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Distribution Of Anti-Cd68 (Ebm11) Immunoreactivity In Formalin-Fixed, Paraffin-Embedded Bovine Tissues, Mark R. Ackermann, B. M. Debey, T. J. Stabel, J. H. Gold, K. B. Register, J. T. Meehan

Mark R. Ackermann

A commercially acquired anti-human macrophage antibody (anti-CD68; EBM11) was used in an immunocytochemical technique to detect macrophages in formalin-fixed, paraffin-embedded tissues from cattle, pigs, humans, rats, turkeys, dogs, and cats. In healthy cattle, the antibody labeled alveolar macrophages, pulmonary intravascular cells (presumably intravascular macrophages), and macrophage-like cells in other tissues. In bovine lungs infected with Pasteurella haemolytica, EBM11 antibody labeled 95% of alveolar macrophages and macrophages within alveolar septa but only 0–2% of streaming or “oat” leukocytes. Alveolar macrophages were also stained by EBM11 in pigs but not in rats, turkeys, dogs, and cats. The antibody also stained macrophage aggregates …