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In Vitro Assay Development As An Alternative To The Use Of Laboratory Animals For Measuring Clostridium Perfringens Type C Toxoid, Lauren M. Wilmes
In Vitro Assay Development As An Alternative To The Use Of Laboratory Animals For Measuring Clostridium Perfringens Type C Toxoid, Lauren M. Wilmes
School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research
Clostridium perfringens type C produces beta toxin, which is a primary virulence determinate that can cause necrotic enteritis in neonatal animals. Vaccines directed against C. perfringens toxins have shown to be efficacious in preventing disease. However, the production of a commercial vaccine requires not only an efficacious antigen, but also testing methods for quantifying antigens and potency on final product. This research was undertaken to fulfill a need for the reduction in small animal usage to produce and deliver vaccines. The current testing methods are performed in vivo according to the Code of Federal Regulations number 9 (9CFR) United States …