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Full-Text Articles in Medicine and Health Sciences
Detection Of Streptococus Equi From Environmental Samples Using An Elisa, Taryn Haller
Detection Of Streptococus Equi From Environmental Samples Using An Elisa, Taryn Haller
Honors College
Equine Strangles, an upper respiratory disease caused by Streptococcus equi subspecies equi (S. equi) causes significant losses in the horse industry. Surveillance for S. equi could be facilitated by quantifying S. equi in environmental samples. The objective of this project was to evaluate ELISA in quantifying S. equi using two monoclonal antibodies (MAB’s) to the SeM protein (MAB-212 for capture, and biotinylated MAB-211 for detection), with Streptococcal phage lysin releasing SeM from the cell surface. Initial results confirmed a fresh culture of S. equi and two S. equi lysates stored at -20 C for 2 years as positive, while confirming …
Improved Detection Of Streptococcus Equi Subspecies Equi In Drinking Water, Lily A. Mclaughlin
Improved Detection Of Streptococcus Equi Subspecies Equi In Drinking Water, Lily A. Mclaughlin
Honors College
Streptococcus equi subspecies equi (S. equi) is the causative agent of strangles, a contagious respiratory disease of horses. Transmission of the bacteria can occur when animals share water sources. Detection of S. equi in water could improve strangles surveillance and move towards eradication of the disease. The aims of this study were to determine the optimal membrane pore size for bacterial retention from an aqueous suspension, to determine the likely dispersion pattern of S. equi contaminated mucus in a water bucket to develop a collection technique to be used by veterinarians, and to find the sensitive range for …