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Full-Text Articles in Medicine and Health Sciences
The Detection Of Ifn-Γ Positive Cells In The Lymphoid Tissues Of Prrsv-Infected Pigs Using Different Strains, For Infection, Tuddow Thaiwong, Chanis Leetheraprasert, Chaiwat Winyoonunthakul, Anudep Rungsipipat, Supradit Wangnaitham, Roongroje Thanawongnuwech
The Detection Of Ifn-Γ Positive Cells In The Lymphoid Tissues Of Prrsv-Infected Pigs Using Different Strains, For Infection, Tuddow Thaiwong, Chanis Leetheraprasert, Chaiwat Winyoonunthakul, Anudep Rungsipipat, Supradit Wangnaitham, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Archived paraffin-embedded lymphoid tissue samples from seventy five experimental PRRSV-infected pigs were examined for PRRSV antigen and IFN-γ positive cells, using immunohistochemistry. Lymphoid tissues (5 pigs/group) including spleen, tracheobronchial lymph nodes and tonsil came from negative control pigs (group 1), low virulence strain of PRRSV (RespPRRS/ReproTM, modified-live virus vaccine) infected pigs (group 2) and high virulence strain of PRRSV (VR-2385) infected pigs(group 3). They were necropsied at 3, 7,10, 14 or 28 days post-inoculation (DPI). According to the study, microscopic lesions in the lymphoid tissues from pigs infected with the high virulence strain had more severe lesions, characterized by severe …
Antigen Distribution Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In Thai Crossbred Pigs Using Immunohistochemistry, Porntip Laohasittikul, Nuttapon Boonarpha, Yongyut Pongprapachuen, Sawang Kesdangsakonwut, Supradit Wangnaitham, Roongroje Thanawongnuwech
Antigen Distribution Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In Thai Crossbred Pigs Using Immunohistochemistry, Porntip Laohasittikul, Nuttapon Boonarpha, Yongyut Pongprapachuen, Sawang Kesdangsakonwut, Supradit Wangnaitham, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Twenty, 3-week-old, crossbred pigs from a herd free from porcine reproductive and respiratory syndrome virus (PRRSV) were inoculated intranasally and intramusculary (1 ml each 102.5 TCID50/ml) with Thai PRRSV isolates of the US genotype (01NP1) (n=9) or the EU genotype (02SB3) (n=8). Three pigs were used as negative controls. Three infected and one control group pig were euthanatized 5, 9 or 15 days postinoculation (DPI) to detect and to compare the location and the amount of PRRSV-antigen present. Immunohistochemical evaluation of formalin-fixed tissues revealed PRRSV-antigen in the pulmonary macrophages of the lungs (6/9, 8/8 and 0/3 of the 01NP1-infected, 02SB3-infected …
Semiquantitative Analysis Of Il-10 Production In Bronchoalveolar Lavage Leukocytes Of Prrsv Infected Pigs, Permsak Thanawang, Chanida Yamkleebbua, Kritkong Shiyun, Roongroje Thanawongnuwech, Sawang Kesdangsakonwut, Supradit Wangnaitham, Sanipa Suradhat
Semiquantitative Analysis Of Il-10 Production In Bronchoalveolar Lavage Leukocytes Of Prrsv Infected Pigs, Permsak Thanawang, Chanida Yamkleebbua, Kritkong Shiyun, Roongroje Thanawongnuwech, Sawang Kesdangsakonwut, Supradit Wangnaitham, Sanipa Suradhat
The Thai Journal of Veterinary Medicine
Semi-quantitative analysis of the interleukin 10 (IL-10) in bronchoalveolar lavage cells (BALC), from pigs challenged with porcine reproductive and respiratory syndrome virus (PRRSV), was undertaken in this study. Twenty-one, three weeks old, crossbred pigs from a PRRSV-free commercial farm were separated into three groups, a control, non-infected group (3 pigs/group), and 2 groups (9 pigs/group), the first inoculated with a Thai-isolated European (O2SB3) strain and the second a Thai-isolated, North American (O1NP1) PRRSV strain. BALC were isolated from three infected pigs and one control on days 5, 9 and 15 post infection (DPI). The levels of IL-10 production in BALC …