Medicine and Health Sciences Commons^™

In Vitro Exposure Of Jurkat T Cells To Industrially Important Organic Solvents In Binary Combination: Interaction Analysis, Catherine Mcdermott, Ashley Allshire, Frank Van Pelt, James Heffron

Catherine M. McDermott

Humans are frequently exposed to mixtures of environmental pollutants at low levels over prolonged periods of time yet most toxicity studies deal with acute exposure to high concentrations of single chemicals. Investigation of the biological effects and possible toxic interactions during long-term exposure to such mixtures is warranted. Here Jurkat T-cells were exposed to toluene, n-hexane and methyl ethyl ketone in binary combination. Concentration ranges were centered on thresholds at which the individual agents caused cell toxicity under otherwise similar conditions, and concentrations were confirmed by headspace gas chromatography. After 5 days cells were harvested and toxicity measured in terms …