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Veterinary Medicine

Chulalongkorn University

Salmonella

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Full-Text Articles in Medicine and Health Sciences

Occurrence And Characteristics Of Salmonella Isolated From Various Vegetable Sources: Potential For The Human-Food Interface In Salmonellosis In Vientiane, The Capital Of Laos Pdr, Dethaloun Meunsene, Thanaporn Eiamsam-Ang, Prapas Patchanee, Ratchadaporn Boripun, Phacharaporn Tadee, Sayaphet Rattanavong, Manivanh Vongsouvath, Pakpoom Tadee Jun 2022

Occurrence And Characteristics Of Salmonella Isolated From Various Vegetable Sources: Potential For The Human-Food Interface In Salmonellosis In Vientiane, The Capital Of Laos Pdr, Dethaloun Meunsene, Thanaporn Eiamsam-Ang, Prapas Patchanee, Ratchadaporn Boripun, Phacharaporn Tadee, Sayaphet Rattanavong, Manivanh Vongsouvath, Pakpoom Tadee

The Thai Journal of Veterinary Medicine

Salmonella is one of the most frequently reported causes of bacterial foodborne outbreaks in the world. Despite this, cases associated with raw vegetable consumption have increased, however very few studies have focused on this. The present study is an overview of the role of vegetables as a concern for Salmonella circulating in Vientiane, the capital of the Lao People's Democratic Republic (Lao PDR). A cross-sectional study was surveyed during the first half 2019 on various types of vegetable sold in retailers in the area. The prevalence, serotype, antimicrobial resistance pattern and genetic profile of Salmonella were investigated. In this study, …


Distribution Of Salmonella Spp. And Serovars Isolated From Captive Reptiles In Four Zoos Of The Zoological Park Organization, Thailand, Between 2017 And 2018, Sompoth Weerakhun, Paweena Kongsanan, Wichit Kongkham Jun 2022

Distribution Of Salmonella Spp. And Serovars Isolated From Captive Reptiles In Four Zoos Of The Zoological Park Organization, Thailand, Between 2017 And 2018, Sompoth Weerakhun, Paweena Kongsanan, Wichit Kongkham

The Thai Journal of Veterinary Medicine

Salmonellosis is the main reason for food borne diseases in many countries globally and it has been found that reptiles are carriers of the disease. Therefore, this study was performed to determine the distribution of Salmonella spp. and their serovars from reptiles in zoos in Thailand between 2017 and 2018. A total of 257 reptile samples were collected from four zoos which are Dusit (128 samples), Chiangmai (32 samples), Songkhla (34 samples) and Nakhonratchasima (63 samples). Salmonella was isolated and identified, serotyping of those isolates was investigated using a panel of Salmonella antisera and serotypes were characterized according to the …


Therapeutic Potential Of Bulgarian Propolis Against Antibiotic-Resistant Salmonella Species, Manar M. Farouk, Amal El-Molla, Fayez A. Salib, Yousef A. Soliman, Mohamed Shaalan Mar 2022

Therapeutic Potential Of Bulgarian Propolis Against Antibiotic-Resistant Salmonella Species, Manar M. Farouk, Amal El-Molla, Fayez A. Salib, Yousef A. Soliman, Mohamed Shaalan

The Thai Journal of Veterinary Medicine

The emergence of multidrug-resistant (MDR) Salmonella spp. has increased the need for investigation of antimicrobial alternatives. Ethanolic extract of propolis (EEP) has been identified to have wide-ranging medical applications related to its antimicrobial activity. The current study aimed to identify the major chemical components of EEP with antibacterial effects, evaluate the antibacterial efficacy of EEP against MDR Salmonella spp. isolates, and further examine the therapeutic efficacy of EEP against MDR S. Enteritidis infected mouse model. Bulgarian propolis EEP was prepared by using ethanol based extraction method. The chemical composition of EEP was characterized using the gas chromatography-mass spectroscopy (GC-MS). The …


Seasonal And Antimicrobial Resistance Distributions Of Salmonella Isolated From Pork, Beef And Chicken Meat In Vientiane Capital, Lao Pdr, Sitthasone Xaymountry, Kanit Chukanhom, Seri Klangair, Jatesada Jiwakanon, Sunpetch Angkititrakul Mar 2022

