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Full-Text Articles in Medicine and Health Sciences
Dynamics And Evolution Of Genotype 1 Porcine Reproductive And Respiratory Syndrome Virus Following Its Introduction Into A Herd Concurrently Infected With Genotypes 1 And 2, Thitima Tripipat, Kepalee Saeng-Chuto, Adthakorn Madapong, Christopher James Stott, Angkana Tantituvanont, Pavita Kaewprommal, Jittima Piriyapongsa, Dachrit Nilubol
Dynamics And Evolution Of Genotype 1 Porcine Reproductive And Respiratory Syndrome Virus Following Its Introduction Into A Herd Concurrently Infected With Genotypes 1 And 2, Thitima Tripipat, Kepalee Saeng-Chuto, Adthakorn Madapong, Christopher James Stott, Angkana Tantituvanont, Pavita Kaewprommal, Jittima Piriyapongsa, Dachrit Nilubol
The Thai Journal of Veterinary Medicine
This study was conducted to investigate the dynamics and evolution of porcine reproductive and respiratory syndrome virus (PRRSV) genotype 1 following its introduction into a herd concurrently infected with both PRRSV genotypes. The results demonstrated that 2 novel genotype 1 groups emerged, termed clusters II and III. The emergence of cluster II suggested that this cluster of genotype 1 virus potentially correlated with the outbreak. Then, cluster III was detected with the disappearance of cluster II. The evolution of the genotype 1 virus from cluster II to cluster III is likely to be associated with immune evasion induced by amino …
Effect Of Oxidized Soybean Oil On The Immune Response To Porcine Reproductive And Respiratory Syndrome Modified Live Virus Vaccine In Nursery Pigs, Alongkot Boonsoongnern, Pichai Jirawattanapong, Win Surachetpong, Prapassorn Boonsoongnern, Pariwat Poolperm
Effect Of Oxidized Soybean Oil On The Immune Response To Porcine Reproductive And Respiratory Syndrome Modified Live Virus Vaccine In Nursery Pigs, Alongkot Boonsoongnern, Pichai Jirawattanapong, Win Surachetpong, Prapassorn Boonsoongnern, Pariwat Poolperm
The Thai Journal of Veterinary Medicine
This study evaluated the effect of oxidized soybean oil on the immune response to porcine reproductive and respiratory syndrome modified live vaccine (PRRS-MLV), and the oxidative stress status in nursery pigs.Seventycastrated weaned pigs, from a free PRRS virus infected herd, were divided into two groups: treatment group (n=63) and control group (n=7). The treatment pigs were vaccinated with the PRRS-MLV and then divided into three groups based on different diet types containing different soybean oils: (A) 5% fresh oil, (B) 5% heated oil for 43 h, and (C) 5% heated oil for 38 h. The control pigs were not vaccinated …
Detection Of Prrsv Circulation Using Oral Fluid Samples For Nursery Management In Endemically Prrsv-Infected Farms, Yonlayong Woonwong, Roongtham Kedkovid, Jirapat Arunorat, Chaitawat Sirisereewan, Teerawut Nedumpun, Korakrit Poonsuk, Yaowalak Panyasing, Pariwat Poolperm, Alongkot Boonsoongnern, Roongroje Thanawongnuwech
Detection Of Prrsv Circulation Using Oral Fluid Samples For Nursery Management In Endemically Prrsv-Infected Farms, Yonlayong Woonwong, Roongtham Kedkovid, Jirapat Arunorat, Chaitawat Sirisereewan, Teerawut Nedumpun, Korakrit Poonsuk, Yaowalak Panyasing, Pariwat Poolperm, Alongkot Boonsoongnern, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Porcine reproductive and respiratory syndrome (PRRS) has become a major swine disease worldwide. Relevant management strategies and diagnostic assays are of importance for PRRS virus (PRRSV) control. The objective of this study was to determine the use of oral fluids for PRRSV monitoring in endemically PRRSV-infected herds. PRRSV RNA and PRRSV-specific antibodies were monitored using oral fluid samples and serum samples in two conventional swine farms in Thailand (‘Farm A’, a one-site conventional system farm, and ‘Farm B’, a one-site P0-P1 segregation system farm) during farrowing to nursery periods. Both PRRSV RNA and PRRSV antibodies were detected from 3 to …
