outbreaks in 2006 and 2007. Allstrains were susceptible to various antibiotics such as cephems (cefotaxime), fluoroquinolones (norfloxacin andciprofloxacin), and fosfomycin. On the other hand, all strains were resistant to tetracycline (TC), and 29 of 30 (97%)strains were resistant to streptomycin (SM). The most predominant profiles …
Improvement Of Normal Fertilization Rate And Embryo Development By Reduction Of Sperm: Oocyte Ratio During In Vitro Fertilization In Pig, Sasithorn Panasophonkul, Theerawat Tharasanit, Panida Chanapiwat, Mongkol Techakumphu
Improvement Of Normal Fertilization Rate And Embryo Development By Reduction Of Sperm: Oocyte Ratio During In Vitro Fertilization In Pig, Sasithorn Panasophonkul, Theerawat Tharasanit, Panida Chanapiwat, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
The present study was undertaken to determine the influence of sperm:oocyte ratio at fertilization in vitrousing frozen boar semen on fertilization rates and subsequent embryo development and quality in pig. Cumulusoocytecomplexes (COCs) were collected from porcine ovaries and then matured in vitro. After 44 hours of culture,matured oocytes were fertilized for 6 hours with three different sperm:oocyte ratios (1000:1, 2000:1, and 4000:1).Presumptive zygotes were fixed at 18-20 hours post-fertilization and then examined for sperm penetration andmonospermy rates. The developmental competence, in terms of cleavage and blastocyst rates, and the blastocystquality were evaluated at 48 and 168 hours post-fertilization, respectively. Sperm …
Comparative Study Of Temperatures Used In Silicone Impregnation Of Porcine Hearts Plastination, Damri Darawiroj, Adisorn Adirekthaworn, Sayamon Srisuwattanasakul, Kongkiat Srisuwattanasakul
Comparative Study Of Temperatures Used In Silicone Impregnation Of Porcine Hearts Plastination, Damri Darawiroj, Adisorn Adirekthaworn, Sayamon Srisuwattanasakul, Kongkiat Srisuwattanasakul
The Thai Journal of Veterinary Medicine
Forced impregnation process is normally performed under vacuum at freezing temperature which requires expensive plastination apparatus. To reduce the cost in the plastination process, forced impregnation of porcine hearts under two different environments, which are at the freezing temperature (-20oC) and at room temperature (22-25oC), was compared. Twelve porcine hearts were collected from a slaughter house, and divided into the groups, six in each, and subjected to be plastinated by S10 standard technique at the two temperatures. Although the forced impregnation at room temperature, also the curing process, was more time-consuming, all hearts from both groups produced satisfying plastinated specimens. …
Establishment Of Porcine Embryonic Stem-Like Cells From Parthenogenetic And In Vivo Derived Embryos, Sasithorn Panasophonkul, Theerawat Tharasanit, Mongkol Techakumphu
Establishment Of Porcine Embryonic Stem-Like Cells From Parthenogenetic And In Vivo Derived Embryos, Sasithorn Panasophonkul, Theerawat Tharasanit, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
The study was conducted to examine the effect of different parthenogenetic activation (PA) protocols on developmental competence and quality of embryos, and to establish the parthenogenetic embryonic stem (ptES)-like cells in pigs. Compacted cumulus-oocyte complexes (COCs) were collected from porcine ovaries and then cultured in M199-medium. After 44 hrs of culture, matured MII-oocytes were selected and divided into three groups. Oocytes in each group were stimulated with three different activation protocols as protocol I: 1.36 kV/cm, 30 μsec, 2 pulses; protocol II: 1.50 kV/cm, 60 μsec, 2 pulses and protocol III: 1.0 kV/cm, 80 μsec, 3 pulses, and followed by …
The Sperm Dna Damage After Cryopreservation Of Boar Semen In Relation To Post-Thawed Semen Qualities, Antioxidant Supplementation And Boars Effects, Panida Chanapiwat, Kampon Kaeoket, Padet Tummaruk
The Sperm Dna Damage After Cryopreservation Of Boar Semen In Relation To Post-Thawed Semen Qualities, Antioxidant Supplementation And Boars Effects, Panida Chanapiwat, Kampon Kaeoket, Padet Tummaruk
The Thai Journal of Veterinary Medicine
The objectives of the present study were to evaluate the damage of DNA of the frozen-thawed (FT) boar spermatozoa and to investigate the effect of various concentrations of L-cysteine supplementation on the sperm DNA damage. A total of 104 cryopreserved semen samples from twenty-six ejaculates of 16 proven boars were analyzed. Of these samples, each semen sample contained a different concentration of L-cysteine i.e., 0 (n=41), 5 (n=41), 10 (n=11) and 15 (n=11) mM. All of the semen samples were cryopreserved by controlled-rate freezer. The semen was thawed at 50oC for 12 sec and the damage to the sperm DNA …
Detection Of Antibody Titer Against Toxoplasma Gondii In Aborted Sows And Gilts In A Swine Herd In Thailand, Padet Tummaruk, Woraporn Sukhumavasi, Sasiwimon Talummuk, Annop Kunavongkrit
Detection Of Antibody Titer Against Toxoplasma Gondii In Aborted Sows And Gilts In A Swine Herd In Thailand, Padet Tummaruk, Woraporn Sukhumavasi, Sasiwimon Talummuk, Annop Kunavongkrit
The Thai Journal of Veterinary Medicine
No abstract provided.
