Medicine and Health Sciences Commons^™

Enhanced Pathogenicity Of Candida Albicans Pre-Treated With Subinhibitory Concentrations Of Fluconazole In A Mouse Model Of Disseminated Candidiasis, Dhammika H.M.L.P. Navarathna, Jacob M. Hornby, Natasha Hoerrmann, Anne M. Parkhurst, Gerald E. Duhamel, Kenneth Nickerson

School of Veterinary and Biomedical Sciences: Faculty Publications

Objectives: To investigate the relative pathogenicity of Candida albicans treated with subinhibitory concentrations of fluconazole in a mouse model of disseminated candidiasis. Previous studies indicate that these cells secrete 10 times more farnesol than do untreated cells. In our usage, subinhibitory means a concentration which causes a prominent decrease in turbidity but still allows some cell growth. Methods: C. albicans A72 cells were grown overnight in 0–5.0 mM fluconazole, washed, and inoculated in mice by tail vein injection. Groups of 15 or 16 mice were injected with 1.3 • 10⁶cells and mortality was recorded for 7 days post-inoculation. …