Seasonal And Antimicrobial Resistance Distributions Of Salmonella Isolated From Pork, Beef And Chicken Meat In Vientiane Capital, Lao Pdr, Sitthasone Xaymountry, Kanit Chukanhom, Seri Klangair, Jatesada Jiwakanon, Sunpetch Angkititrakul

The Thai Journal of Veterinary Medicine

The objectives in this study was to investigate the prevalence, seasonal variation and antimicrobial resistance of Salmonella isolated from pork, beef and chicken meat at retail markets in Vientiane capital, Lao PDR. During May 2018 to April 2019, 445 swab samples were randomly collected from pork (n = 258), beef (n = 81) and poultry meat (n = 106) in three seasons at three retail big markets in Vientiane capital, Lao PDR, Thongkhankham, Lao-Aussi, and Nongchan markets. All samples were isolated and identified for Salmonella spp. using the ISO 6579:2002/AMD 1:2007 procedure. The isolates were tested for antimicrobial susceptibility using …


Occurrence And Sequence Type Of Antimicrobial Resistant Salmonella Spp. Circulating In Antibiotic-Free Organic Pig Farms Of Northern-Thailand, Pakpoom Tadee, Prapas Patchanee, Ben Pascoe, Samuel K. Sheppard, Dethaloun Meunsene, Tunyamai Buawiratlert, Phacharaporn Tadee Jun 2021

Occurrence And Sequence Type Of Antimicrobial Resistant Salmonella Spp. Circulating In Antibiotic-Free Organic Pig Farms Of Northern-Thailand, Pakpoom Tadee, Prapas Patchanee, Ben Pascoe, Samuel K. Sheppard, Dethaloun Meunsene, Tunyamai Buawiratlert, Phacharaporn Tadee

The Thai Journal of Veterinary Medicine

Salmonella is one of the major foodborne pathogens, often as the result of pork consumption. Currently, with the situation of antimicrobial resistance, organic farming has been suggested as an alternative for healthier options. However, there is little evidence to support this. In this study, we investigated the occurrence of Salmonella circulating in local organic pig farms in northern Thailand and typed isolated clones to better understand the population structure and transmission dynamics of the underlying Salmonella contamination. In total, 112 samples from 11 organic pig farms were processed from October to December 2018. Salmonella was detected in 9 targeted farms. …


Prevalence, Risk Factors And Antimicrobial Resistance Of Salmonella Infection In Turtles In Khon Kaen Province, Patchanee Sringam, Peerapol Sukon, Sarawut Sringam, Sunpetch Angkititrakul Jun 2021

Prevalence, Risk Factors And Antimicrobial Resistance Of Salmonella Infection In Turtles In Khon Kaen Province, Patchanee Sringam, Peerapol Sukon, Sarawut Sringam, Sunpetch Angkititrakul

The Thai Journal of Veterinary Medicine

Considering the impact of Salmonella in turtles and the role of turtles as a source of Salmonella outbreaks in humans, knowledge of Salmonella status in turtles is important. A cross-sectional study was conducted to investigate the prevalence, risk factors and antimicrobial resistance of Salmonella infections in turtles. Cloacal swabs were collected from 378 turtles in Turtle Villages located in Khon Kaen province of northeastern Thailand between January 2018 and April 2019. All samples were examined for Salmonella spp. isolation and identification by ISO 6597:2002. The overall prevalence of Salmonella in the turtles was 37.57% (142/378). Habitat, season and sex were …


Prevalence Of Salmonellaspp. And Escherichia Coliin Dogs With Diarrhoea In Western Taiwan, Chiu-Huang Ting, Chia-Ying Lin, Chia-Wen Chung, Perng-Chih Shen, Ching-Dong Chang, Hung-Yi Wu Jun 2020

Prevalence Of Salmonellaspp. And Escherichia Coliin Dogs With Diarrhoea In Western Taiwan, Chiu-Huang Ting, Chia-Ying Lin, Chia-Wen Chung, Perng-Chih Shen, Ching-Dong Chang, Hung-Yi Wu

The Thai Journal of Veterinary Medicine

Salmonellaspp. can be widely spread by air or contaminated food. There are sixcommon pathogenic Escherichia coli whichaccount for 4–8% of all E. coli pathogens. Amongstthem, E. coliO157 is the most commonlyknown. In this study, we investigated the prevalence of diarrhoeapathogens, referred to as Salmonellaspp.andEscherichia coli(E. coli), in pet dogs in western Taiwan using the conventionalpolymerase chain reaction (PCR). The related analysis between the prevalence rates and the epidemiological data of pet dogs was correlated with age, season, area and breed. Faecalsamples were collected from240dogs which had symptoms of diarrhoeaand had been examined byveterinaryhospitals from January 2015 to January 2017. The …