Expression Of Mir-29a And Ifn-Γ In Pigs Vaccinated With Modified Live Prrs Vaccine, Sunaree Nantakhruea, Nuntawan Aunjangwang, Orawan Limsivilai, Win Surachetpong
Expression Of Mir-29a And Ifn-Γ In Pigs Vaccinated With Modified Live Prrs Vaccine, Sunaree Nantakhruea, Nuntawan Aunjangwang, Orawan Limsivilai, Win Surachetpong
The Thai Journal of Veterinary Medicine
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the important RNA viruses that affects swine production worldwide. To better improve vaccine efficacy, understanding the molecular mechanisms and viral-host interaction is important to increase the knowledge of vaccine response. The objective of this study was to investigate the expression levels of miR-29a and IFN-γ in pigs that received modified live PRRSV vaccine. The study revealed that both miR-29a and IFN-γ transcript levels increased in vivo early, at day 3 post vaccination, whereas levels of miR-29a and IFN-γ mRNA were not different at day 7 post vaccination. Our study is …
Antiviral Activity Of Thymus Vulgaris And Nepeta Cataria Hydrosols Against Porcine Reproductive And Respiratory Syndrome Virus, Kraijak Kaewprom, Yu-Hsin Chen, Chuen-Fu Lin, Ming-Tang Chiou, Chao-Nan Lin
Antiviral Activity Of Thymus Vulgaris And Nepeta Cataria Hydrosols Against Porcine Reproductive And Respiratory Syndrome Virus, Kraijak Kaewprom, Yu-Hsin Chen, Chuen-Fu Lin, Ming-Tang Chiou, Chao-Nan Lin
The Thai Journal of Veterinary Medicine
Porcine reproductive and respiratory syndrome (PRRS) is characterized by reproductive failure of sows and respiratory problems of nursery and growing pigs. Current management strategies mainly focus on preventing PRRS virus (PRRSV) infection through vaccination. However, the currently available modified live vaccines are not sufficient to eradicate the virus and do not provide complete immunity from infection. In search of agents that may be proven clinically effective against North American PRRSV (NA-PRRSV or US-PRRSV) infection, 42 natural herb hydrosols were purified and subjected to screening of their antiviral activity against two US-PRRSV strains in MARC-145 cells. Based on results obtained from …
Cloning And Sequence Analysis Of Orf5 Gene Of Porcine Reproductive And Respiratory Syndrome Virus Of The Zj Strain, Baocheng Hao, Dengyuan Niu, Xiaoyong Xing, Haitao Xiang, Fengqin Wen, Xiaoping Fu, Jianping Liang, Yonghao Hu
Cloning And Sequence Analysis Of Orf5 Gene Of Porcine Reproductive And Respiratory Syndrome Virus Of The Zj Strain, Baocheng Hao, Dengyuan Niu, Xiaoyong Xing, Haitao Xiang, Fengqin Wen, Xiaoping Fu, Jianping Liang, Yonghao Hu
The Thai Journal of Veterinary Medicine
A pair of the special primers was designed and synthesized according to the ORF5 sequence of porcine reproductive and respiratory syndrome virus (PRRSV), which was published in GenBank. In 2009 a pig farm in Zhejiang province suspected some diseased and dead pigs displaying symptoms of porcine reproductive and respiratory syndrome and provided 12 samples (spleen, kidney or lymph nodes) for laboratory diagnosis. The samples were positive of porcine reproductive and respiratory syndrome by PCR. The ORF5 gene was amplified by RT-PCR and cloned into pMD-18T vector. Nucleotide sequence was determined by sequencing and compared with other ORF5 genes in GenBank. …
Antiviral Activity Of Four Commercial Tilmicosin Preparations Against Porcine Reproductive And Respiratory Syndrome Virus (Prrsv): An In Vitro Study, Korakrit Poonsuk, Jirapat Arunorat, Yonlayong Woonwong, Panchan Sitthicharoenchai, Suphattra Jittimanee, Porjit Choojai, Roongroje Thanawongnuwech
Antiviral Activity Of Four Commercial Tilmicosin Preparations Against Porcine Reproductive And Respiratory Syndrome Virus (Prrsv): An In Vitro Study, Korakrit Poonsuk, Jirapat Arunorat, Yonlayong Woonwong, Panchan Sitthicharoenchai, Suphattra Jittimanee, Porjit Choojai, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
