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Evaluation Of A Semiquantitative Snap Test For Measurement Of Bile Acids In Dogs, Rachel L. Seibert, Karen M. Tobias, Ann Reed, Karl R. Snyder

Ann Reed

Background. Serum bile acids (SBA) are used as a routine screening tool of liver function in dogs. Serum samples are usually shipped to a referral laboratory for quantitative analysis with an enzymatic chemistry analyzer. The canine SNAP Bile Acids Test (SNAP-BAT) provides an immediate, semi-quantitative measurement of bile acid concentrations in-house. With the SNAP-BAT, bile acids concentrations of 5–30 µmol/L are quantified, and results outside of that range are classified as <5 or >30 µmol/L. Agreement of the SNAP-BAT with the enzymatic method has not been extensively investigated.

Objectives. The purposes of this prospective clinical study were to assess the precision of …

Evaluation Of A Semiquantitative Snap Test For Measurement Of Bile Acids In Dogs, Rachel L. Seibert, Karen M. Tobias, Ann Reed, Karl R. Snyder

Karen M. Tobias DVM, DACVS, College of Veterinary Medicine

Background. Serum bile acids (SBA) are used as a routine screening tool of liver function in dogs. Serum samples are usually shipped to a referral laboratory for quantitative analysis with an enzymatic chemistry analyzer. The canine SNAP Bile Acids Test (SNAP-BAT) provides an immediate, semi-quantitative measurement of bile acid concentrations in-house. With the SNAP-BAT, bile acids concentrations of 5–30 µmol/L are quantified, and results outside of that range are classified as <5 or >30 µmol/L. Agreement of the SNAP-BAT with the enzymatic method has not been extensively investigated. Objectives. The purposes of this prospective clinical study were to assess the precision of …

Protein C Activity In Dogs: Adaptation Of A Commercial Human Colorimetric Assay And Evaluation Of Effects Of Storage Time And Temperature, Michael M. Fry, Karl R. Snyder, Karen M. Tobias, Baye G. Williamson, Ann Reed

Michael M Fry

Objectives of this study were to adapt a commercial human protein C (PC) colorimetric assay for use in dogs and to investigate effects of various storage conditions. The human assay was modified by using pooled canine plasma for calibration and by increasing the activation time. PC activity was measured in fresh canine plasma and in plasma stored under various conditions. PC activity of some stored samples was significantly different from that of fresh plasma; however, differences were small. No difference was detected in samples stored under similar conditions but analyzed in different laboratories using similar methodology. Results of this study …