Effects Of Copper-Bearing Montmorillonite On Salmonella Spp. In Vitro Study, Chiu-Huang Ting, Shih-Chu Chen, Ching-Dong Chang, Wen-Ling Shih, Perng-Chih Shen, Hung-Yi Wu Mar 2020

Effects Of Copper-Bearing Montmorillonite On Salmonella Spp. In Vitro Study, Chiu-Huang Ting, Shih-Chu Chen, Ching-Dong Chang, Wen-Ling Shih, Perng-Chih Shen, Hung-Yi Wu

The Thai Journal of Veterinary Medicine

Clay is an important material available in nature. Montmorillonite is considered viable for producing enhanced performance in a variety of materials and products. Farmed animals such as pigs and poultry receive additional Zn and Cu in their diets as supplements in their compound feed and these elements are also used for medical remedies. This study aimed to observe the bactericidal activity of copper-bearing montmorillonite (Cu-MMT) against Salmonella spp. in vitro. The experiments were divided into two methods that effect Salmonella spp.. First, Cu-MMT was used to precipitate the Cu2+ ion supernatant mixed with bacteria. The second, Cu-MMT was directly mixed …


Efficacy Of Competitive Exclusion To Reduce Salmonella In Broiler Chickens, Nattaporn Jiratitipat, Prapasri Srikhong, Wisanu Wanasawaeng, Niwat Chansiripornchai Dec 2019

Efficacy Of Competitive Exclusion To Reduce Salmonella In Broiler Chickens, Nattaporn Jiratitipat, Prapasri Srikhong, Wisanu Wanasawaeng, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

Competitive exclusion (CE) relies on the activity of normal flora to limit intestinal colonization by enteric pathogens. This study aims to investigate the effects of CEon Salmonellainfectionin broilers. The protective effect of CE on Salmonella cecal colonization was evaluated in 1 day-old chicks.In the laboratory trial, CE products were administered by oral inoculation, drinking water and whole body spray. Three days after treatment,the chickens were challenged with 107cfu/mL of SalmonellaEnteritidisorally, and were evaluated for 10 days. The CE-treated chickens showed comparable protection andthe number of Salmonella in the cecal contents significantly decreased(P<0.05)compared with those of the positive control. In the farm trial,CE was administeredby whole body spray at the hatchery and the second was administered by drinking water and evaluated at 32 and 42 days, respectively. The CE significantly reduced Salmonellacontamination in the farm trial andno Salmonellawas detected in the cecal contents compared with the control group. Average body weight gains, feed conversion ratioand theperformance indexof the chickens were greater than thecontrol group. Thus, the use of CEhad a positive effect on broiler performance. Furthermore, the CE-treated chickens displayed greater intestinal histology including villous height, submuscular layerand cell mitosis.These studies demonstrate that CEwas able to protect chickens from Salmonella cecalcolonization and it is apparent that new methodologies associated with the development of a workable CE program are needed in thepoultry industry.


Prevalence And Antimicrobial Resistance Of Salmonella And Campylobacter Species Isolated From Laying Duck Flocks In Confinement And Free-Grazing Systems, Chalermkiat Saengthongpinit, Siriporn Kongsoi, Srisamai Viriyarampa, Thaweesak Songserm Sep 2015

Prevalence And Antimicrobial Resistance Of Salmonella And Campylobacter Species Isolated From Laying Duck Flocks In Confinement And Free-Grazing Systems, Chalermkiat Saengthongpinit, Siriporn Kongsoi, Srisamai Viriyarampa, Thaweesak Songserm

The Thai Journal of Veterinary Medicine

Prevalence and antimicrobial resistance of Salmonella and Campylobacter species isolated from duck laying flocks in 7 confinement systems and 7 free-grazing systems were determined in central Thailand. In the confinement system, 13.8% (88/639) of Campylobacter spp. were isolated from cloacal swabs; 72.7% was identified as C. jejuni and 27.3% was identified as C. coli. From drinking and surface water samples, 60.0% of Campylobacter spp. were isolated; all was identified as C. jejuni. In the free-grazing system, 0.3% (2/700) of Campylobacter spp. were isolated from cloacal swabs; all was identified as C. jejuni. In addition, 52.9% was isolated from drinking and …