The efficacy of four commercial tilmicosin preparations (A, B, C and D) against type 2 porcine reproductive and respiratory syndrome virus (PRRSV), 01NP1 (Thai isolate), infection in cultured pulmonary alveolar macrophages (PAMs) was conducted in vitro. Primary PAMs were collected from four 4-week-old PRRSV-free pigs. After separately plated onto four 24-well plates, PAMs were treated separately by 4 commercial tilmicosin preparations of 2 concentrations each (0.1 mg/ml and 0.01 mg/ml). The treated PAMs were inoculated with 0.05 MOI of 01NP1 strain and were stained with PRRSV specific antibody using immunoperoxidase monolayer assay (IPMA) to evaluate the quantity of PRRSV infected …
Molecular Characterization And Expression Analysis Of Mir-29a In Porcine Cells And Porcine Reproductive And Respiratory Syndrome Virus Infected Peripheral Blood Mononuclear Cells, Win Surachetpong, Sunaree Nantakhruea, Porntippa Lekcharoensuk
Molecular Characterization And Expression Analysis Of Mir-29a In Porcine Cells And Porcine Reproductive And Respiratory Syndrome Virus Infected Peripheral Blood Mononuclear Cells, Win Surachetpong, Sunaree Nantakhruea, Porntippa Lekcharoensuk
The Thai Journal of Veterinary Medicine
MicroRNAs are a group of small non-coding RNA that are involved in posttranscriptional regulation through target mRNA degradation or inhibition of protein synthesis. The objective of this present study was to characterize and determine expression level of porcine miR-29a in various tissues. Our results indicated that porcine miR-29a shared high conserved sequence with miR-29a from other organisms. Furthermore, in silico analysis suggested that the seed region of porcine miR-29a partially matched IFN-γ mRNA. RT-PCR analysis indicated that miR-29a expressed in various tissues including lymph node, spleen, peripheral blood mononuclear cells (PBMCs) and PK-15 cells. Expression profiling of miR-29a in PRRSV-infected …
The Detection Of Ifn-Γ Positive Cells In The Lymphoid Tissues Of Prrsv-Infected Pigs Using Different Strains, For Infection, Tuddow Thaiwong, Chanis Leetheraprasert, Chaiwat Winyoonunthakul, Anudep Rungsipipat, Supradit Wangnaitham, Roongroje Thanawongnuwech
The Detection Of Ifn-Γ Positive Cells In The Lymphoid Tissues Of Prrsv-Infected Pigs Using Different Strains, For Infection, Tuddow Thaiwong, Chanis Leetheraprasert, Chaiwat Winyoonunthakul, Anudep Rungsipipat, Supradit Wangnaitham, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Archived paraffin-embedded lymphoid tissue samples from seventy five experimental PRRSV-infected pigs were examined for PRRSV antigen and IFN-γ positive cells, using immunohistochemistry. Lymphoid tissues (5 pigs/group) including spleen, tracheobronchial lymph nodes and tonsil came from negative control pigs (group 1), low virulence strain of PRRSV (RespPRRS/ReproTM, modified-live virus vaccine) infected pigs (group 2) and high virulence strain of PRRSV (VR-2385) infected pigs(group 3). They were necropsied at 3, 7,10, 14 or 28 days post-inoculation (DPI). According to the study, microscopic lesions in the lymphoid tissues from pigs infected with the high virulence strain had more severe lesions, characterized by severe …
Semiquantitative Analysis Of Il-10 Production In Bronchoalveolar Lavage Leukocytes Of Prrsv Infected Pigs, Permsak Thanawang, Chanida Yamkleebbua, Kritkong Shiyun, Roongroje Thanawongnuwech, Sawang Kesdangsakonwut, Supradit Wangnaitham, Sanipa Suradhat
Semiquantitative Analysis Of Il-10 Production In Bronchoalveolar Lavage Leukocytes Of Prrsv Infected Pigs, Permsak Thanawang, Chanida Yamkleebbua, Kritkong Shiyun, Roongroje Thanawongnuwech, Sawang Kesdangsakonwut, Supradit Wangnaitham, Sanipa Suradhat
The Thai Journal of Veterinary Medicine
