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Full-Text Articles in Medicine and Health Sciences
A Comparison Between Three Serological Tests Forbrucella Melitensis Infection In Sheep, Nizar Abuharfeil, Mahmoud N. Abo-Shehada
A Comparison Between Three Serological Tests Forbrucella Melitensis Infection In Sheep, Nizar Abuharfeil, Mahmoud N. Abo-Shehada
Turkish Journal of Veterinary & Animal Sciences
A comparative study of the Rose Bengal test (RBT), Enzyme-Linked Immunosorbent Assay (ELISA) and complement fixation test (CFT) was performed to analyze sera of two Awassi flocks. Flock A was composed of 215 ewes, naturally infected with brucellosis. None of the animals were vaccinated. Flock B consisted of 336 non-infected non-vaccinated ewes. These served as nega-tive controls. The two flocks were each divided into 4 groups, and samples were collected from one group every 6 months and subjected to bacteriologic and serologic testing. Samples from any aborted ewes were also collected immediately after abortion and 6 weeks later. During the …
Demonstration Of Infectious Bovine Rhinotracheitis (Ibr) Virus Antigens Byimmunoperoxidase Staining, Hakan Bulut, Yusuf Bolat, Aykut Özdarendeli̇, Mehmet Z. Doymaz, S. İsmet Gürhan
Demonstration Of Infectious Bovine Rhinotracheitis (Ibr) Virus Antigens Byimmunoperoxidase Staining, Hakan Bulut, Yusuf Bolat, Aykut Özdarendeli̇, Mehmet Z. Doymaz, S. İsmet Gürhan
Turkish Journal of Veterinary & Animal Sciences
LUT, Yusuf BOLAT, Aykut ÖZDARENDELİ Fırat Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Elazığ-TÜRKİYE Mehmet Z. DOYMAZ Fırat Üniversitesi, Tip Fakültesi, Mikrobiyoloji Anabilim Dalı, Elazığ-TÜRKİYE S. İsmet GÜRHAN Şap Enstitüsü, Ankara-TÜRKİYE Abtract: In this study, cellular localization of IBR-IPV (Infectious Bovine Rhinotracheitis-Infectious Pustular Vulvovaginitis) virus anti-gens at various times of infection was studied. For this purpose, MDBK cells wre infected with IBR-Colorado strain and cellular dis-tribution of viral antigens were followed at early and late periods of infetction. Immunohistochemical staining of virus antigens were performed by using IBR-Colorado immune Balb/C mouse serum and horse radish peroxidase-conjugated goat anti-mouse immuno-globins as primary …
Detection Of Antibodies To Ehrlichia Risticii In Horses, Seyyal Ak, Nuri Turan, Ahmet Mi̇nbay, Sukanta K. Dutta
Detection Of Antibodies To Ehrlichia Risticii In Horses, Seyyal Ak, Nuri Turan, Ahmet Mi̇nbay, Sukanta K. Dutta
Turkish Journal of Veterinary & Animal Sciences
In this study, presence of antibodies to Ehrlichia risticii in the sera of 100 throughbreed horses brought to Turkey Jockey Club Stud from different regions was investigated by indirect fluorescent antibody (IFA), western immunoblotting, indirect enzyme linked immunosorbent assay (ELISA) and competetive ELISA (CELISA) using monoclonal antibody. Ten sera gave various degrees of flourescence on IFA test. The sera giving fluorescence were sent to University of Maryland and the test was repeated. The results were similar. Five sera from those positives were also tested on western immunoblotting. Amongst those, only one serum reacted with one of the 9 surface antigens …