Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Medicine and Health Sciences
Sds-Page Analysis For Protein Patterns Of Adults Haemonchus Placei, Mecistocirrus Digitalis And Cooperia Spp. In Cattle, Sonthaya Tiawsirisup, Amonthep Khuprathumsiri, Suwannee Nithiuthai, Nikom Chaisiri, Thirayuth Kositamongkol, Sudchit Chungpivat
Sds-Page Analysis For Protein Patterns Of Adults Haemonchus Placei, Mecistocirrus Digitalis And Cooperia Spp. In Cattle, Sonthaya Tiawsirisup, Amonthep Khuprathumsiri, Suwannee Nithiuthai, Nikom Chaisiri, Thirayuth Kositamongkol, Sudchit Chungpivat
The Thai Journal of Veterinary Medicine
Protein profiles of crude extracts from adult male and female Haemonchus placei, Mecistocirrus digitatus and Cooperia spp. were studied. The parasites were collected from the abomasum and duodenum of cattle at Pathumthani and Nonthaburi’s abattoirs. SDS-PAGE analysis of the crude extracts revealed, protein bands of various intensities ranging from 13- 24 bands with a molecular mass of between 19-130 kDa. The protein band patterns of each parasite at a molecular weight of between 48.2-111 kDa were similar whereas other protein bands at a molecular mass below 48.2 kDa were different. The number of distinct bands shown by the crude extract …
Analysis Of Fluids Of Hydatid Cysts From Sheep By Sds-Page, And Determination Of Specific Antigens In Protein Structure By Western Blotting, Ayşe Burgu, Ahmet Doğanay, Bahadir Gönenç, H. Oğuz Sarimehmetoğlu, Funda Kalinbacak
Analysis Of Fluids Of Hydatid Cysts From Sheep By Sds-Page, And Determination Of Specific Antigens In Protein Structure By Western Blotting, Ayşe Burgu, Ahmet Doğanay, Bahadir Gönenç, H. Oğuz Sarimehmetoğlu, Funda Kalinbacak
Turkish Journal of Veterinary & Animal Sciences
Protein bands of hydatid fluids of sheep liver were determined by SDS-PAGE and Western blotting, and this was followed by the determination of specific bands of hydatid cysts for sheep and humans. For diagnostic purposes, 30 positive and 20 negative sheep sera, 1 commercially obtained non-infected sheep serum, and 10 posi- tive and 10 negative human sera were used in this experiment. According to our results, the specific protein band for hydatid dis- ease in sheep was determined as 116 kDa, while the specific protein bands for hydatid disease in humans were determined as 68 and 8 kDa. We determined …