Antimicrobial Use And Multidrug-Resistant Salmonella Spp., Escherichia Coli, And Enterococcus Faecalis In Swine From Northern Thailand, David C. Love, Prasit Tharavichitkul, Orapun Arjkumpa, Maho Imanishi, Soawapak Hinjoy, Kenrad Nelson, Keeve E. Nachman Mar 2015

Antimicrobial Use And Multidrug-Resistant Salmonella Spp., Escherichia Coli, And Enterococcus Faecalis In Swine From Northern Thailand, David C. Love, Prasit Tharavichitkul, Orapun Arjkumpa, Maho Imanishi, Soawapak Hinjoy, Kenrad Nelson, Keeve E. Nachman

The Thai Journal of Veterinary Medicine

The aim of this study was to isolate antimicrobial-resistant bacteria from intensive commercial and backyard swine operations (farms) in Northern Thailand and to characterize antimicrobial usage among swine farmers. A total of 51 swine farms in Chiang Mai, Lampoon, and Nan provinces were visited from February to October 2011 to collect swine fecal samples for bacterial isolation (Salmonella spp., Escherichia coli, Enterococcus faecalis) and antimicrobial resistance testing. A survey about production intensity, management practices, animal health, and antimicrobial usage was conducted with a sub-set of farms (n = 20) located in Nan province. The farm-level prevalence of drug-resistant E. coli, …


Isolation And Molecular Characterization Of Multidrug-Resistant Salmonella, Shigella And Proteus From Domestic Birds, Aly E. Abo-Amer, Mohammed Y. Shobrak Mar 2015

Isolation And Molecular Characterization Of Multidrug-Resistant Salmonella, Shigella And Proteus From Domestic Birds, Aly E. Abo-Amer, Mohammed Y. Shobrak

The Thai Journal of Veterinary Medicine

The increase in multidrug-resistant enteric bacteria, especially Salmonella, Shigella and Proteus in poultry has become a significant public health threat. Therefore, this work was aimed to investigate whether domestic birds have a role in the emergence and transmission of multidrug-resistant (MDR) bacteria in Taif province. Salmonella, Shigella and Proteus were isolated from different types of healthy domestic birds (n=42) collected from different places. Bacterial isolates were recovered from cloacal swabs of birds by non-selective and selective pre-enrichment technique. One hundred and sixty six bacterial isolates were screened for antibiotic susceptibility such as Cefaclor, Oxacillin, Ampicillin, Chloramphenicol, Cephalexin, Neomycin, Colistin, Ciprofloxacin, …


Quantitative Microbial Risk Assessment Of Salmonella In Surface Water As A Source Of Tap Water, Paweeranut Banmairuroy, Phattharaporn Chaichana, Chaiwat Pulsrikarn, Suphachai Nuanualsuwan Mar 2014

Quantitative Microbial Risk Assessment Of Salmonella In Surface Water As A Source Of Tap Water, Paweeranut Banmairuroy, Phattharaporn Chaichana, Chaiwat Pulsrikarn, Suphachai Nuanualsuwan

The Thai Journal of Veterinary Medicine

Salmonella spp. is fecal-orally transmitted and readily spread into the environment. Little is known about risk of utilizing surface water in Thailand. Quantitative microbial risk assessment has been used to estimate the risk of waterborne salmonellosis. For hazard characterization, beta-Poisson model was employed to describe the relationship between dose of Salmonella from water exposure and salmonellosis. Prevalence and concentration of Salmonella in the surface water including water exposure were used to model probability of exposure. The probabilistic prevalence was described by beta distribution and the range of mean Salmonella prevalences of the surface water along rivers were between 8.33% and …


Effects Of Effective Microorganisms On Growth Performances, Ammonia Reduction, Hematological Changes And Shedding Of Salmonella Enterica And Campylobacter Spp. In Broilers, Chackrit Nuengjamnong, Taradon Luangtongkum Mar 2014

Effects Of Effective Microorganisms On Growth Performances, Ammonia Reduction, Hematological Changes And Shedding Of Salmonella Enterica And Campylobacter Spp. In Broilers, Chackrit Nuengjamnong, Taradon Luangtongkum

