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Full-Text Articles in Medicine and Health Sciences
The Expression Of C-Erbb2 Oncogene Protein As A Prognostic Factor In Feline Mammary Tumors, Mataya Taweechart, On-Uma Sirayayon, Grisnarong Wongbandue, Supradit Wangnaitham, Achariya Sailasuta, Sudson Sirividhayapong, Anudep Rungsipipat
The Expression Of C-Erbb2 Oncogene Protein As A Prognostic Factor In Feline Mammary Tumors, Mataya Taweechart, On-Uma Sirayayon, Grisnarong Wongbandue, Supradit Wangnaitham, Achariya Sailasuta, Sudson Sirividhayapong, Anudep Rungsipipat
The Thai Journal of Veterinary Medicine
The study looked at the relationship between the expression of c-erbB2 oncogene protein, histological types, PCNA and AgNORs indices, in feline mammary tumors. Sixty, surgically resected, mammary tumors from cats were collected and histological processed. The expression of c-erbB2 oncogene protein was detected by immunohistochemistry and was seen in benign hyperplasia, adenosis and malignant groups. The c-erbB2 oncogene protein expression in malignant, feline mammary tumors was 41.67% (25/60) which could be divided into simple tubular ACC, 25% (6/24), simple papillary ACC, 78.6% (11/14), solid carcinoma, 26.7% (4/15) and cribiform carcinoma, 57.14% (4/7). There were statistically significantly differences (p<0.05) in the expression of c-erbB2 oncogene protein between simple tubular ACC, compared with simple papillary ACC and solid carcinomas. The expression of c-erbB2 oncogene protein in specimens, classified by histological malignancy grades revealed, grade I, 42.11% and grade II, 41.46% but there was no statistically significant difference between them. An evaluation of a proliferative marker shown by a mean (±SD) of PCNA, was 34.63±23.87 and the mean (±SD) of AgNORs was 1.23±0.18. There was no significant difference between the PCNA and AgNORs index of both the histological types and the histological malignancy grades. However, a statistical difference of mean PCNA and AgNORs indices was noted in both c-erbB2 positive and c-erbB2 negative groups. The mean PCNA indices and AgNORs indices in c-erbB2 positive samples were 26.52±18.68 and 1.12±0.27 respectively, while the c-erbB2 negative ones were 39.43±26.24 and 1.28±0.19 respectively. In conclusion, c-erbB2 oncogene protein expression is considered to be an effective prognostic factor for adenocarcinoma subtypes of feline mammary tumors as it is in human and canine cases. However, integration of all the parameters (c-erbB2 oncogene protein, PCNA index, AgNORs index, histological types and clinical data) may achieve a more precise prognosis in feline, mammary tumor cases.
Antigen Distribution Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In Thai Crossbred Pigs Using Immunohistochemistry, Porntip Laohasittikul, Nuttapon Boonarpha, Yongyut Pongprapachuen, Sawang Kesdangsakonwut, Supradit Wangnaitham, Roongroje Thanawongnuwech
Antigen Distribution Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In Thai Crossbred Pigs Using Immunohistochemistry, Porntip Laohasittikul, Nuttapon Boonarpha, Yongyut Pongprapachuen, Sawang Kesdangsakonwut, Supradit Wangnaitham, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Twenty, 3-week-old, crossbred pigs from a herd free from porcine reproductive and respiratory syndrome virus (PRRSV) were inoculated intranasally and intramusculary (1 ml each 102.5 TCID50/ml) with Thai PRRSV isolates of the US genotype (01NP1) (n=9) or the EU genotype (02SB3) (n=8). Three pigs were used as negative controls. Three infected and one control group pig were euthanatized 5, 9 or 15 days postinoculation (DPI) to detect and to compare the location and the amount of PRRSV-antigen present. Immunohistochemical evaluation of formalin-fixed tissues revealed PRRSV-antigen in the pulmonary macrophages of the lungs (6/9, 8/8 and 0/3 of the 01NP1-infected, 02SB3-infected …
Immunohistochemical Distribution Of Cells Containing Insulin, Glucagon And Somatostatin In The Goose (Anser Anser) Pancreas, Nurhayat Gülmez, Hakan Kocamiş, Şahi̇n Aslan, Mümtaz Nazli
Immunohistochemical Distribution Of Cells Containing Insulin, Glucagon And Somatostatin In The Goose (Anser Anser) Pancreas, Nurhayat Gülmez, Hakan Kocamiş, Şahi̇n Aslan, Mümtaz Nazli
Turkish Journal of Veterinary & Animal Sciences
The purpose of the present study was to determine the distribution of cells containing glucagon (A), insulin (B), and somatostatin (D) in the goose pancreas. Small pieces of tissue from 5 adult geese (Anser anser) pancreases were dissected under deep ether anaesthesia. Sections were stained with Crossmon's connective tissue stain for general observations and Gomori's method for pancreatic islet cells. Sections were further processed for standard immunohistochemical techniques using the avidin-biotin-complex method for the distribution of A, B and D cells. There were no B cells inside the A islets, which were mainly composed of A cells. B islets were …