Semi-quantitative analysis of the interleukin 10 (IL-10) in bronchoalveolar lavage cells (BALC), from pigs challenged with porcine reproductive and respiratory syndrome virus (PRRSV), was undertaken in this study. Twenty-one, three weeks old, crossbred pigs from a PRRSV-free commercial farm were separated into three groups, a control, non-infected group (3 pigs/group), and 2 groups (9 pigs/group), the first inoculated with a Thai-isolated European (O2SB3) strain and the second a Thai-isolated, North American (O1NP1) PRRSV strain. BALC were isolated from three infected pigs and one control on days 5, 9 and 15 post infection (DPI). The levels of IL-10 production in BALC …
Antigen Distribution Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In Thai Crossbred Pigs Using Immunohistochemistry, Porntip Laohasittikul, Nuttapon Boonarpha, Yongyut Pongprapachuen, Sawang Kesdangsakonwut, Supradit Wangnaitham, Roongroje Thanawongnuwech
Antigen Distribution Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In Thai Crossbred Pigs Using Immunohistochemistry, Porntip Laohasittikul, Nuttapon Boonarpha, Yongyut Pongprapachuen, Sawang Kesdangsakonwut, Supradit Wangnaitham, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Twenty, 3-week-old, crossbred pigs from a herd free from porcine reproductive and respiratory syndrome virus (PRRSV) were inoculated intranasally and intramusculary (1 ml each 102.5 TCID50/ml) with Thai PRRSV isolates of the US genotype (01NP1) (n=9) or the EU genotype (02SB3) (n=8). Three pigs were used as negative controls. Three infected and one control group pig were euthanatized 5, 9 or 15 days postinoculation (DPI) to detect and to compare the location and the amount of PRRSV-antigen present. Immunohistochemical evaluation of formalin-fixed tissues revealed PRRSV-antigen in the pulmonary macrophages of the lungs (6/9, 8/8 and 0/3 of the 01NP1-infected, 02SB3-infected …
Cytokine Profiles Following Mycoplasma Hyopneumoniae And Prrsv Co-Infection In Pigs, Roongroje Thanawongnuwech, Eileen L. Thacker
Cytokine Profiles Following Mycoplasma Hyopneumoniae And Prrsv Co-Infection In Pigs, Roongroje Thanawongnuwech, Eileen L. Thacker
The Thai Journal of Veterinary Medicine
Bronchoalveolar cells collected by lung lavage 28 days post infection (DPI) of negative controls, Mycoplasma hyopneumoniae (M. hyo)-infected, Porcine Respiratory and Reproductive Syndrome virus (PRRSV)-infected and dual-infected pigs, were evaluated for various cytokine mRNA (IL1α, IL1β, IL6, IL8, IL10, IL12, and TNFα). Semiquantitative RT-PCR revealed that IL1β, IL6, IL10 and the IL12 mRNA expression was significantly increased in dual-infected pigs, compared with the negative controls. PRRSV-infected pigs had significantly increased levels of IL10 and IL12 mRNA. The increased IL10 gene expression that was observed in the dual infected pigs may play a role in the potentiation of PRRSV-induced pneumonia by …
The Role Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In The Porcine Respiratory Disease Complex (Prdc), Roongroje Thanawongnuwech
The Role Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In The Porcine Respiratory Disease Complex (Prdc), Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Concurrent bacterial infections of the respiratory tract in porcine reproductive and respiratory syndrome virus (PRRSV)-infected pigs is a major problem for the swine industry and may involve various factors. This review manuscript discusses the role of pulmonary macrophages, which are affected by PRRSV. The macrophage's phagocytic and bactericidal ability is altered by the PRRSV infection as is pulmonary clearance. PRRSV also induces apoptosis and macrophages cell lysis leading to an increased susceptibility to secondary bacterial infection, and the syndrome called porcine respiratory disease complex (PRDC). Prevention and control strategies for these concurrent infections are briefly discussed.