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Full-Text Articles in Medicine and Health Sciences
The Mare Reproductive Loss Syndrome And The Eastern Tent Caterpillar Ii: A Toxicokinetic/Clinical Evaluation And A Proposed Pathogenesis: Septic Penetrating Setae, Thomas Tobin, J. Daniel Harkins, John Ford Roberts, Patricia W. Vanmeter, Tara A. Fuller
The Mare Reproductive Loss Syndrome And The Eastern Tent Caterpillar Ii: A Toxicokinetic/Clinical Evaluation And A Proposed Pathogenesis: Septic Penetrating Setae, Thomas Tobin, J. Daniel Harkins, John Ford Roberts, Patricia W. Vanmeter, Tara A. Fuller
Maxwell H. Gluck Equine Research Center Faculty Publications
Reviewing the mare reproductive loss syndrome (MRLS), it is proposed that the fundamental mechanism of this syndrome, which includes early fetal loss, late fetal loss, uveitis, pericarditis, and encephalitis, is tissue penetration by septic barbed setal fragments (septic penetrating setae) from Eastern tent caterpillars (Malacosoma americanum). Once ingested, these barbed setal fragments migrate through moving tissues, followed by rapid hematogenous spread of bacteria, bacterial emboli, and/or septic fragments of setae (septic penetrating setal emboli), collectively referred to as septic materials. Pathogenic bacteria, therefore, enter the horse as hitchhikers on or in the caterpillar setal fragments, and MRLS is caused by …
A Gc-Ms Method For The Determination Of Isoxsuprine In Biological Fluids Of The Horse Utilizing Electron Impact Ionization, Jeffrey M Bosken, A. F. Lehner, C. G. Hughes, W. E. Woods, F. C. Camargo, J. D. Harkins, J. Boyles, Thomas Tobin
A Gc-Ms Method For The Determination Of Isoxsuprine In Biological Fluids Of The Horse Utilizing Electron Impact Ionization, Jeffrey M Bosken, A. F. Lehner, C. G. Hughes, W. E. Woods, F. C. Camargo, J. D. Harkins, J. Boyles, Thomas Tobin
Maxwell H. Gluck Equine Research Center Faculty Publications
Isoxsuprine is used to treat navicular disease and other lower-limb problems in the horse. Isoxsuprine is regulated as a class 4 compound by the Association of Racing Commissioners, International (ARCI) and, thus, requires regulatory monitoring. A gas chromatography-mass spectrometry method utilizing electron impact ionization was developed and validated for the quantitation of isoxsuprine in equine plasma or equine urine. The method utilized robotic solid-phase extraction and tri-methyl silyl ether products of derivatization. Products were bis-trimethylsilyl (TMS) isoxsuprine and tris-TMS ritodrine, which released intense quantifier ions m/z 178 for isoxsuprine and m/z 236 for ritodrine that were products of C-C cleavage. …
Detection And Confirmation Of Ractopamine And Its Metabolites In Horse Urine After Paylean® Administration, A. F. Lehner, C. G. Hughes, J Daniel Harkins, C. Nickerson, B. Mollett, L. Dirikolu, Jeffrey M Bosken, Fernanda C. Camargo, J. Boyles, A. Troppmann, W. Karpiesiuk, W. E. Woods, Thomas Tobin
Detection And Confirmation Of Ractopamine And Its Metabolites In Horse Urine After Paylean® Administration, A. F. Lehner, C. G. Hughes, J Daniel Harkins, C. Nickerson, B. Mollett, L. Dirikolu, Jeffrey M Bosken, Fernanda C. Camargo, J. Boyles, A. Troppmann, W. Karpiesiuk, W. E. Woods, Thomas Tobin
Maxwell H. Gluck Equine Research Center Faculty Publications
We have investigated the detection, confirmation, and metabolism of the beta-adrenergic agonist ractopamine administered as Paylean to the horse. A Testing Components Corporation enzyme-linked imunosorbent assay (ELISA) kit for ractopamine displayed linear response between 1.0 and 100 ng/ml, with an 1-50 of 10 ng/ml, and an effective screening limit of detection of 50 ng/mL. The kit was readily able to detect ractopamine equivalents in unhydrolyzed urine up to 24 h following a 300-mg oral dose. Gas chromatography-mass spectrometry (GC-MS) confirmation comprised glucuronidase treatment, solid-phase extraction, and trimethylsilyl derivatization, with selected-ion monitoring of ractopamine-tris(trimethylsilane) (TMS) m/z 267, 250, 179, and 502 …
Development Of A Method For The Detection And Confirmation Of The Alpha-2 Agonist Amitraz And Its Major Metabolite In Horse Urine, A. F. Lehner, C. G. Hughes, W. Karpiesiuk, J Daniel Harkins, L. Dirikolu, Jeffrey M Bosken, Fernanda Camargo, J. Boyles, A. Troppmann, W. E. Woods, Thomas Tobin
Development Of A Method For The Detection And Confirmation Of The Alpha-2 Agonist Amitraz And Its Major Metabolite In Horse Urine, A. F. Lehner, C. G. Hughes, W. Karpiesiuk, J Daniel Harkins, L. Dirikolu, Jeffrey M Bosken, Fernanda Camargo, J. Boyles, A. Troppmann, W. E. Woods, Thomas Tobin
Maxwell H. Gluck Equine Research Center Faculty Publications
Amitraz (N′-(2,4-dimethylphenyl)-N-[[(2,4-dimethylphenyl)imino] methyl]-N-methyl-methanimidamide) is an alpha-2 adrenergic agonist used in veterinary medicine primarily as a scabicide- or acaricide-type insecticide. As an alpha-2 adrenergic agonist, it also has sedative/tranquilizing properties and is, therefore, listed as an Association of Racing Commissioners International Class 3 Foreign Substance, indicating its potential to influence the outcome of horse races. We identified the principal equine metabolite of amitraz as N-2,4-dimethylphenyl-N′-methylformamidine by electrospray ionization(+)-mass spectrometry and developed a gas chromatographic-mass spectrometric (GC-MS) method for its detection, quantitation, and confirmation in performance horse regulation. The GC-MS method involves derivatization with t-butyldimethylsilyl groups; selected ion monitoring (SIM) of m/z …