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Full-Text Articles in Medicine and Health Sciences
Edwardsiella Ictaluri Infection In Rainbow Trout (Oncorhynchus Mykiss), Oktay Keski̇n, Selçuk Seçer, Müjgan İzgür, Süheyla Türkyilmaz, Rajhab Sawasawa Mkakosya
Edwardsiella Ictaluri Infection In Rainbow Trout (Oncorhynchus Mykiss), Oktay Keski̇n, Selçuk Seçer, Müjgan İzgür, Süheyla Türkyilmaz, Rajhab Sawasawa Mkakosya
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to isolate Edwardsiella ictaluri from diseased rainbow trout obtained from a commercial farm in the Ankara region, Turkey. Growth of the microorganism after 36 h of incubation was higher and purer at 28 ºC than at 37 ºC on all the organs (liver, spleen, kidney) of the 10 fish examined. Three groups, each consisting of 3 fish were used for experimental infection. All the fish in Group I, which received a pure culture suspension, died after treatment. There were no clinical symptoms of the disease or deaths in Group II treated with 1/50 concentration …
Inhibition Effect Of Some Chemical Anesthetics And Hypothermia On The Activity Of Glucose 6-Phosphate Dehydrogenase From Rainbow Trout (Oncorhynchus Mykiss) Erythrocytes In Vivo, Abdulkadi̇r Çi̇ltaş, Orhan Erdoğan, Olcay Hi̇sar, E. Mahmut Kocaman
Inhibition Effect Of Some Chemical Anesthetics And Hypothermia On The Activity Of Glucose 6-Phosphate Dehydrogenase From Rainbow Trout (Oncorhynchus Mykiss) Erythrocytes In Vivo, Abdulkadi̇r Çi̇ltaş, Orhan Erdoğan, Olcay Hi̇sar, E. Mahmut Kocaman
Turkish Journal of Veterinary & Animal Sciences
Many chemical anesthetics and drugs have been used in fish stripping, surgery and transportation, but their undesirable side effects on the body metabolism during treatment are not usually taken into consideration. In this study, we investigated the inhibition effect of some chemical anesthetics, such as Tranquil and tricaine methanesulfonate (MS-222), and hypothermia on glucose 6-phosphate dehydrogenase (G6PDH) activity. The control, initial, 1 h and 4 h values were 41.334 ± 3.190 EU (g Hb)^{-1}, 34.586 ± 5.086 EU (g Hb)^{-1}, 40.768 ± 3.430 EU (g Hb)^{-1} and 41.957 ± 3.157 EU (g Hb)^{-1}, respectively, in hypothermia. These values were 39.470 …
Inhibition Effects Of Some Antibiotics On The Activity Of Glucose 6-Phosphate Dehydrogenase Enzyme From Rainbow Trout (Oncorhynchus Mykiss Walbaum, 1792) Erythrocytes, Orhan Erdoğan, Mehmet Çi̇ftçi̇, Abdulkadi̇r Çi̇ltaş, Olcay Hi̇sar
Inhibition Effects Of Some Antibiotics On The Activity Of Glucose 6-Phosphate Dehydrogenase Enzyme From Rainbow Trout (Oncorhynchus Mykiss Walbaum, 1792) Erythrocytes, Orhan Erdoğan, Mehmet Çi̇ftçi̇, Abdulkadi̇r Çi̇ltaş, Olcay Hi̇sar
Turkish Journal of Veterinary & Animal Sciences
Inhibitory effects of some antibiotics on glucose 6-phosphate dehydrogenase from the erythrocytes of rainbow trout (Oncorhynchus mykiss Walbaum, 1792) were investigated. For this purpose, initially erythrocyte glucose 6-phosphate dehydrogenase was purified 1271.19-fold in a yield of 70.40% by using ammonium sulfate precipitation and 2',5'-ADP Sepharose 4B affinity gel. A constant temperature (+4 ºC) was maintained during the purification process. Enzyme activity was determined with the Beutler method by using a spectrophotometer at 340 nm. This method was utilized for all kinetic studies. Thiamphenicol, amikacin, gentamicin, and netilmicin were used as antibiotics. All the antibiotics exhibited inhibitory effects on the enzyme. …