The Thai Journal of Veterinary Medicine

The aim of this study was to determine the effects of effective microorganisms (EM) product on ammonia reduction, growth performances, Salmonella and Campylobacter reduction and hematological changes in broilers. Five hundred and ninety four day-old female Arbor Acres were divided into 3 groups with 6 replicates in each group. Group 1 was the control group, while groups 2 and 3 received EM product in forms of daily spraying at 1:50 EM solution/m2 on litter and in drinking water at 1:800 dilution, respectively. Ammonia concentrations were measured weekly, while blood samples were collected at 21 and 42 days of age for …


Prevalence And Genotype Of Salmonella Choleraesuis In Gunma Prefecture, Japan, Chieko Sakano, Yukio Morita, Koichi Goto, Yoko Yokota, Hideyuki Annaka, Masahiro Fujita, Satoshi Kobatake, Taisei Ishioka, Toshie Hoshino, Sumalee Boonmar, Chaiwat Pulsrikarn, Atsuyoshi Nishina, Kunihisa Kozawa, Shigeki Yamamoto, Hirokazu Kimura Sep 2011

Prevalence And Genotype Of Salmonella Choleraesuis In Gunma Prefecture, Japan, Chieko Sakano, Yukio Morita, Koichi Goto, Yoko Yokota, Hideyuki Annaka, Masahiro Fujita, Satoshi Kobatake, Taisei Ishioka, Toshie Hoshino, Sumalee Boonmar, Chaiwat Pulsrikarn, Atsuyoshi Nishina, Kunihisa Kozawa, Shigeki Yamamoto, Hirokazu Kimura

The Thai Journal of Veterinary Medicine

We studied the prevalence of swine salmonellosis and PFGE genotype of isolates in Gunma Prefecture,Japan. Between 2005 and 2008, swine salmonellosis was confirmed in 430 of 2,707,402 (0.02%) swine atslaughterhouses. All isolates were identified as deriving from Salmonella Choleraesuis, biotype Choleraesuis (negativefor H2S production). We used 30 bacterial strains from 15 farms that had experienced outbreaks in 2006 and 2007. Allstrains were susceptible to various antibiotics such as cephems (cefotaxime), fluoroquinolones (norfloxacin andciprofloxacin), and fosfomycin. On the other hand, all strains were resistant to tetracycline (TC), and 29 of 30 (97%)strains were resistant to streptomycin (SM). The most predominant profiles …


Some Effects Of Organic Acids On The Control Of Salmonella In Broiler Chickens, Indhira Kramomtong, Waree Niyomthum, Nuttee Am-In, Piriya Uttama, Vichuda Quanjai Dec 2004

Some Effects Of Organic Acids On The Control Of Salmonella In Broiler Chickens, Indhira Kramomtong, Waree Niyomthum, Nuttee Am-In, Piriya Uttama, Vichuda Quanjai

The Thai Journal of Veterinary Medicine

Three hundred and sixty day-old chicks were used in this study to investigate the effects of organic acids on the control of Salmonella. The chicks were divided into uninoculated and inoculated groups, 180 in each. Both groups were subdivided into 3 further groups of 60 chicks each. The uninoculated, control group comprised of, group 1 : given diluted organic acids 1:1000 via the drinking water, group 2 : given organic acids diluted at 1:2000 and group 3 : given water alone. Each bird in the three inoculated subgroups 4, 5 and 6 were orally dosed with 0.3 ml of brain …


The Antimicrobial Resistance Of Salmonellae Isolated From Animals, Indhira Kramomtong, Channarong Rodkhum, Waree Niyomthum Sep 2000

The Antimicrobial Resistance Of Salmonellae Isolated From Animals, Indhira Kramomtong, Channarong Rodkhum, Waree Niyomthum

The Thai Journal of Veterinary Medicine

A total of 39 salmonella isolates were obtained from organ samples taken from duck, chicken and pig carcasses on 34 farms. They were tested for resistance to 11 antimicrobial drugs. 69.2% of isolates were resistant to at least one drug. Most resistances was shown to tetracycline and sulfamethoxazole while most isolates were susceptible to norfloxacin, enrofloxacin, flumiquine and furazolidone. Ampicillin and cephapirin were effective on the salmonella isolates from ducks and chicken, whereas kanamycin was effective on the isolates from chicken and pigs. Trimethoprim was the drug of choice on the isolates from ducks. Forty eight percent of the isolates …