Medicine and Health Sciences Commons^™

Cellular Proliferation Of Equine Bone Marrow- And Adipose Tissue-Derived Mesenchymal Stem Cells Decline With Increasing Donor Age, Jasmin Bagge, James N. Macleod, Lise C. Berg

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: Bone marrow (BM)- and adipose tissue (AT)-derived mesenchymal stem cells (MSCs) are used increasingly for autologous cell therapy in equine practice to treat musculoskeletal and other injuries. Current recommendations often call for 10–100 million MSCs per treatment, necessitating the expansion of primary cells in culture prior to therapeutic use. Of concern, human and rodent studies have shown a decline of both MSC recovery from sampled tissue and in vitro proliferative capacity with increasing donor age. This may be problematic for applications of autologous cell-based therapies in the important equine demographic of older patients.

Objectives: To investigate the effect of …

The Effect Of Hay Net Use On Cribbing Horses, Lauren Wesolowski

Undergraduate Honors Thesis Projects

Cribbing is a stereotypic behavior observed in horses, wherein the animal grasps a solid object with its front incisors and contracts its neck muscles to suck in air through its mouth, resulting in an audible grunt. Stereotypic behaviors such as cribbing are thought to be done in response to stress or insufficient stimulus in an animal’s living environment. Slow feeding hay nets can increase the amount of time horses spend eating daily hay rations. This research aims to use slow feeding hay nets to create a feeding process that is closer to natural grazing behavior and provide more stimulation for …

Commercial Genetic Testing For Type 2 Polysaccharide Storage Myopathy And Myofibrillar Myopathy Does Not Correspond To A Histopathological Diagnosis, Stephanie J. Valberg, C. J. Finno, Marisa L. Henry, Melissa Schott, Deborah Velez-Irizarry, Sichong Peng, Erica C. Mckenzie, Jessica L. Petersen

Department of Animal Science: Faculty Publications

Background: Commercial genetic tests for type 2 polysaccharide storage myopathy (PSSM2) and myofibrillar myopathy (MFM) have not been validated by peer-review, and formal regulation of veterinary genetic testing is lacking.

Objectives: To compare genotype and allele frequencies of commercial test variants (P variants) in MYOT (P2; rs1138656462), FLNC (P3a; rs1139799323), FLNC (P3b; rs1142918816) and MYOZ3 (P4; rs1142544043) between Warmblood (WB) and Arabian (AR) horses diagnosed with PSSM2/MFM by muscle histopathology, and phenotyped breed-matched controls. To quantify variant frequency in public repositories of ancient and modern horse breeds.

Study design: Cross sectional using archived clinical material and publicly available data.

Methods: …

Comparative Chondrogenesis Of Interzone And Anlagen Cells In Equine Skeletal Development, Chanhee Mok

Theses and Dissertations--Veterinary Science

At the presumptive sites of future synovial joints during mammalian skeletogenesis, articular cartilage develops from interzone located between the cartilaginous anlagen of bones. Thus, two types of cartilaginous tissues differentiate in close proximity. While anlagen cartilage is transient, progressing through endochondral ossification to form bones, articular cartilage is stable and functions throughout life to facilitate both low friction movement and load distribution. Despite important life-long functional properties, articular cartilage has a very limited intrinsic ability to repair structural defects. On the other hand, structural lesions in bones generally heal well by forming a cartilaginous callus and recapitulating endochondral ossification to …

Donor Age Effects On The Proliferative And Chondrogenic/Osteogenic Differentiation Performance Of Equine Bone Marrow- And Adipose Tissue Derived Mesenchymal Stem Cells In Culture, Jasmin Bagge

Theses and Dissertations--Veterinary Science

Orthopedic injuries are a major cause of lameness and morbidity in horses. Bone marrow (BM)- and adipose tissue (AT) derived mesenchymal stem cells (MSCs) have shown potential to facilitate the repair of orthopedic injuries and are being used increasingly in veterinary clinics. Presently, the use of MSCs as a therapy for equine patients is most commonly applied as autologous transplants, using BM- and AT-MSCs harvested from the patient shortly after the time of injury. Cell-based therapies are therefore delayed to enable primary cell numbers to be expanded in culture. Of concern, however, are human and rodent studies that have shown …

Impact Of The Individual Characteristics Of French Trotters On Their Racing Performance, Wanda Gorniak, Ewa Walkowicz, Maria Soroko, Mariusz Korczynski

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the influence of selected biometric characteristics on French trotters? racing performance. Moreover, the work attempts a prediction of racing performance indexes based on the horse?s individual characteristics, as well as the evaluation of the usefulness of an alternative method of a horse?s intravital measurements that limits the stress of the animal. A population of 64 horses of French trotter breed appearing in the Polish Stud Book of Trotters, which had participated in races in 2015, were subject to the investigation. The research material was represented by the indexes of racing performance combined …

Assessment Of Changes In The Saliva Cortisol Level Of Horses During Different Waysin Recreational Exploitation, Boguslawa Dlugosz, Tomasz Próchniak, Monika Stefaniuk-Szmukier, Marta Basiaga, Jaroslaw Luszczynski, Magdalena Pieszka

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the saliva cortisol levelsof recreational horses dependent on the type of horse activity. A total of 68 horses were included in the experiment and divided into groups according to recreational activity, type of horse, age, term of saliva collection, and sex. Saliva samples were obtained from each horse at 3 measurement points: in the morning (6 a.m.), in the evening (6 p.m.), and immediately after work. The overall analysis shows that the term of sample collection, recreational activity, and age influence cortisol concentration. The diurnal concentration of saliva cortisol does not differ …