Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 5 of 5
Full-Text Articles in Medicine and Health Sciences
Serological Analysis Of Equine Antibody Responses To The Facultative Intracellular Bacteria Corynebacterium Pseudotuberculosis, Roberta Pollock, Dina Abdel-Massih, Luca Valle, Alexis Buchnam, Sharon Spier
Serological Analysis Of Equine Antibody Responses To The Facultative Intracellular Bacteria Corynebacterium Pseudotuberculosis, Roberta Pollock, Dina Abdel-Massih, Luca Valle, Alexis Buchnam, Sharon Spier
Roberta Pollock
Corynebacterium pseudotuberculosis is a gram positive facultative intracellular bacteria that causes pigeon fever or dryland distemper in horses. Most infected horses develop large pus-filled external abscesses, which resolve with a low mortality rate. Some infected horses develop internal abscesses, and about 40% of these animals die. Our hypothesis is that the TH1 toTH2 balance determines the form of the disease: TH1 dominance leads to external abscesses and TH2 dominance leads to internal abscesses. We developed an ELISA that measures the antibody response to phospholipase D (PLD), the major antigen and exotoxin of the bacteria, and screened 50 infected and uninfected …
The Molecular And Biochemical Characterization Of Mutant Monoclonal Antibodies With Increased Antigen Binding, Roberta Pollock, Deborah French, Hector Aguila, Matthew Scharff
The Molecular And Biochemical Characterization Of Mutant Monoclonal Antibodies With Increased Antigen Binding, Roberta Pollock, Deborah French, Hector Aguila, Matthew Scharff
Roberta Pollock
Mutant mAb with increased Ag binding were generated from a hybridoma cell line, 36-65, that secretes an IgG1,kappa anti-p-azophenylarsonate-(Ars) specific antibody. The mutant antibodies were identified using an Ars-specific ELISA and sib selection so that approximately 10(6) cells could be analyzed. The ELISA used as Ag a low ratio of Ars coupled to BSA and was set up so that only those antibodies that had higher binding than the parent would be detected. Seven mutant producing cell lines were isolated from five independent clones of 36-65. The mutant antibodies bind Ag 20 to more than 200-fold better than the parent …
Intravascular Metabolism Of Normal And Mutant Mouse Immunoglobulin Molecules, Roberta Pollock, Deborah French, Joshua Metlay, Barbara Birshtein, Matthew Scharff
Intravascular Metabolism Of Normal And Mutant Mouse Immunoglobulin Molecules, Roberta Pollock, Deborah French, Joshua Metlay, Barbara Birshtein, Matthew Scharff
Roberta Pollock
The metabolism of IgG immunoglobulins in the body is tightly regulated in order to maintain their intravascular concentration. Different subclasses may have different intravascular half-lives, and in the mouse, passively administered IgG2b disappears from the circulation more rapidly than IgG2a. We have attempted to localize the sequences in the constant region which are responsible for this difference by examining the intravascular metabolism of mutant immunoglobulins that were generated in tissue culture and have undergone deletions of individual constant region domains or contain different combinations of γ2b and γ2a CH2 and CH3 domains. Our results suggest that the regulation of intravascular …
Monoclonal Antibodies: A Powerful Tool For Selecting And Analyzing Mutations In Antigens And Antibodies, Roberta Pollock, Jean-Luc Teillaud, Matthew Scharff
Monoclonal Antibodies: A Powerful Tool For Selecting And Analyzing Mutations In Antigens And Antibodies, Roberta Pollock, Jean-Luc Teillaud, Matthew Scharff
Roberta Pollock
Antibodies have played an important role in microbiology. In addition to their uses in classifying microorganisms and studying evolutionary relationships, antibodies are useful for detecting the microheterogeneity of proteins that arises from recombination and somatic mutation. However, the usefulness of im munological assays has been complicated by the difficulty of repeatedly gener ating large amounts of antibodies with restricted reactivity, and the heter ogeneity of all antisera obtained by conventional methods. These problems reflect the fact that antisera contain antibodies secreted by many antibody producing B cell clones; hence such antibodies are termed polyclonal anti bodies. Even if the same …
Murine Cell Surface Immunoglobulin: Two Native Igd Structures., Roberta Pollock, Matthew Mescher
Murine Cell Surface Immunoglobulin: Two Native Igd Structures., Roberta Pollock, Matthew Mescher
Roberta Pollock
Murine spleen cell surface IgD is found in two forms upon SDS-polyacrylamide gel electrophoresis under nonreducing conditions. Both IgDI and IgDII are present on the surface of intact cells and appear to be native structures. Neither form could be accounted for by proteolytic degradation or disulfide bond rearrangement. IgDII has an apparent m.w. of 96,000, suggesting an HL structure. IgDI has an apparent m.w. of 150,000, approximately 33,000 daltons lower than that expected for an H2L2 structure. Significant variation in the relative amounts of IgDI and IgDII was found when mice of different strains were examined.