Prevention And Control Of Respiratory Diseases Excluding Mycoplasma Hyopneumoniae, Stanley Done, David Burch
Prevention And Control Of Respiratory Diseases Excluding Mycoplasma Hyopneumoniae, Stanley Done, David Burch
The Thai Journal of Veterinary Medicine
Significant changes in respiratory disease have occurred over the last 20 years. There are two primary reasons for this. In the first instance, there has been the occurrence of two major worldwide plagues-PRRS and PCV2-associated diseases. The second reason has been the considerable advances in vaccination, which have become available for some of the respiratory diseases, in particular Mycoplasma hyopneumoniae. Vaccines have also become available for porcine reproductive and respiratory syndrome virus (PRRS), influenza viruses (outside UK) and probably in the near future against porcine circovirus type 2 (PCV2).
Strategies For The Control Of Respiratory Disease, Stanley Done
Strategies For The Control Of Respiratory Disease, Stanley Done
The Thai Journal of Veterinary Medicine
This is a paper about common sense and respiratory disease using information that has been known for some time i.e. historical, and which is not accessed when a literary search is supplied by a computer. Respiratory disease in pigs has its most serious effects in the finishing stages, when the sequelae to progressive atrophic rhinitis, enzootic pneumonia and Actinobacillosis are most severe. Nowadays this relatively simple position is even more complicated by the worldwide occurrence of porcine reproductive and respiratory syndrome (PRRS) and porcine circovirus type 2 (PCV-2)-associated diseases principally PMWS where no vaccination is practised. Up to 80% of …
Pathological Study Of Helicobacter Spp. Infection In Pig Stomachs, Nopadon Pirarat, Veerasak Sada, Supradit Wangnaitham, Boonmee Sunyasootcharee
Pathological Study Of Helicobacter Spp. Infection In Pig Stomachs, Nopadon Pirarat, Veerasak Sada, Supradit Wangnaitham, Boonmee Sunyasootcharee
The Thai Journal of Veterinary Medicine
pathological study of Helicobacter spp. infection in pigs was performed to determine the incidence of Helicobacter spp. infection, and to define the relationship between Helicobacter spp. infection and the histopathological changes of gastric mucosa in pigs, using Warthin Starry stain and immunohistochemistry. A total of 115 biopsies of the cardia, fundus, body and pyloric antrum of swine (mean weight 150 kg) obtained from slaughterhouses in Nakornpathom province (Thailand) were investigated. Histopathological results of the cardia region showed thickening of mucosa 54.78% (63/115), vacuolated degeneration of the epithelium 83.47% (96/115), lymphoid aggregation 74.78% (86/115), inflammatory cells infiltration 38.26% (44/115) and neovascularized …
Morphological Changes In The Oviduct Of Culling Replacement Gilts, Paisan Tienthai, Kriengyot Sajjarengpong, Padet Talummuk
Morphological Changes In The Oviduct Of Culling Replacement Gilts, Paisan Tienthai, Kriengyot Sajjarengpong, Padet Talummuk
The Thai Journal of Veterinary Medicine
The aim of this study was to investigate the morphological changes of the oviduct from 30 culling replacement gilts and 6 control sows. Blood samples and female genital organs were taken immediately after slaughter. Post-mortem examinations were made on the genital organs and oviductal samples, i.e. utero-tubal junction (UTJ), isthmus and ampulla, were collected and fixed for histological analysis. Observations indicated that the reasons for culling the gilts were 'no estrus' (68%) and 'repeat breeding' (32%). The greatest pathological changes (67%) were found in repeat breeding gilts, whereas 52% of no estrous gilts were at prepubertal phase. The female hormonal …
Relationships Between The Immune System And Stress Reactivity In Pig: Visualizing The Immuno-Neuroendocrine Framework In Action, Sanipa Suradhat
Relationships Between The Immune System And Stress Reactivity In Pig: Visualizing The Immuno-Neuroendocrine Framework In Action, Sanipa Suradhat
The Thai Journal of Veterinary Medicine
Activation of the immune system is regulated through several mechanisms, within the system itself and by other body systems, in order to maintain disease resistance, and prevent immune-mediated disorders within the host. It is well accepted that the immunological, physiological, behavioral, and psychological state of an animal are closely linked and the factors affecting one feature of the network can influence the rest of the system.In recent years, our understanding of the interactions between the neuroendocrine network (i.e. the hypothalamicp tuitary- adrenal axis, HPA) and immune-mediated inflammatory reactions has expanded enormously. This article outlines the nature of the immuno-neuroendocrine network …
