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Full-Text Articles in Medicine and Health Sciences
Investigation Of Swine Dysentery Associated With "Brachyspira Hampsonii" Strain Eb107 And Comparison Of Diagnostic Methods, Bailey Lauren Wilberts
Investigation Of Swine Dysentery Associated With "Brachyspira Hampsonii" Strain Eb107 And Comparison Of Diagnostic Methods, Bailey Lauren Wilberts
Bailey (Wilberts) Arruda
Swine dysentery (SD) is an important cause of mucohemorrhagic diarrhea in pigs. Swine dysentery is associated with infection by Brachyspira hyodysenteriae which has historically been the only recognized strongly beta-hemolytic Brachyspira sp. However, in recent years, not all strongly beta-hemolytic isolates have been identified as B. hyodysenteriae using PCR assays specific for this species. Several reports have described putatively novel strongly beta-hemolytic Brachyspira spp. including "Brachyspira hampsonii" associated with SD. A pig inoculation study was used to compare lesions and colonic mucin expression associated with infection by B. hyodysenteriae or "B. hampsonii." Diagnosis of SD commonly …
Thoracoabdominal Myelolipomas And Carcinoma In A Lovebird (Agapomis Sp.), James R. Andreasen Jr., Claire B. Andreasen, Kenneth S. Latimer, Jeri L.H. Oliphant
Thoracoabdominal Myelolipomas And Carcinoma In A Lovebird (Agapomis Sp.), James R. Andreasen Jr., Claire B. Andreasen, Kenneth S. Latimer, Jeri L.H. Oliphant
Claire B. Andreasen
Myelolipomas are uncommon benign tumors composed of mature fat cells and hematopoietic cells (myeloid, erythroid, and lymphoid) in various proportions.1,4 In humans, these neoplasms rarely cause symptoms unless they are unusually large or unless they hemorrhage. Myelolipomas have been found incidentally at postmortem examination; however, their antemortem diagnosis is becoming more common in humans with increased use of ultrasound and computed tomography. In contrast, myelolipomas are reported infrequently in animals. Carcinomas (malignant tumors of epithelial origin) are found in many species of animals. In this report, we describe 2 intraabdominal myelolipomas and a cranial thoracic carcinoma of undetermined origin in …
Evaluation Of Chicken Heterophil Adherence, Claire B. Andreasen, Kenneth S. Latimer, W. L. Steffens
Evaluation Of Chicken Heterophil Adherence, Claire B. Andreasen, Kenneth S. Latimer, W. L. Steffens
Claire B. Andreasen
Adherence of chicken heterophils was evaluated at 37 C using preconstructed columns containing various weights of nylon fiber (75 mg, 100 mg, or 125 mg) and whole blood anticoagulated with sodium heparin or 10% disodium ethylenediamine tetraacetic acid (EDTA). Additionally, 50-mg and 75-mg nylon fiber columns incubated at 41 C were used to evaluate heterophil adherence at an increased temperature. The mean percent adherence for heparin-anticoagulated blood applied to 75-mg, 100-mg, and 125-mg nylon fiber columns at 37 C was 76%, 92% and 97.4%, respectively. Samples applied to 50-mg and 75-mg columns at 41 C had adherence values of 27% …
Separation Of Turkey Heterophils From Blood Using Two-Step Ficoll-Hypaque Discontinuous Gradients, Kenneth S. Latimer, Ingrid M. Kircher, Claire B. Andreasen
Separation Of Turkey Heterophils From Blood Using Two-Step Ficoll-Hypaque Discontinuous Gradients, Kenneth S. Latimer, Ingrid M. Kircher, Claire B. Andreasen
Claire B. Andreasen
A method is presented to separate turkey heterophils from anticoagulated whole blood using two-step Ficoll-Hypaque discontinuous gradients and ammonium chloride lysis of contaminating erythrocytes. Heterophils can be isolated from multiple blood samples within 3 to 4 hours. Using this technique, 66.4 +- 18.4% (mean +- standard deviation) of blood heterophils were harvested. Final cell isolates averaged 96.0 +- 2.9% heterophils with few contaminating eosinophils (2.5 +- 2.3%) or basophils (1.6 +- 1.8%). Cell viability, as determined by trypan blue dye exclusion, was 98.0 +- 1.4%.
Determination Of Chicken And Turkey Plasma And Serum Protein Concentrations By Refractometry And The Biuret Method, Claire B. Andreasen, Kenneth S. Latimer, Ingrid M. Kircher, John Brown
Determination Of Chicken And Turkey Plasma And Serum Protein Concentrations By Refractometry And The Biuret Method, Claire B. Andreasen, Kenneth S. Latimer, Ingrid M. Kircher, John Brown
Claire B. Andreasen
Plasma and serum protein concentrations were determined in chickens and turkeys by refractometry (with human and veterinary refractometers)and by the biuret method. Chicken and turkey serum protein values were significantly lower than respective plasma protein values according to both methods. Refractometer readings for both plasma and serum correlated closely with the results of the biuret test (r2 = 0.72 to 0.97). These findings indicate that plasma and serum protein values may be determined accurately in chickens and turkeys with a handheld refractometer.
Separation Of Avian Heterophils From Blood Using Ficoll-Hypaque Discontinuous Gradients, Claire B. Andreasen, Kenneth S. Latimer
Separation Of Avian Heterophils From Blood Using Ficoll-Hypaque Discontinuous Gradients, Claire B. Andreasen, Kenneth S. Latimer
Claire B. Andreasen
Rapid separation of avian heterophils from anticoagulated whole blood was achieved using Ficoll-Hypaque discontinuous gradients. An average of 14.4% of blood heterophils was harvested with a mean purity exceeding 99%. Heterophil viability, as determined by trypan blue dye exclusion, averaged 99.8%. The integrity of isolated heterophils was evaluated by cytochemical staining and ultrastructural examination. Cytochemical staining reactions of heterophils in whole blood and of isolated cell suspensions were similar. No ultrastructural abnormalities were observed. Using this procedure, viable intact heterophils were rapidly isolated from blood with an acceptable cell yield and purity for cell function studies.