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Full-Text Articles in Medicine and Health Sciences
Cloning And Sequence Analysis Of Orf5 Gene Of Porcine Reproductive And Respiratory Syndrome Virus Of The Zj Strain, Baocheng Hao, Dengyuan Niu, Xiaoyong Xing, Haitao Xiang, Fengqin Wen, Xiaoping Fu, Jianping Liang, Yonghao Hu
Cloning And Sequence Analysis Of Orf5 Gene Of Porcine Reproductive And Respiratory Syndrome Virus Of The Zj Strain, Baocheng Hao, Dengyuan Niu, Xiaoyong Xing, Haitao Xiang, Fengqin Wen, Xiaoping Fu, Jianping Liang, Yonghao Hu
The Thai Journal of Veterinary Medicine
A pair of the special primers was designed and synthesized according to the ORF5 sequence of porcine reproductive and respiratory syndrome virus (PRRSV), which was published in GenBank. In 2009 a pig farm in Zhejiang province suspected some diseased and dead pigs displaying symptoms of porcine reproductive and respiratory syndrome and provided 12 samples (spleen, kidney or lymph nodes) for laboratory diagnosis. The samples were positive of porcine reproductive and respiratory syndrome by PCR. The ORF5 gene was amplified by RT-PCR and cloned into pMD-18T vector. Nucleotide sequence was determined by sequencing and compared with other ORF5 genes in GenBank. …
Antiviral Activity Of Four Commercial Tilmicosin Preparations Against Porcine Reproductive And Respiratory Syndrome Virus (Prrsv): An In Vitro Study, Korakrit Poonsuk, Jirapat Arunorat, Yonlayong Woonwong, Panchan Sitthicharoenchai, Suphattra Jittimanee, Porjit Choojai, Roongroje Thanawongnuwech
Antiviral Activity Of Four Commercial Tilmicosin Preparations Against Porcine Reproductive And Respiratory Syndrome Virus (Prrsv): An In Vitro Study, Korakrit Poonsuk, Jirapat Arunorat, Yonlayong Woonwong, Panchan Sitthicharoenchai, Suphattra Jittimanee, Porjit Choojai, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
The efficacy of four commercial tilmicosin preparations (A, B, C and D) against type 2 porcine reproductive and respiratory syndrome virus (PRRSV), 01NP1 (Thai isolate), infection in cultured pulmonary alveolar macrophages (PAMs) was conducted in vitro. Primary PAMs were collected from four 4-week-old PRRSV-free pigs. After separately plated onto four 24-well plates, PAMs were treated separately by 4 commercial tilmicosin preparations of 2 concentrations each (0.1 mg/ml and 0.01 mg/ml). The treated PAMs were inoculated with 0.05 MOI of 01NP1 strain and were stained with PRRSV specific antibody using immunoperoxidase monolayer assay (IPMA) to evaluate the quantity of PRRSV infected …
Molecular Characterization And Expression Analysis Of Mir-29a In Porcine Cells And Porcine Reproductive And Respiratory Syndrome Virus Infected Peripheral Blood Mononuclear Cells, Win Surachetpong, Sunaree Nantakhruea, Porntippa Lekcharoensuk
Molecular Characterization And Expression Analysis Of Mir-29a In Porcine Cells And Porcine Reproductive And Respiratory Syndrome Virus Infected Peripheral Blood Mononuclear Cells, Win Surachetpong, Sunaree Nantakhruea, Porntippa Lekcharoensuk
The Thai Journal of Veterinary Medicine
MicroRNAs are a group of small non-coding RNA that are involved in posttranscriptional regulation through target mRNA degradation or inhibition of protein synthesis. The objective of this present study was to characterize and determine expression level of porcine miR-29a in various tissues. Our results indicated that porcine miR-29a shared high conserved sequence with miR-29a from other organisms. Furthermore, in silico analysis suggested that the seed region of porcine miR-29a partially matched IFN-γ mRNA. RT-PCR analysis indicated that miR-29a expressed in various tissues including lymph node, spleen, peripheral blood mononuclear cells (PBMCs) and PK-15 cells. Expression profiling of miR-29a in PRRSV-infected …