An Immunolohistochemical Study Of Anti-Apoptotic Bcl-2 In The Pig’S Oviduct During The Oestrous Cycle, Paisan Tienthai
An Immunolohistochemical Study Of Anti-Apoptotic Bcl-2 In The Pig’S Oviduct During The Oestrous Cycle, Paisan Tienthai
The Thai Journal of Veterinary Medicine
The purpose of this study was to determine the localization of Bcl-2 protein in porcine oviducts. Anti-human Bcl-2 monoclonal antibody was used to localize Bcl-2 protein immunohistochemically in the uterotubal junction (UTJ), isthmus and ampulla of pre-ovulatory (n=10) and post-ovulatory (n=10) sows. The Bcl-2 protein was detected in quantity in the intracytoplasm of the epithelial cells, some types of leukocytes in the epithelial layer and in the subepithelial connective tissue, in fibroblasts, as well as smooth muscle cells of the UTJ, isthmus and ampulla in the pre-ovulatory period. In contrast, the expression of Bcl-2 was lower in the oviduct tissues …
Intrauterine Artificial Insemination In The Pig: The Techniques, The Applications And The Limitations, Tuempong Wongtawan, Peerapong Samransarp, Padet Tummaruk
Intrauterine Artificial Insemination In The Pig: The Techniques, The Applications And The Limitations, Tuempong Wongtawan, Peerapong Samransarp, Padet Tummaruk
The Thai Journal of Veterinary Medicine
This review aims to update information on the success of intra-uterine and deep intra-uterine insemination in the pig. Three artificial insemination (AI) techniques in pigs included conventional AI (semen is deposited intra cervically), intra-uterine insemination (IUI) and deep intra-uterine insemination (DIUI). With conventional AI, it has been shown that more than 90% of spermatozoa are lose before reaching the oviducts. Therefore, new techniques for AI were developed in order to maximize the use of boar semen and to reduce semen backflow, which can reduce pregnancy rates. The advantages of intra-uterine and deep intra-uterine insemination include a reduction in the number …
Hyaluronan In The Sow’S Oviduct: Its Effect On Boar Sperm Morphology And Function, Paisan Tienthai
Hyaluronan In The Sow’S Oviduct: Its Effect On Boar Sperm Morphology And Function, Paisan Tienthai
The Thai Journal of Veterinary Medicine
During sperm transport in the female pig, a proportion of the spermatozoa is arrested at the uterotubal junction and the isthmus, where an oviductal sperm reservoir is built up. The sperm reservoir maintains sperm viability and controls the release of fertilizing spermatozoa so that only a small subpopulation reaches the site of fertilization, thus diminishing the risk of polyspermy. In vitro research has focused on sperm binding as the main mechanism of sperm storage, sperm release and modulation of capacitation, but little attention has been paid to the sperm reservoir fluid, its composition and the control of capacitation in vivo. …
Fas Ligand Expression In The Porcine Oviduct, Paisan Tienthai, Yumi Hoshino
Fas Ligand Expression In The Porcine Oviduct, Paisan Tienthai, Yumi Hoshino
The Thai Journal of Veterinary Medicine
The expression of Fas/Fas Ligand (FasL) is thought to be involved in the immune privileged status of the testis, cornea and the placenta. The objective of this study was to see whether FasL mRNA was expressed in the epithelium of the porcine oviduct, where spermatozoa, oocytes and developing embryos avoid elimination by the female immune system during the estrous cycle. The distribution of Fas/FasL might provide information about immune privileged sites in the pig oviduct. The gene expression of FasL was detected in the uterotubal junction (UTJ), isthmus and ampulla of the pig oviductal epithelium, during both pre- and post-ovulatory …
Ultrastructural Changes In Porcine Oocytes After Ultrarapid Freezing, Adisorn Adirekthaworn, Kriengyot Sajjarengpong, Siripen Vethchagarun, Wimon Pothiwong, Mongkol Techakumphu
Ultrastructural Changes In Porcine Oocytes After Ultrarapid Freezing, Adisorn Adirekthaworn, Kriengyot Sajjarengpong, Siripen Vethchagarun, Wimon Pothiwong, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
The objective of the present study was to investigate the ultrastructural changes seen in frozen porcine oocytes at the germinal vesicle and during in vitro maturation when using an ultrarapid freezing technique. The morphological changes were assessed by transmission electron microscopy (TEM). Immature oocytes were collected from pig ovaries at a local slaughter house in Nakorn Prathom province. Some oocytes were matured in vitro for 44 hours at 39oC, 5% CO2 with high humidity. All oocytes were divided into four groups; immature (control group), the in vitro maturation group (control group), frozen immature and frozen mature oocytes. Electron microscopy was …